The Nearctic family Hahniidae is seldom found in South America; only 20 species occur on the continent. Herein, we present the first record of the family in mainland Ecuador, with the description of thirteen new species in five different genera. In Amaloxenops: A. minimalista n. sp. (female); in Kasha n. gen.: Kasha patpa n. sp. (male, female); in Neohahnia: Neohahnia catleyi (female) n. sp., N. piemontana n. sp. (male, female), N. pristirana n. sp. (male, female), N. freibergi n. sp. (male, female), N. paramo n. sp. (male, female), and N. chalupas n. sp. (male); in Paramito n. gen.: Paramito papallacta n. sp. and P. oyacachi n. sp.; and in Pristirana n. gen.: Pristirana barthlotti n. sp. (male, female), P. niederi n. sp. (female), and P. nowickii n. sp. (male, female). Distribution maps are presented for all species, as well as a key to the South American Hahniidae genera.