First Report of <i>Cotton leafroll dwarf virus</i> Infecting Upland Cotton (<i>Gossypium hirsutum</i>) in Texas

Alabi, Olufemi J.; Isakeit, Thomas; Vaughn, Robert N.; Stelly, David M.; Conner, Kassie; Gaytán, Brianna C.; Villegas, Cecilia; Hitzelberger, Christian; Santiago, Luis de; Monclova-Santana, Cecilia; Brown, Judith K.

doi:10.1094/pdis-09-19-2008-pdn

Cited by 22 publications

(13 citation statements)

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“…In addition to Africa and South America, CLRDV has also been reported in Timor-Leste [ 14 ], Thailand [ 15 ], India [ 16 ], and from Soybean aphid ( Aphis glycines ) in China [ 17 ]. Recently, there have been several reports on the detection of CLRDV in the USA: Arkansas [ 18 ], Alabama [ 19 ], Florida [ 20 ], Georgia [ 21 ], Kansas [ 22 ], Louisiana [ 23 ], Mississippi [ 24 ], North Carolina [ 25 ], Oklahoma [ 26 ], South Carolina [ 27 ] and Texas [ 28 ]. Since the first report of CLRDV, it has been detected in all the major cotton-growing counties in Georgia with 0–30% of disease incidence.…”

Section: Introductionmentioning

confidence: 99%

Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA

et al. 2021

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Cotton leafroll dwarf virus (CLRDV) is an emerging virus in cotton production in Georgia and several other Southeastern states in the USA. To better understand the genetic diversity of the virus population, the near complete genome sequences of six isolates from Georgia and one from Alabama were determined. The isolates sequenced were 5,866 nucleotides with seven open reading frames (ORFs). The isolates from Georgia were >94% identical with other isolates from the USA and South America. In the silencing suppressor protein (P0), at amino acid position 72, the isolates from Georgia and Alabama had a valine (V), similar to resistant-breaking ‘atypical’ genotypes in South America, while the Texas isolate had isoleucine (I), similar to the more aggressive ‘typical’ genotypes of CLRDV. At position 120, arginine (R) is unique to Georgia and China isolates, but absent in Alabama, Texas and South American isolates. Ten potential recombinant events were detected in the isolates sequenced. An increased understanding of CLRDV population structure and genetic diversity will help develop management strategies for CLRDV in the USA cotton belt.

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Section: Introductionmentioning

confidence: 99%

Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA

et al. 2021

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“…Cotton is one of the most economically important crops worldwide, and significant yield loses caused by cotton leafroll dwarf virus (the causal agent of cotton blue-and cotton blue-like diseases) have been reported in Brazil and Argentina [9,10,12,58,59], East Timor [13,14], India [15], Thailand [16], and, most recently, in the US [19,32]. Since 2016-2017, CLRDV has emerged and spread quickly from those sites in cotton-growing states in the southern and south-central US [19][20][21][22][23][24][25][26][27][28][29][30]32]. Despite the potential for economic importance of CLRDV, little is known about CLRDV genome-level variability and the forces that may be acting upon it.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular characterization of partial and complete genome sequences from similarly symptomatic cotton plants revealed the presence of an aphid-transmitted polerovirus named cotton leafroll dwarf virus (CLRDV) [12,14,15,17,18]. Since the first report of CLRDV infecting cotton in Alabama, US, during 2016-2017 [19], CLRDV isolates have been reported from symptomatic cotton in Georgia, Mississippi, North Carolina, Texas, Arkansas, Florida, Louisiana, Oklahoma, and South Carolina [20][21][22][23][24][25][26][27][28][29][30]. Thus, CLRDV has become recognized as an emerging polerovirus [31,32].…”

