“…Environmental variables play a substantial role in abundance/biomass estimation by influencing the ecology of eDNA (Barnes et al., ). Variables examined have included temperature, pH, salinity, conductivity, anoxia, sediment type, and UV light (Barnes et al., ; Buxton, Groombridge, & Griffiths, ; Buxton, Groombridge, Zakaria, & Griffiths, ; Goldberg, Strickler, & Fremier, ; Keskin, ; Pilliod, Goldberg, Arkle, & Waits, ; Robson et al., ; Stoeckle et al., ; Strickler, Fremier, & Goldberg, ; Takahara et al., ; Weltz et al., ). However, these variables are not always measured and only a handful of studies have assessed their effects in ponds (Buxton, Groombridge, & Griffiths, ; Buxton, Groombridge, Zakaria, & Griffiths, ; Goldberg et al., ; Takahara et al., ).…”