“…Italy holds the highest spider diversity in Europe with 53 families, 440 genera and 1700 recorded species, 224 (13%) of which are endemic to the country (Pantini and Isaia 2019). These numbers are constantly growing thank to an effort in describing new taxa (e.g., Weiss and Sarbu 2021, Bosmans and Trotta 2021, Ballarin and Pantini 2022a,b, Isaia et al 2022, to new faunistic records (e.g., Bolognin et al 2021, Caria et al 2021, Dentici and Amata 2021, Dentici et al 2022Steinwandter et al 2022) and to the detection of allochthonous species (e.g., Di Pompeo et al 2011, Kulczycki et al 2012, Nardi et al 2019, Pantini et al 2020. DNA barcoding relies on the analysis of a specific small genomic region (for metazoans usually a fragment of the mitochondrial cytochrome c oxidase subunit 1) for molecular species identification.…”