First description of B chromosomes in the family Auchenipteridae, Parauchenipterus galeatus (Siluriformes) of the São Francisco River basin (MG, Brazil)

Lui, Roberto Laridondo; Blanco, Daniel Rodrigues; Margarido, Vladimir Pavan; Filho, Orlando Moreira

doi:10.1016/j.micron.2009.03.004

Cited by 45 publications

(49 citation statements)

References 46 publications

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“…The available data in the literature for hybridization with 18S rDNA probe for Auchenipteridae are restricted to Parauchenipterus galetaus (Lui et al, 2009;Lui et al, 2010) and Glanidium ribeiroi (Fenocchio et al, 2008), which always confirmed the results presented by silver impregnation of only one marked pair. According to Ravedutti & Júlio Jr. (2001), simple NORs seem to be a feature of Auchenipteridae.…”

Section: Discussionsupporting

confidence: 65%

“…), whereas another population of this species and Ageneiosus atronasus (cited as A. brevifilis), both from Solimões River (Fenocchio & Bertollo, 1992), and Ageneiosus brevis and Ageneiosus ucayalensis, both from Catalão Lake (Santos & Nakayama, 2011), have the diploid number of 56 chromosomes. However, species from other genera that were cytogenetically studied, as Glanidium ribeiroi, Parauchenipterus galeatus and Auchenipterus osteomystax (cited as A. nuchalis) have 58 chromosomes (Fenocchio & Bertollo, 1992;Ravedutti & Júlio Jr., 2001;Fenocchio et al, 2008;Lui et al, 2009;Lui et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

et al. 2013

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Ageneiosus is the most widely distributed genus of the family Auchenipteridae among South American river basins. Although chromosome studies in the family are scarce, this genus has the largest number of analyzed species, with 2n = 54 to 56 chromosomes, differing from the rest of the family (2n = 58). This study aimed to analyze Ageneiosus inermis from the Araguaia River basin. The diploid number found was of 56 chromosomes. Heterochromatin was allocated in terminal region of most chromosomes, plus a pericentromeric heterochromatic block in pair 1, a pair distinguished by size in relation to other chromosomes pairs. AgNORs were detected in only one submetacentric chromosome pair, which was confirmed by FISH. 5S rDNA was present in only one metacentric chromosome pair. Hybridization with [TTAGGG] n sequence marked the telomeres of all chromosomes, in addition to an ITS in the proximal region of the short arm of pair 1. The repetitive [GATA] n sequence was dispersed, with preferential location in terminal region of the chromosomes. Ageneiosus has a genomic organization somewhat different when compared to other Auchenipteridae species. Evidences indicate that a chromosomal fusion originated the first metacentric chromosome pair in A. inermis, rearrangement which may be a basal event for the genus.Ageneiosus é o gênero da família Auchenipteridae mais amplamente distribuído em bacias da América do Sul. Apesar dos estudos cromossômicos nesta família serem escassos, este gênero tem o maior número de espécies analisadas, com número diploide variando de 54 a 56 cromossomos, o que difere do restante da família (2n = 58). Este estudo objetivou analisar Ageneiosus inermis da bacia do rio Araguaia. O número diploide encontrado foi de 56 cromossomos. A heterocromatina se mostrou localizada na região terminal da maioria dos cromossomos, além de um bloco heterocromático pericentromérico no par 1, um par facilmente distinguível no cariótipo pelo seu maior tamanho quando comparado aos outros pares do complemento. AgRONs foram detectadas em somente um par de cromossomos submetacêntricos, que foi confirmado pela FISH. 5S rDNA se mostrou presente em somente um par de cromossomos metacêntricos. A hibridização com a sequência [TTAGGG] n marcou os telômeros de todos os cromossomos, além de um ITS (sequência telomérica intersticial) na região proximal do braço curto do par 1. A sequência repetitiva [GATA] n se mostrou dispersa, com localização preferencial na região terminal dos cromossomos. Ageneiosus apresenta uma organização genômica um pouco diferente quando comparada a outras espécies de Auchenipteridae. As evidências indicam que uma fusão cromossômica originou o primeiro par de cromossomos metacêntricos de A. inermis, rearranjo que parece ser um evento basal para o gênero.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

et al. 2013

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“…Most species of Auchenipteridae, analyzed by C-banding of genera Ageneiosus, Auchenipterus, Glanidium, Parauchenipterus and Tatia (Ravedutti and Júlio Junior, 2001;Fenocchio et al, 2008;Lui et al, 2009Lui et al, , 2010Lui et al, , 2013a; present paper), presented the heterochromatin as preferentially located in terminal regions with pale blocks, which seems to be a characteristic of the family. One prominent heterochromatic block was observed in the short arm of the pair where the NORs are located in the species of this study.…”

