First Molecular Survey to Detect Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms in a Strategic Production District of Sicily (South-Italy)

Galluzzo, Paola; Migliore, Sergio; Galuppo, Lucia; Condorelli, Lucia; Hussein, Hany A.; Licitra, Francesca; Coltraro, Miriana; Sallemi, Sabrina; Antoci, Francesco; Cascone, Giuseppe; Puleio, Roberto; Loria, Guido Ruggero

doi:10.3390/ani12080962

Cited by 11 publications

(12 citation statements)

References 31 publications

(37 reference statements)

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“…Real-time quantitative PCR (qPCR) is an accurate, rapid, sensitive, and cost-effective method, making it one of the most effective techniques for gene expression analysis (Harshitha et al, 2021). Galluzzo et al (2022) conducted a study to detect MG in egg-laying hens in Sicily, Italy, using real-time quantitative PCR. The study revealed an infection rate of 28.6% in commercial chicken flocks and 40.0% in rural free-range chicken flocks.…”

Section: Real-time Fluorescence Quantitative Pcrmentioning

confidence: 99%

Mycoplasma galliscepticum: An overview

Ruizhi,

Xi,

Huiqi

et al. 2024

Afr. J. Microbiol. Res.

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The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny nose and rales. Both domestic and international reports underscore MG's transmission and infection capacity, signifying its global importance as a source of poultry infection. Further research is imperative to grasp its impact on both farmed and wild animals. This article comprehensively reviews current research progress on MG, encompassing its biological characteristics, infection traits, pathogenic mechanisms, epidemiology, detection methods, treatment, and control measures. It aims to serve as a reference for comprehensive prevention and control strategies against MG in the future.

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Section: Real-time Fluorescence Quantitative Pcrmentioning

confidence: 99%

Mycoplasma galliscepticum: An overview

Ruizhi,

Xi,

Huiqi

et al. 2024

Afr. J. Microbiol. Res.

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“…Though it is a non-fatal disease 31 , it can significantly affect weight gain, feed conversion ratio, fertility, chronic respiratory disease and hatchability 32, 33 in birds. MG and MS are bacterial OIE-listed respiratory pathogens 34 which often persist in sub-clinical level 35 and are a key cause for economic loss in the poultry industry 34 . Mycoplasma infections, especially in farms with weak biosecurity, are often the cause of eggshell abnormalities and decrease in egg production 36 .…”

Section: Selected Avian Diseasesmentioning

confidence: 99%

Screening Avian Pathogens in Eggs from Commercial Hatcheries in Nepal- an Effective Poultry Disease Surveillance Tool

Sharma¹,

Dhital

Puri³

et al. 2022

Preprint

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Background: Commercial hatcheries play an important role in the overall poultry value chain- providing small to large poultry farmers with day old chicks. Any outbreak in such hatcheries can spread diseases to other farms. Regular screening of major avian pathogens, along with strict bio-security measures, can prevent spread of diseases in hatcheries. Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), Infectious Bursal Disease Virus (IBDV) and Influenza A Virus (IAV) are among the most prevalent poultry diseases which can be detected in egg albumin. Method: We retrospectively (August 2020- August 2021, except October 2020) analyzed diagnostic results for six selected avian pathogens (NDV, IBV, MS, MG, IBDV and IAV) on eggs (n=4343) received from eleven major commercial poultry hatcheries located in the five adjoining districts of Kathmandu, Nepal. Albumin from 10% randomly selected eggs from each hatchery were tested for the six avian pathogens using multiplex PCR. Result: Majority (7/11, 64%) of the poultry hatcheries had at least one of the six pathogens present. We detected at least one avian pathogen in nine out of eleven months (82%) of screening. Except for IBDV, we found one or more of the other major avian pathogens- Influenza A (IAV) (n=4 times) and Mycoplasma gallisepticum (MG) (n=4 times) were detected the most, followed by Newcastle Virus (NDV) (n=3 times). Infectious bronchitis virus (IBV) were detected twice, and Mycoplasma synoviae (MS) was detected once. Conclusion: In a resource strapped country like Nepal, poultry disease outbreak investigation in particular and surveillance in general are challenging. Meanwhile, poultry production is highly impacted by disease outbreaks often triggered by poor bio-security and lack of pathogen screening practices. Our molecular screening tests have picked up major poultry pathogens present throughout the year in eggs collected from hatcheries. Influenza A was detected at 4 different incidences throughout the year, which is of concern to both human and animal health. Quick systematic screening of eggs at key distribution points (hatcheries) for major avian pathogens is an effective surveillance tool for early disease detection and containment of outbreaks. Keywords: eggs, avian pathogens, disease screening, surveillance, poultry health

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“…2 (2023) NIWAR S. BARI AND AQEEL M. SHAREEF contaminated dust, airborne droplets and feathers, water, and the surroundings [7,8]. Direct or indirect contact through the movement of birds, people, or fomites from infected to susceptible flocks allows for easy flock-to-flock transmission [9,10]. Young birds are more vulnerable to mycoplasmosis than older birds, which can afflict birds of all ages [11].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Mycoplasma gallisepticum in Commercial Broiler Chickens in Duhok Governorate

Bari

Shareef

2023

Egyptian Journal of Veterinary Sciences

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I N the present study, indirect ELISA conducted to estimate the prevalence of MG antibodies in serum samples collected from 20 broiler flocks in Duhok governorate. Three hundred and sixty blood samples from these flocks, 126,144 and 90 serum samples were collected from broilers aged 4, 5 and 6 weeks, respectively. All samples were examined in Duhok College of veterinary medicine (Kurdistan/Iraq), from October 2021 to January 2022. The positive serum samples to MG were found in 84/126 (66.66%), 80/144 (55.55%), 32/90 (35.36%) of broilers examined at 4, 5 and 6 weeks, respectively. The overall serum positive reactors to MG were 52.48%. There was a decreasing tendency in seroprevalence with examination at 4, 5 and 6 weeks of age. Out of seven broiler flocks (A-G) examined at 4 weeks of age, three (A, B and C) show high mean ELISA MG antibodies' titer (≥5000), and only significant (P<0.05) in flock (A) (14015.3±5891.8), in comparison with all remaining five flocks (B-G). Those flocks (H-O) examined at 5 weeks of age, two flocks (H, I) show significantly (P<0.05) high mean ELISA MG antibodies' titer (≥5000) (9192.3±5944.9 and 7036±2279.3) respectively when compared with remaining six flocks (J-O). The 5 flocks (P-T) of broiler chickens examined at 6 weeks of age, only one flock (P) show significantly (P<0.05) high mean antibody ELISA MG titers (≥5000) (9357.6±6709.9). The remaining flocks (D-G), (J-O) and (Q-T) had MG antibody titers (≤5000).

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First Molecular Survey to Detect Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms in a Strategic Production District of Sicily (South-Italy)

Cited by 11 publications

References 31 publications

Mycoplasma galliscepticum: An overview

Mycoplasma galliscepticum: An overview

Screening Avian Pathogens in Eggs from Commercial Hatcheries in Nepal- an Effective Poultry Disease Surveillance Tool

Seroprevalence of Mycoplasma gallisepticum in Commercial Broiler Chickens in Duhok Governorate

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