First karyotype description of Hypostomus iheringii (Regan, 1908): a case of heterochromatic polymorphism

Traldi, Josiane Baccarin; Vicari, Marcelo Ricardo; Blanco, Daniel Rodrigues; Martinez, Juliana; Artoni, Roberto Ferreira; Filho, Orlando Moreira

doi:10.3897/compcytogen.v6i2.2595

Cited by 26 publications

(25 citation statements)

References 38 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This condition, that we found for the 18S rDNA site of chromosome pair 15 in H. ancistroides, was previously observed in H. regani (Mendes-Neto et al, 2011) and H. iheringii (Traldi et al, 2012). Possibly, it is due to the limited sensitivity of the technique for detecting very small sites (Schwarzacher and Heslop-Harrison, 2000).…”

Section: Physical Mapping Of Ribosomal Genes In Hypostomussupporting

confidence: 51%

“…FISH confirmed the variation of the 18S rDNA sites noticed in species of Hypostomus and made possible the identification of the numerical and positional diversity of rDNA 5S sites (Kavalco et al, 2004;Mendes-Neto et al, 2011;Rubert et al, 2011;Traldi et al, 2012).…”

Section: Discussionmentioning

confidence: 83%

“…In Hypostominae, the diploid number (2n) varies from 34 chromosomes in Ancistrus cuiabae (Mariotto et al, 2011) to 84 chromosomes in Hypostomus sp 2 (Cereali et al, 2008). Most cytogenetic studies show great variability in some chromosome features; however, molecular analyses, such as physical mapping of ribosomal genes, have been done for only a small number of species (Mariotto et al, 2011;Mendes-Neto et al, 2011;Rubert et al, 2011;Ziemniczak et al, 2012;Traldi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

Traldi¹,

Blanco²,

Vicari³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We examined chromosomes of three species of the genus Hypostomus, in order to contribute to the understanding of the karyotype evolution of this group. Specimens of H. ancistroides and H. nigromaculatus displayed differences in karyotype formulas, distribution and location of heterochromatin and nucleolus organizer regions when compared to other populations of the same species. We made the first cytogenetic characterization of H. tapijara, an endemic species in the Ribeira de Iguape River. These specimens had 2n = 66 chromosomes, while H. ancistroides showed 2n = 68 and H. nigromaculatus 2n = 76 chromosomes. Physical mapping of 18S and 5S rDNA sites of the three species showed simple, multiple and syntenic clusters. Synteny of ribosomal sites was found in H. ancistroides and H. tapijara, and an interspersed pattern between these sites in all chromosomes bearing the synteny was observed. We conclude that the genus Hypostomus has a high chromosome complexity that is accompanied by great morphological variation. It is evident that this group comprises an interesting model for understanding the chromosome evolution of Neotropical ichthyofauna.

show abstract

Section: Physical Mapping Of Ribosomal Genes In Hypostomussupporting

confidence: 51%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

Traldi¹,

Blanco²,

Vicari³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Some studies have suggested that the heterochromatin in CP regions might suppress or silence gene expression by the reversible transformation between heterochromatin and euchromatin [17,18]. In addition, other reports have indicated that the heterochromatin located at centromeres plays an essential role in cell division.…”

Section: Discussionmentioning

confidence: 99%

The effect of chromosomal polymorphisms on the outcomes of fresh IVF/ICSI–ET cycles in a Chinese population

Zhang

Wang

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Chromosomal polymorphisms (CPs) have been reported to be associated with infertility; however, their effects on the outcomes of in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) are still controversial. In this retrospective study, we aimed to evaluate the effect of CPs on IVF/ICSI-ET outcomes. Methods To investigate whether CPs affected the outcomes of fresh IVF/ICSI-ET cycles in a Chinese population, we evaluated infertile couples with male carriers of CPs (n = 348), infertile couples with female carriers (n = 99), and unaffected couples (n = 400) who had received their first treatment cycles in our hospital between January 2013 and March 2015.Results CPs in either male or female carriers seemed to have adverse effects on IVF/ICSI-ET outcomes. CPs in male carriers affected outcomes mainly by decreasing the rates of fertilization, embryo cleavage, good quality embryos, clinical pregnancies, ongoing pregnancies, and deliveries as well as increasing the biochemical pregnancy rate (P < 0.05); CPs in female carriers affected outcomes only by lowering the embryo cleavage rate (P < 0.05). The mean fertilization rate of couples with male CP carriers undergoing IVF was significantly lower than that in those undergoing ICSI (61.1 versus 66.5 %, respectively; P = 0.0004). Conclusions Our data provide evidence for the involvement of CPs in the poor outcomes of fresh IVF/ICSI-ET cycles in a Chinese population. The use of ICSI might improve outcomes by increasing the fertilization rate for men with CPs.

show abstract

“…In Hypostomus, data on the physical location of 5S rDNA sites are still scarce, but variations in the number and position of these markings have been reported (Traldi et al, 2012). H. iheringii was found to have only one pair of chromosomes marked by FISH 5S (Traldi et al, 2012), H. regani had nine sites identified (Mendes-Neto et al, 2011), and H. affinis (Kavalco et al, 2005) and H. cf.…”

Section: Discussionmentioning

confidence: 99%

Karyotype structure of Hypostomus cf. plecostomus (Linnaeus, 1758) from Tapajós River basin, Southern Amazon: occurrence of sex chromosomes (ZZ/ZW) and their evolutionary implications

Oliveira¹,

Ribeiro²,

Dutra³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.Hypostomus is a group of fish with numerical and structural karyotypic variability. Among them, only six species, three of which belong to the Amazon basin, show a sex chromosome. In this study, we present the karyotype structure of Hypostomus cf. plecostomus from the Teles Pires river basin in the municipality of Alta Floresta, MT. The species has 2n = 68 and the karyotype formula 14m+ 24sm+ 14st+ 16a [fundamental number (FN) = 120] in males and 15m+ L.C. Oliveira et al. 6626©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6625-6634 (2015) 24sm+14st+15a (FN = 121) in females and sex chromosomes ZZ/ZW. Argyrophilic nucleolar organizer regions (AgNORs) were identified in two pairs of chromosomes at different positions: short arm of the pair 21and long arm of the pair 27, matching the signals displayed by 18S FISH and indicating multiple NORs. Analysis of band C detected few blocks of constitutive heterochromatin in the pericentromeric regions of most chromosomes and the telomeric regions of some pairs, including the nucleolar pair 21. However, large blocks on the long arm of the nucleolar pair 27 still stood out. GC-rich heterochromatin (CMA 3 ) was visualized only coincidently with nucleolar sites. Mapping of 5S rDNA sites with FISH revealed markings in eight chromosomes, demonstrating synteny between the 18S and 5S sites. The data obtained for H. cf. plecostomus are important for taxonomic studies of this Amazon complex "H. plecostomus group". The occurrence of sex chromosomes in Amazon species of Hypostomus suggests an evolutionary event that is independent of other species in the group.

show abstract

First karyotype description of Hypostomus iheringii (Regan, 1908): a case of heterochromatic polymorphism

Cited by 26 publications

References 38 publications

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

Chromosomal diversity in Hypostomus (Siluriformes, Loricariidae) with emphasis on physical mapping of 18S and 5S rDNA sites

The effect of chromosomal polymorphisms on the outcomes of fresh IVF/ICSI–ET cycles in a Chinese population

Karyotype structure of Hypostomus cf. plecostomus (Linnaeus, 1758) from Tapajós River basin, Southern Amazon: occurrence of sex chromosomes (ZZ/ZW) and their evolutionary implications

Contact Info

Product

Resources

About