First characterization of CTX-M-15 and DHA-1 -lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

Abstract

Extended-spectrum β-lactamases (ESBL) are a problem of great concern owing to the potential transmission of resistance to others bacterial species. Our study aims to investigate the ESBL and plasmid-mediated β-lactamases produced by Klebsiella pneumoniae strains isolated from 2010 to 2012, among patients hospitalized in "Ahemida Ben Adjila" hospital, Laghouat, Algeria, and to seek a possible clonal dissemination. Antimicrobial susceptibility testing was performed by the agar diffusion method, the characterization of resistance genes to β-lactam antibiotics was performed by PCR amplification and gene sequencing, and molecular typing was performed by ERIC-PCR. In total, of 112 clinical strains of K. pneumoniae isolated, nine isolates produced an ESBL. Antibiotics susceptibility testing showed a complete resistance to the majority of third-generation cephalosporins and a very frequent resistance to aminoglycosides and fluoroquinolones resistance. PCR analysis and sequencing showed that all isolates produced ESBL CTX-M-15; three strains of them also produced the cephalosporinase DHA-1. Molecular typing showed that most strains were not related; only three strains that produced CTX-M-15 and DHA-1 had identical profiles suggesting a clonality link. This study revealed the dissemination of ESBL CTX-M-15 and plasmid-mediated AmpC cephalosporinase DHA-1 in K. pneumoniae. This is a first report of these enzymes at the Laghouat hospital.

Multidrug-resistant Klebsiella pneumoniae harboring extended spectrum β-lactamase encoding genes isolated from human septicemias

“…Our finding seems to match completely with surveys conducted on hospitals located in different parts of Europe [ 19 , 34 – 36 ]. Likewise, it was shown that this genotype has been disseminated in Asia [ 37 ] and Africa [ 38 ]. This study constitutes additional evidence that the CTX-M-15 remains the most important CTX-M enzyme in K .…”

Systematic Review and Meta-analysis on Extended-Spectrum β-lactamases Producing Klebsiella pneumoniae in Nepal

“…5,14 Recently, the CTX-M enzymes-producers have increased throughout the world. 15,16 These ESBL encoding genes are commonly found on mobile genetic components, such as plasmids, which frequently carry genes conferring resistance to other classes of antibiotics such as aminoglycosides, fluoroquinolones, chloramphenicol, and trimethoprim/sulfamethoxazole. 17,18 As a result, many of these bacterial strains are multidrug-resistant (MDR) and infection by these drug-resistant isolates increases the likelihood of treatment failure and may lead to death.…”

Genotypic Determination of Extended Spectrum β-Lactamases and Carbapenemase Production in Clinical Isolates of Klebsiella pneumoniae in Southwest Nigeria

“…Our study adds to the body of evidence that the CTX-M-15 remains the most important CTX-M enzyme in K. pneumoniae as a result of its large diffusion and relation to infections in humans. Similarly, this particular genotype is widely disseminated in Africa [ 51 ].…”