First Class A Carbapenemase Isolated from
            <i>Enterobacteriaceae</i>
            in Argentina

Radice, Marcela; Power, Pablo; Gutkind, Gabriel; Fernández, Karina; Vay, Carlos; Famiglietti, Ángela; Ricover, Nora; Ayala, Juan A.

doi:10.1128/aac.48.3.1068-1069.2004

Cited by 38 publications

(26 citation statements)

References 7 publications

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“…However, a P. aeruginosa strain producing metallo-beta-lactamases (MBLs) was recovered from a 72-year-old patient frequently exposed to health-care settings, and an E. cloacae isolate that was resistant to imipenem but susceptible to cefotaxime and ceftazidime was isolated from a patient with celiac disease who had not used antibiotics within the last three months. The phenotypic characteristics of this strain (probably NMC-A) were similar to those reported previously by Radicce et al [19]. The screening strategy using the APB test for class A carbapenemase in P. aeruginosa is not recommended.…”

Section: Discussionsupporting

confidence: 69%

Faecal carriage of ESBL-producing Enterobacteriaceae and carbapenem-resistant Gram-negative bacilli in community settings

Villar¹,

Baserni²,

Jugo³

2013

J Infect Dev Ctries

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Introduction: The aim of this study was to determine the prevalence of intestinal carriage of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae and carbapenem-resistant Gram-negative bacilli in the community in Buenos Aires, Argentina. Methodology: Faecal samples from 164 non-hospitalized patients were cultured on CHROMagar KPC and CHROMagar ESBL plates. Isolates resistant to third-generation cephalosporins or carbapenems were selected for further study. The minimal inhibitory concentration (MIC) of the isolates was determined using the E-test method. The phenotypic detection of ESBLs and carbapenemases was performed using the double-disc synergy test. Results: The rate of faecal carriage of Enterobacteriaceae resistant to third-generation cephalosporins was 26.8%. Escherichia coli represented a large majority (75%) of the isolates recovered. Thirty-three ESBL-producing isolates were detected from 31 faecal samples (18.9% of the collected specimens). Eight carbapenem-resistant Gram-negative bacilli were recovered from eight specimens (4.9%). Conclusions: This study revealed a high prevalence of faecal carriage of multidrug-resistant Gram-negative bacteria, including ESBLs, in Buenos Aires. Therefore, the use of surveillance cultures will be helpful for tracking and monitoring the spread of ESBL-producing Enterobacteriaceae within community settings.

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Section: Discussionsupporting

confidence: 69%

Faecal carriage of ESBL-producing Enterobacteriaceae and carbapenem-resistant Gram-negative bacilli in community settings

Villar¹,

Baserni²,

Jugo³

2013

J Infect Dev Ctries

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“…The IMI (for "imipenem-hydrolyzing ␤-lactamase") and NMC-A (for "not metalloenzyme carbapenemase") enzymes have been detected in rare clinical isolates of E. cloacae in the United States, France, and Argentina (149,175,181,183). NMC-A and IMI-1 have 97% amino acid identity and are related to SME-1, with approximately 70% amino acid identity (146,183).…”

Section: Chromosomally Encoded Enzymes: Sme Nmc and Imimentioning

confidence: 99%

Carbapenemases: the Versatile β-Lactamases

2007

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SUMMARY Carbapenemases are β-lactamases with versatile hydrolytic capacities. They have the ability to hydrolyze penicillins, cephalosporins, monobactams, and carbapenems. Bacteria producing these β-lactamases may cause serious infections in which the carbapenemase activity renders many β-lactams ineffective. Carbapenemases are members of the molecular class A, B, and D β-lactamases. Class A and D enzymes have a serine-based hydrolytic mechanism, while class B enzymes are metallo-β-lactamases that contain zinc in the active site. The class A carbapenemase group includes members of the SME, IMI, NMC, GES, and KPC families. Of these, the KPC carbapenemases are the most prevalent, found mostly on plasmids in Klebsiella pneumoniae. The class D carbapenemases consist of OXA-type β-lactamases frequently detected in Acinetobacter baumannii. The metallo-β-lactamases belong to the IMP, VIM, SPM, GIM, and SIM families and have been detected primarily in Pseudomonas aeruginosa; however, there are increasing numbers of reports worldwide of this group of β-lactamases in the Enterobacteriaceae. This review updates the characteristics, epidemiology, and detection of the carbapenemases found in pathogenic bacteria.

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“…Isolates were screened by polymerase chain reaction (PCR) for the presence of the molecular class A carbapenemases (bla KPC , bla GES , bla SME , bla NMC , and bla IMI1 ) (13)(14)(15)(16)(17), class B MBL (bla I MP , bla VIM and bla NDM ) (14,18,19), class D OXA β-lactamases (bla OXA-48 ) (15), ESBL genes (bla TEM , bla SHV , and bla CTX-M ), plasmid-mediated AmpC blactamase genes (bla ACC , bla FOX , bla MOX , bla DHA , bla CIT , and bla EBC ) and integron (Int1, Int2, Int3) (20)(21)(22). All PCRpositive products were sequenced by Shanghai Majorbio Bio-Pharm technology Co. (Shanghai, China), and the results were compared to the sequences available from GenBank (www.ncbi.nlm.nih.gov/blast/).…”

Section: Drug-resistance Genesmentioning

confidence: 99%