First and second order derivative spectrophotometric determination of benzyl alcohol and diclofenac in pharmaceutical forms

Micalizzi, Y. C. De; Pappano, Nora B.; Debattista, Nora B.

doi:10.1016/s0039-9140(98)00080-0

Cited by 51 publications

(30 citation statements)

References 5 publications

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“…Since DMMB dye reacts with all types of sulfated GAG chains, this assay was not able to distinguish the types of GAGs present in the mushroom extracts. Although our spectrophotometric analysis confirmed the presence of GAG chains at peaks around 190-210 nm (Lima et al, 2011), we also found additional broad peaks around 240-260 nm that were related to other aromatic compounds (de Micalizzi et al, 1998). Some ions, such as calcium or sodium, or the sulfation pattern of GAGs or other contaminants can shift the peaks (McEwen et al, 2009).…”

Section: Discussionmentioning

confidence: 47%

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

Choocheep¹,

Nathip²

2018

CMUJNS

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Several kinds of cultivated or local edible mushrooms exist in northern Thailand. While their nutritional features have been extensively analyzed, the presence of phytochemicals and glycosaminoglycans has yet to be investigated. Thus, in this study, we examined the presence of phytochemicals and glycosaminoglycans from 10 cultivated or local mushrooms: milk white russula, hygroscopic earthstar, log white fungi, log black fungi, shitake mushroom, rosy russula, sajor-caju mushroom, Jew's ear, bolete, and straw mushroom. Phytochemical analysis revealed the presence of carbohydrates, amino acids, and flavonoids in the extracts from most of the mushrooms. We detected glycosaminoglycans in all of the extracts using dimethylmethylene blue (DMMB) dye-binding assay and UVVis spectrophotometry. Hygroscopic earthstar contained the most glycosaminoglycans and sajor-caju mushroom the least. However, we were unable to distinguish the types of glycosaminoglycans, which should be considered for future study. In summary, the phytochemicals found here are crucial non-animal dietary sources of carbohydrates, amino acids, and antioxidants. In addition, the glycosaminoglycans detected in the mushroom extracts in this study potentially offer a non-animal source of glycosaminoglycans that may have clinical applications, apart from their nutritive value.

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Section: Discussionmentioning

confidence: 47%

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

Choocheep¹,

Nathip²

2018

CMUJNS

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“…Both present two peaks in the UV region due to the aldehyde groups present in their basic structure. For the pharmaceutical grade preparation, there is a peak related to the presence of benzyl alcohol [38] as its excipient. It should be noted that most pharmaceutical-grade HEP3 HEP4 HEP5 Fig.…”

Section: Structure and Changes Of Heparins Under Uv Irradiationmentioning

confidence: 99%

UV photochemical synthesis of heparin-coated gold nanoparticles

et al. 2013

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Sodium salt heparins of both reactive and pharmaceutical grade were used as the reducing and stabilizing agents for the UV photochemical synthesis of gold nanoparticles. Reduction of chloroauric acid, HAuCl 4 , by heparin using UV irradiation as a catalyst is a simple, inexpensive method with direct biofunctionalization of the products for further use. Experiments using both heparin types were carried out separately. The varying synthesis parameters were the HAuCl 4 and heparin sodium concentrations. The synthesized AuNPs present spherical as well as anisotropic shapes, such as oval, triangular, hexagonal sheets, rods, and some other faceted forms, with dimensions ranging from 20 to 300 nm as well as agglomerated networks. No usage of additional surfactants or growth-inducing seeds was necessary in the syntheses.

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“…The various analytical methods have been proposed for the determination of EDTA (5-9), sorbic acid (10)(11)(12)(13)(14), and diclofenac sodium (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31). To the best of our knowledge, this study is the first report describing simultaneous determination of EDTA, sorbic acid, and diclofenac sodium in pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of EDTA, Sorbic Acid, and Diclofenac Sodium in Pharmaceutical Preparations Using High-Performance Liquid Chromatography

2013

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Abstract. A simple high-performance liquid chromatographic method for simultaneous determination of ethylenediaminetetraacetic acid (EDTA), sorbic acid, and diclofenac sodium was developed and validated. Separation was achieved on a C 18 column (10 cm×4.6 mm) using gradient elution. The mobile phase consisted of acetonitrile-ammonium dihydrogen phosphate buffer solution (0.01 M, pH02.5, containing 0.8% tetra-n-butyl ammonium hydroxide). The detector wavelength was set at 254 nm. Under these conditions, separation of three compounds was achieved in less than 10 min. The effect of two metal salts and metal concentration on peak area of EDTA was investigated. The pH effect on retention of EDTA and sorbic acid was studied. The method showed linearity for EDTA, sorbic acid, and diclofenac in the ranges of 2.5-100.0, 5.0-200.0, and 20.0-120.0 μg/mL, respectively. The within-and between-day relative standard deviations ranged from 0.52 to 1.94%, 0.50 to 1.34%, and 0.78 to 1.67% for EDTA, sorbic acid, and diclofenac, respectively. The recovery of EDTA, sorbic acid, and diclofenac from pharmaceutical preparation ranged from 96.0-102.0%, 99.7-101.5%, to 97.0-102.5%, respectively. To the best of our knowledge, this is the first report about simultaneous determination of EDTA, sorbic acid, and diclofenac.

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First and second order derivative spectrophotometric determination of benzyl alcohol and diclofenac in pharmaceutical forms

Cited by 51 publications

References 5 publications

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

UV photochemical synthesis of heparin-coated gold nanoparticles

Simultaneous Determination of EDTA, Sorbic Acid, and Diclofenac Sodium in Pharmaceutical Preparations Using High-Performance Liquid Chromatography

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