Calythea Schnabl & Dziedzicki has 15 known species distributed worldwide, including three species in the neotropics. The species of Calythea can be easily identified by having bright silvery grey-dusting on the thorax and abdomen, forming a contrasting pattern with the dark body. Herein, we describe two new species from the Neotropical region and present new records of the genus for Argentina, Brazil, Chile, Colombia, Ecuador and Peru. We also redescribe Calythea comis (Stein). In addition, we present an identification key for the Neotropical species and drawings of the terminalia and habitus images of the new species, C. comis, C. crenata (Bigot) and C. micropteryx (Thomson).