First pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient

Abstract

In vitro maturation (IVM) of immature oocytes retrieved from surgically resected ovaries has been proposed as a method of fertility preservation in ovarian cancer patients undergoing definitive surgery. While there had been several reports of successful derivation of mature oocytes and or embryos, there have been no reports as yet of successful pregnancies. In this case report, we present a pregnancy and live birth from a young patient, with stage IIIC ovarian cancer, who had undergone fertility sparing surgery. The immature oocytes recovered after oophorectomy were fertilized after IVM. The embryos obtained were cryopreserved and later transferred to achieve a singleton healthy pregnancy leading to a live birth.

Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice

“…2). The first pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient was recently published by Prasath et al [12], followed by a second live birth reported by Segers et al at the Brussels meeting [13].…”

Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

“…A number of studies have shown that immature oocytes can be aspirated from small antral follicles visible on the surface of the ovary after excision usually with diameters exceeding 3 millimeter [4,5]. Two pregnancies resulting from cryopreserved embryos obtained from in vitro maturation (IVM) oocytes have recently been reported from women who recovered from cancer [6,7].…”

In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe

“…Since the first report of this combined technique, 13 further publications [4][5][6][7][8][9][10][11][12][13][14][15][16][17] have documented this strategy, either as a case report or in a case series (summarized in Table 1). In these publications, collection of immature oocytes was attempted in patients ranging from 0 to 44 years old and COC were identified in approximately 87 % of patients, making this an interesting procedure to augment the number of oocytes available for cryopreservation.…”

“…Published in vitro maturation (IVM)-rates of COC collected ex vivo ranged from 3 % [14] to 100 % [10,15]. From the 1583 immature oocytes collected and incubated in IVM culture media reported in literature, 351 reached the metaphase II (MII) stage (22 %).…”