Section: Discussionmentioning

confidence: 52%

“…The chromosomal studies related to the Iguazu river basin include 24 nominally valid species, and at least 10 others that had not yet been described (Kantek et al, 2007). Regarding Auchenipteridae, the chromosomal studies are still scarce, comprising only species of the genera Ageneiosus, Auchenipterus, Glanidium, Parauchenipterus and Tatia (Fenocchio and Bertollo, 1992;Ravedutti and Júlio Junior, 2001;Fenocchio et al, 2008;Lui et al, 2009Lui et al, , 2010Lui et al, , 2013a. Regarding the Iguazu river, there are only two valid species of this family, Tatia jaracatia (Pavanelli and Bifi, 2009) and Glanidium ribeiroi, both having 58 chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Karyotypic variation of Glanidium ribeiroi Haseman, 1911 (Siluriformes, Auchenipteridae) along the Iguazu river basin

et al. 2015

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The Iguazu river is a tributary of the left margin of the Paraná river, isolated from this basin about 22 million years ago with the appearance of the Iguazu Falls. The Iguazu river is characterized by high endemism due to two factors: its rugged topography and the old isolation caused by formation of the Iguazu Falls. This study analyzed cytogenetically a population of Glanidium ribeiroi collected in a region at the final stretch of this basin, by Giemsa staining, C-banding, impregnation by silver nitrate, and FISH with probes of 5S rDNA, 18S rDNA, telomeric sequence [TTAGGG] n , and [GATA] n repeats. The diploid number was equal to 58 chromosomes. The heterochromatin was present in the terminal region of almost all chromosomes. The Ag-NORs were simple and presented interstitially on the short arm of the submetacentric pair 14, which was confirmed by FISH with 18S rDNA probe. The 5S rDNA-FISH marked only the submetacentric pair 16 on the long arm in interstitial position. The FISH with [TTAGGG] n probe presented all telomeres labeled as expected, with an absence of Interstitial Telomeric Sequence (ITS). The repetitive [GATA] n sequence was dispersed throughout the genome, with preferential location in the terminal region of all chromosomes. The data obtained are discussed herein with other species of Auchenipteridae, and other previously analyzed populations of G. ribeiroi from the Iguazu river, verifying differences among these populations, which should be mainly related to the rugged topography of this basin.Keywords: geographical barriers, ribosomal DNA, Glanidium ribeiroi, Iguazu river. Variação cariotípica de Glanidium ribeiroi

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“…The prepared slides were stained with Giemsa for conventional studies, to determine the number and morphology of chromosomes. The constitutive heterochromatin (C-banding) was performed according to Sumner (1972), with some minor adjustments (Lui et al, 2009). The nucleolar organizing regions (NORs sites) were identified by fluorescence in situ hybridization (FISH), with rDNA 18S probe, according to Pinkel et al (1986).…”

Section: Methodsmentioning

confidence: 99%

Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

et al. 2016

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The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the "scabripinnis complex", Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed". Two sympatric karyomorphs were observed in Astyanax. aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter-and intraspecific chromosomal diversity in the genus Astyanax.Keywords: cytogenetics, biodiversity, B chromosomes, C-bands, NORs. Diversidade Cariotípica entre três espécies do gênero

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First description of B chromosomes in the family Auchenipteridae, Parauchenipterus galeatus (Siluriformes) of the São Francisco River basin (MG, Brazil)

Cited by 45 publications

References 46 publications

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae)

Karyotypic variation of Glanidium ribeiroi Haseman, 1911 (Siluriformes, Auchenipteridae) along the Iguazu river basin

Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

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