Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing

Liu, Tianyi; Choi, Gihoon; Tang, Zifan; Kshirsagar, Aneesh; Politza, Anthony J; Guan, Weihua

doi:10.1016/j.bios.2022.114255

Cited by 34 publications

(40 citation statements)

References 47 publications

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“…The on-heating chip capability, temperature control, and reagent pre-loading provide the means to rapidly quantify viral loads in patient samples with zika virus [ 36 ]. The incorporation of these systems into HIV diagnostic tools have been shown to provide rapid results, from 60 to 90 min, while providing accurate results [ 37 , 38 , 39 ]. Novel HIV diagnostic technology, such as paper- and flexible material-based assays, provide a variety of designs with a simple fabrication process and the capability to be mass produced.…”

Section: Sample Preparation: a Major Challenge For Hiv Testingmentioning

confidence: 99%

Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings

et al. 2022

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Human immunodeficiency virus (HIV) is a global epidemic; however, many individuals are able to obtain treatment and manage their condition. Progression to acquired immunodeficiency syndrome (AIDS) occurs during late-stage HIV infection, which compromises the immune system, making it susceptible to infections. While there is no cure, antiretroviral therapy can be used provided that detection occurs, preferably during the early phase. However, the detection of HIV is expensive and resource-intensive when tested with conventional methods, such as flow cytometry, polymerase chain reaction (PCR), or enzyme-linked immunosorbent assays (ELISA). Improving disease detection in resource-constrained areas requires equipment that is affordable, portable, and can deliver rapid results. Microfluidic devices have transformed many benchtop techniques to on-chip detection for portable and rapid point-of-care (POC) testing. These devices are cost-effective, sensitive, and rapid and can be used in areas lacking resources. Moreover, their functionality can rival their benchtop counterparts, making them efficient for disease detection. In this review, we discuss the limitations of currently used conventional HIV diagnostic assays and provide an overview of potential microfluidic technologies that can improve HIV testing in POC settings.

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Section: Sample Preparation: a Major Challenge For Hiv Testingmentioning

confidence: 99%

Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings

et al. 2022

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“…An innovative approach to testing HIV is self-testing, which allows individuals to collect their own specimens, conduct tests, and interpret the results privately. Using 100 μL finger-prick whole blood, Liu et al [42], introduced a completely integrated nucleic acid testing (NAT) device. Microfluidic reagent cartridges and ultra-compact NAT-on-universal serial bus (USB) analyzers are included in the device.…”

Section: Graphene-based Biosensorsmentioning

confidence: 99%

“…At a 95% confidence level, they achieved 214 viral RNA copies/mL of whole blood after a turnaround time of 60 min. Their expectation is that the HIV NAT-on-USB device will be particularly useful to high-risk populations seeking private self-tests as early as possible after exposure due to its ease of use and high sensitivity [42].…”

Section: Graphene-based Biosensorsmentioning

confidence: 99%

Development of nanobiosensors for human immunodeficiency virus detection—a mini review

Mudenkattil

Girigoswami

Jayaprakash

et al. 2022

Exploration of Medicine

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Acquired immunodeficiency syndrome (AIDS) is a global disease caused by human immunodeficiency virus (HIV). About 50 million people have died worldwide due to HIV-1 infection alone. HIV is a primary sexually transmitted infection but can also spread via breastfeeding, blood transfer, organ transfer, etc. Early detection with the maintenance of the disease is the only way to reduce the spread and severity of the disease. There are many conventional techniques for the detection of the virus. Still, recently nano-based diagnostic method remains a little ahead of these techniques due to advancements in nanotechnology. Nanomaterial-based biosensors constitute a significant part of the discussion because of their high sensitivity and accuracy. Nanobiosensors like electronic nano biosensors, quantum dot (QD)-based biosensors, optical biosensors, electronic biosensors, electrochemiluminescence nanosensors, field-effect transistor (FET) biosensors, surface acoustic wave (SAW) biosensors, graphene-based biosensors, etc. have been widely used for detecting HIV in human blood samples. All these biosensors offer promising results in the detection of the virus. In this article, different types of nanobiosensors and their application in the field of diagnosis and maintenance of HIV was reviewed.

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“…[4, 5] These are generally immunoassays based on the detection of microbial antigens and/or antibodies against the causative pathogens, although nucleic acid-based assays have more recently been developed. [6] Similar to these pathogen biomarker assays, tests for several host inflammatory biomarkers of potential diagnostic and/or prognostic significance are also available. [7] The test kits comprise of a recognition layer containing the detection sites, combined with a porous membrane which draws the fluid to be tested past the detection sites by capillary action.…”

Section: Introductionmentioning

confidence: 99%

Perceptions and priorities for the development of multiplex rapid diagnostic tests for acute non-malarial fever in rural South and Southeast Asia: an international modified e-Delphi survey

Chew

Lohavittayavikant

Mayer³

et al. 2022

Preprint

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Background. Fever is a common presenting symptom in low- and middle-income countries (LMICs). It was previously assumed that malaria was the cause in such patients, but its incidence has declined rapidly. The urgent need to develop point-of-care tests for the most important causes of non-malarial acute febrile illness is hampered by the lack of robust epidemiological data. We sought to obtain expert consensus on analytes which should be prioritized for inclusion in fingerprick blood-based multiplex lateral flow rapid diagnostic tests (LF-RDTs) targeted towards four categories of patients with acute non-malarial fever in South and Southeast Asian LMICs, stratified by age (paediatric vs. adult) and care setting (primary vs. secondary care). Methodology/Principal Findings. We conducted a two-round modified e-Delphi survey. A total of 84 panellists were invited, consisting of seven each from 12 countries, divided into three regional panels (Mainland Southeast Asia, Maritime Southeast Asia, and South Asia). Panellists were asked to rank their top seven analytes for inclusion in LF-RDTs to be used in each patient category, justify their choices, and indicate whether such LF-RDTs should be incorporated into algorithm-based clinical decision support tools. Thirty-six panellists (43%) participated in the first round and 44 (52%) in the second. There was consensus that such LF-RDTs should be incorporated into clinical decision support tools. At a minimum, these LF-RDTs should be able to diagnose dengue and enteric fever in all patient categories. There was a clear preference to develop LF-RDTs for pathogens not readily detected by existing technologies, and for direct diagnosis through antigen detection. Pathogen biomarkers were prioritized over host inflammatory biomarkers, with CRP being the only one ranked consistently highly. Conclusions/Significance. Our results provide guidance on prioritizing analytes for inclusion in context-specific multiplex LF-RDTs and similar platforms for non-malarial acute febrile illness, for which there is an urgent unmet need.

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Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing

Cited by 34 publications

References 47 publications

Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings

Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings

Development of nanobiosensors for human immunodeficiency virus detection—a mini review

Perceptions and priorities for the development of multiplex rapid diagnostic tests for acute non-malarial fever in rural South and Southeast Asia: an international modified e-Delphi survey

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