Section: Introductionmentioning

confidence: 99%

Cotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability

Ramos-Sobrinho

Adegbola

Lawrence

et al. 2021

Viruses

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Cotton leafroll dwarf virus (CLRDV) was first reported in the United States (US) in 2017 from cotton plants in Alabama (AL) and has become widespread in cotton-growing states of the southern US. To investigate the genomic variability among CLRDV isolates in the US, complete genomes of the virus were obtained from infected cotton plants displaying mild to severe symptoms from AL, Florida, and Texas. Eight CLRDV genomes were determined, ranging in size from 5865 to 5867 bp, and shared highest nucleotide identity with other CLRDV isolates in the US, at 95.9–98.7%. Open reading frame (ORF) 0, encoding the P0 silencing suppressor, was the most variable gene, sharing 88.5–99.6% and 81.2–89.3% amino acid similarity with CLRDV isolates reported in cotton growing states in the US and in Argentina and Brazil in South America, respectively. Based on Bayesian analysis, the complete CLRDV genomes from cotton in the US formed a monophyletic group comprising three relatively divergent sister clades, whereas CLRDV genotypes from South America clustered as closely related sister-groups, separate from US isolates, patterns reminiscent of phylogeographical structuring. The CLRDV isolates exhibited a complex pattern of recombination, with most breakpoints evident in ORFs 2 and 3, and ORF5. Despite extensive nucleotide diversity among all available CLRDV genomes, purifying selection (dN/dS < 1) was implicated as the primary selective force acting on viral protein evolution.

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“…The virus was first reported in the United States in Alabama in 2017 [ 8 ]. Subsequently, the virus has been reported in several cotton-producing states, i.e., Georgia, Texas, Mississippi, Louisiana, South Carolina, North Carolina, Florida, Louisiana, Arkansas, and Kansas [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Typical symptoms associated with cotton leafroll dwarf virus in South America are stunting, leaf rolling, vein yellowing, dark-green leaves, small bolls, and yield losses of up to 80% [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Prospective Alternate Hosts of an Emerging Polerovirus in Cotton Landscapes in the Southeastern United States

Pandey

Bag

Roberts

et al. 2022

Viruses

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The identification of alternate hosts that can act as virus inoculum sources and vector reservoirs in the landscape is critical to understanding virus epidemics. Cotton leafroll dwarf virus (CLRDV) is a serious pathogen in cotton production and is transmitted by the cotton/melon aphid, Aphis gossypii, in a persistent, circulative, and non-propagative manner. CLRDV was first reported in the United States in Alabama in 2017, and thereafter in several cotton-producing states. CLRDV has since established itself in the southeastern United States. The role of alternate hosts in CLRDV establishment is not clear. Fourteen common plant species in the landscape, including crops, weeds, and ornamentals (cotton, hollyhock, marshmallow, country mallow, abutilon, arrowleaf sida, okra, hibiscus, squash, chickpea, evening primrose, henbit, Palmer amaranth, and prickly sida) were tested as potential alternate hosts of CLRDV along with an experimental host (Nicotiana benthamiana) via aphid-mediated transmission assays. CLRDV was detected following inoculation in hibiscus, okra, N. benthamiana, Palmer amaranth, and prickly sida by RT-PCR, but not in the others. CLRDV accumulation determined by RT-qPCR was the highest in N. benthamiana compared with cotton and other hosts. However, aphids feeding on CLRDV-infected prickly sida, hibiscus, and okra alone were able to acquire CLRDV and back-transmit it to non-infected cotton seedlings. Additionally, some of the alternate CLRDV hosts supported aphid development on par with cotton. However, in a few instances, aphid fitness was reduced when compared with cotton. Overall, this study demonstrated that plant hosts in the agricultural landscape can serve as CLRDV inoculum sources and as aphid reservoirs and could possibly play a role in the reoccurring epidemics of CLRDV in the southeastern United States.

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First Report of Cotton leafroll dwarf virus Infecting Upland Cotton (Gossypium hirsutum) in Texas

Cited by 22 publications

References 8 publications

Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA

Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA

Cotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability

Prospective Alternate Hosts of an Emerging Polerovirus in Cotton Landscapes in the Southeastern United States

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