Fingerprint Analysis and Multi-ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

Abstract

A method employing high performance liquid chromatography-diode array detection (HPLC-DAD) has been developed to evaluate the quality of Saposhnikoviae Radix (SR) comprehensively through fingerprint analysis and multi-ingredient determination. In the fingerprint analysis, 12 out of 15 common peaks were identified with high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) and related references. To discriminate the 15 batches of samples collected from different origins, similarity analysis and principal component analysis (PCA) were performed. Meanwhile, the simultaneous quantification of four chromones was achieved by firstly applying a method of using a single standard to determine multiple components (SSDMC) with conversion factor. The method has the advantage of feasibility, economy and simplicity compared with traditional external standard method (ESM). The method possessed desirable linearity (R 2 ≥ 0.9997), precision (RSD <4.0%), accuracy (97.4 -106.6%) and robustness. This study indicated that the method of fingerprint analysis integrated with multi-ingredient determination using the HPLC technique was reliable for the overall quality evaluation of SR.

Rapid analysis of Saposhnikovia divaricate decoction metabolism in rats by UHPLC–Q‐TOFMS and multivariate statistical analysis

“…Among them, compounds 27, 48, 51, 67 and 72 were exactly identified as prim‐ O ‐glucosylcimifugin, cimifugin, 4′‐ O ‐ β ‐ d ‐glucosyl‐5‐ O ‐methylvisamminol, norcimifugin and sec‐ O ‐glucosylhamaudol with reference standards. The fragment pathways of the chromones have been well elucidated in published references (Chen et al, ; Kong et al, ; Liao et al, ). The chromone compounds in SDD are mainly divided into two basic structures, dihydrofuranchromones and dihydropyranchromones, which have their own characteristic fragmentation pathways.…”

“…Recent pharmacological studies indicate that SD has various bioactivities, such as anti‐inflammatory, antipyretic, analgesic and antibiotic (Kong et al, ; Okuyama et al, ). The chemical constituents of SD include chromones, coumarins, essential oils, glycoside, mannitol and polyacetylenes, among which chromones and coumarins are considered to be the major constituents (Chen, Chen, Su, Jiang, & Liu, ; Kang et al, ; Li et al, ; Liao, Li, Liu, Liu, & Bi, ). So far, there have been few reports on the metabolism of orally administered Saposhnikovia divaricate decoction (SDD).…”

Progress and Challenges of Reference Standard and Its New Form: Digital Reference Standard

“…The above methods have been used in pharmacopoeia of many countries, and widely used in related substance test of PC, Chinese medicine feature, fingerprint map, and multi-component determination [18]- [29].…”

A Comprehensive Method for Quality Evaluation of Houttuyniae Herba by a Single Standard to Determine Multi-components, Fingerprint and HPTLC Method

“…A concept of single standard to determine multi-components (SSDMC) was investigated quite intensively in recent years. 28,29 With a view to finding an internal-standard substance, we attempted to use chlorogenic acid as the reference standard required in the SSDMC method. Consequently, chlorogenic acid served both as the external standard to determine the content of chlorogenic acid in HH, and as the internal standard to simultaneously determine the other eight components according to the response factor (RF).…”