First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Abstract

Since Aujeszky`s disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.

Pseudorabies virus infection in hunting dogs in Oita, Japan: Report from a prefecture free from Aujeszky’s disease in domestic pigs

“…This strongly suggests that PRV can exist in the wild boar population even if the domestic pig population in a prefecture is free from AD. Wild boars would thus represent a reservoir for PRV and risk factors for the transmission of PRV to domestic pigs [ 6 , 21 , 30 ] and other animals including hunting dogs [ 5 , 17 , 20 , 21 , 22 , 23 , 25 , 26 ]. The PRV isolates identified in this study were clustered into “genotype II,” based on the nucleotide sequence of the gC gene (gC-genotype II) [ 20 , 33 , 34 ].…”

First Isolation and Molecular Characterization of Pseudorabies Virus Detected in Turkey

“…While the noti cation of infection in domestic pigs decreases in some regions of the world, PrV reported in dogs from different parts of the world is increasing [9,10,13,15,16,24]. The genomic characterization of PrV from secondary host as obtained in this study will help to trace the population diversity and infection origin for the areas where the disease has not yet been detected or is still a problem.…”

Comparative Pathology of Pseudorabies in Different Naturally and Experimentally Infected Species—A Review

“…One of the most prominent clinical features is intense pruritus, which develops mainly at the head and is followed by self-mutilation, severe skin abrasions and edematous reaction in the affected area [ 75 , 78 , 79 , 80 , 82 , 83 , 84 , 85 , 86 , 87 ] ( Figure 5 ). However, itching can be absent in animals suffering from AD [ 76 , 77 , 79 , 81 , 88 ]. Neurological impairment such as ataxia, tremor or head pressing is also reported [ 76 , 77 , 78 , 83 , 84 , 85 , 87 , 88 ].…”

“…However, itching can be absent in animals suffering from AD [ 76 , 77 , 79 , 81 , 88 ]. Neurological impairment such as ataxia, tremor or head pressing is also reported [ 76 , 77 , 78 , 83 , 84 , 85 , 87 , 88 ]. Spasms of the musculature including trismus as well as vocalization, aggressiveness and dyspnea can be present in dogs and cats [ 75 , 76 , 77 , 78 , 79 , 80 , 83 ].…”

“…Mild to severe nonsuppurative encephalitis mainly affects the brainstem [ 28 , 75 , 76 , 78 , 81 , 84 , 85 , 86 , 87 ] ( Figure 7 ). Inflammation of the cerebrum [ 85 , 88 ], midbrain and cerebellum [ 28 ] may also occur. The inflammatory response is characterized by neuronal necrosis of brain parenchyma and perivascular cuffs consisting of mainly lymphocytes and macrophages.…”