First report and new molecular and morphological characterizations of root-knot nematode, <i>Meloidogyne javanica</i>, infecting ginger and long coriander in Vietnam

Phan, Ke Long; Le, Thi Mai Linh; Nguyen, Huu Tien; Nguyen, Thi Duyen; Trinh, Quang Phap

doi:10.21307/jofnem-2021-011

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Juvenil 2 nematodes Meloidogyne sp. A vermiform elongated body shape characterized those found in this study, having a slender straight stylet with a small round basal knob, oval and well-developed metacorpus, an overlapping esophagus with a pointed and serrated tail end intestine, and hyaline at the tail end [13]. The population of nematodes female Pratylenchus sp.…”

Section: Morphological Characters Of Phytonematodes In Ginger Cultiva...supporting

confidence: 57%

The abundance of phytonematodes associated with ginger plants in the District of Bogor, Cianjur, and Sukabumi, West Java

Almursyidi

Munif

Triwidodo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Phytonematodes are a severe threat that can cause damage to ginger plants, decreasing the quality and quantity of ginger production. Lack of information regarding ginger’s association and abundance of phytonematodes is a challenge in phytonematode management. The research objective was to study the abundance and identify associated phytonematodes in ginger plantations. Samples of rhizomes, roots, and soil were taken from symptomatic ginger plantations in major ginger production areas in Bogor, Cianjur, and Sukabumi, West Java. Phytonematodes from rhizomes and roots samples were extracted using the mist chamber technique and phytonematodes from soil samples were removed with the centrifugal flotation technique. The phytonematodes suspension obtained was observed using a stereo microscope. Identification book The Pictorial Key To Genera of Plant-parasitic Nematodes and related journals for genus and species identification were used to identify nematodes. Each nematode sample received was calculated as a phytonematodes population. The results showed that based on morphological characteristics, seven species of phytonematodes were found in the rhizomes, roots, and soil of ginger plants, namely Aphelenchoides bicaudatus, Hoplolaimus sp., Helichotylenchus sp., Meloidogyne sp., Pratylenchus sp., Radopholus similis, and Rotylenchulus reniformis. R. similis was found to dominate from Bogor’s rhizomes, roots, and soil samples. The highest nematode population was Meloidogyne sp., with at least 821 nematodes per10 g of roots associated with ginger in Sukabumi.

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Section: Morphological Characters Of Phytonematodes In Ginger Cultiva...supporting

confidence: 57%

The abundance of phytonematodes associated with ginger plants in the District of Bogor, Cianjur, and Sukabumi, West Java

Almursyidi

Munif

Triwidodo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The classification up to the generic level of PPNs was based on Decraemer and Hunt (2013). To confirm morphological identification, nuclear (18S rDNA, internal transcribed spacer [ITS] and 28S rDNA) and mitochondrial ( COI , COII /16S rDNA, and Nad5 ) gene regions of nematodes were amplified by PCR and analysed where possible (Janssen et al, 2016; Nguyen et al, 2022; Nguyen, Nguyen, et al, 2021; Phan et al, 2020). To identify tropical Meloidogyne spp., a multiplex PCR with species‐specific primers has also been employed (Kiewnick et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

“…After 1 week of fixing, nematodes were transferred to anhydrous glycerine to make permanent slides, following Seinhorst (1959). Perineal patterns of adult Meloidogyne females were mounted in glycerine following Hartman and Sasser (1985) were amplified by PCR and analysed where possible (Janssen et al, 2016;Nguyen et al, 2022;Phan et al, 2020). To identify tropical Meloidogyne spp., a multiplex PCR with species-specific primers has also been employed (Kiewnick et al, 2013).…”

Section: Materials S and Me Thodsmentioning

confidence: 99%

Diversity of plant‐parasitic nematodes (PPNs) associated with medicinal plants in Vietnam, Vietnamese PPN checklist and a pictorial key for their identification

Nguyen,

Trinh,

Nguyen

et al. 2023

Plant Pathology

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Plant‐parasitic nematodes (PPNs) are one of the most damaging pests to plants and are able to cause significant damage to all parts of plants, including stems, leaves, flowers, fruits and roots. Studies on the diversity, host range, distribution and identification methods of PPNs are therefore vital in order to create a basis for management. This current study represents the first dedicated investigation of PPNs from medicinal plants in Vietnam, focusing on the diversity of nematodes associated with 23 different plant species. In combination with a literature review of PPNs in Vietnam, this work has resulted in an updated list of 217 PPN species belonging to 40 genera, 15 families and three orders and also provides a pictorial online key for the identification of 52 most common and important PPN genera of the world. This key is based on the most crucial diagnostic features of PPN females, including female body shape, cuticle, labial shape, cephalic framework, stylet, stylet base, pharynx, median bulb, pharyngeal gland, vulva, tail shape and phasmid. Pictorial representations of these genera and their diagnostic characters are included in the browser‐based key to benefit users from all levels in nematology, be they beginners or experts.

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In silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica: a resource for studying M. javanica secreted proteins

2023

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Background Plant-parasitic nematodes (PPNs) that cause most damage include root-knot nematodes (RKNs) which are a major impediment to crop production. Root-knot nematodes, like other parasites, secrete proteins which are required for parasite proliferation and survival within the host during the infection process. Results Here, we used various computational tools to predict and identify classically and non-classically secreted proteins encoded in the Meloidogyne javanica genome. Furthermore, functional annotation analysis was performed using various integrated bioinformatic tools to determine the biological significance of the predicted secretome. In total, 7,458 proteins were identified as secreted ones. A large percentage of this secretome is comprised of small proteins of ≤ 300 aa sequence length. Functional analyses showed that M. javanica secretome comprises cell wall degrading enzymes for facilitating nematode invasion, and migration by disintegrating the complex plant cell wall components. In addition, peptidases and peptidase inhibitors are an important category of M. javanica secretome involved in compatible host-nematode interactions. Conclusion This study identifies the putative secretome encoded in the M. javanica genome. Future experimental validation analyses can greatly benefit from this global analysis of M. javanica secretome. Equally, our analyses will advance knowledge of the interaction between plants and nematodes.

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First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne javanica, infecting ginger and long coriander in Vietnam

Cited by 5 publications

References 27 publications

The abundance of phytonematodes associated with ginger plants in the District of Bogor, Cianjur, and Sukabumi, West Java

The abundance of phytonematodes associated with ginger plants in the District of Bogor, Cianjur, and Sukabumi, West Java

Diversity of plant‐parasitic nematodes (PPNs) associated with medicinal plants in Vietnam, Vietnamese PPN checklist and a pictorial key for their identification

In silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica: a resource for studying M. javanica secreted proteins

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