First Report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus on Garlic in Croatia

Vončina, Darko; Ćurić, Klara; Fabek, Sanja; Toth, Nina

doi:10.1094/pdis-08-15-0952-pdn

Cited by 13 publications

(6 citation statements)

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“…LYSV is one of the most important and widespread viruses of garlic and leek (Bos, 1981;Diekmann, 1997). As a very widespread virus, LYSV has been detected in Brazil (Fajardo et al, 2001), Argentina (Conci et al, 2002), the USA (Pappu, 2005, Testen et al, 2014, China (Chen et al, 2002), Vietnam (Ha et al, 2008), India (Gupta et al, 2013), Equador (Oleas & Arahana, 2016), Morrocco and Egypt (van Dijk, 1993), as well as in many European countries: France, Slovenia, Serbia, Croatia and Poland (Delecolle & Lot, 1981;Lot et al, 1998;Mavrič & Ravnikar, 2005;Chodorska et al, 2014;Vončina et al, 2016;Vučurović et al, 2016). In some countries, such as Israel (Salomon et al, 1996;Shiboleth et al, 2001), Greece (Dovas et al, 2001), Italy (Dovas &Vovlas, 2003), Syria (Mohammad et al, 2007) and Turkey (Fidan & Baloglu, 2009), LYSV is considered the most frequent virus.…”

Section: Introductionmentioning

confidence: 99%

Incidence and distribution of leek yellow stripe virus in allium crops in Serbia

Vučurović

Nikolić²,

Radović³

et al. 2017

PESTIC PHYTOMED

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Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic-and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.

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Section: Introductionmentioning

confidence: 99%

Incidence and distribution of leek yellow stripe virus in allium crops in Serbia

Vučurović

Nikolić²,

Radović³

et al. 2017

PESTIC PHYTOMED

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“…Onion yellow dwarf virus (OYDV) is one of the potyviruses infecting garlic in many countries including eastern EU states (Vončina et al 2016), but was never reported from Ukraine. Using double-antibody sandwich enzymelinked immunosorbent assay with virus-specific antibodies (Loewe Biochemica, Germany), OYDV was detected in 63% of samples from all four screened regions of Ukraine.…”

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confidence: 99%

First report of onion yellow dwarf virus in Ukraine

et al. 2019

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“…To date, more than 15 garlic viruses from different genera (mainly Potyvirus, Carlavirus, Allexvirus) transmitted by vegetative propagation and various vectors have been reported with significant impacts on garlic production [5]. As previously reported by Vončina et al [6] and Vončina et al [7], garlic ecotypes grown in Croatia showed a deteriorated sanitary status, especially in terms of infection rates with onion yellow dwarf virus (OYDV)-98.7%, garlic common latent virus (GCLV)-49.4% and leek yellow stripe virus (LYSV)-40.5% with only 1.3% virus-free plants. Recently, part of the garlic collection maintained under the National Programme for the Conservation and Sustainable Use of Plant Genetic Resources was tested for the presence of viruses [8].…”

Section: Introductionmentioning

confidence: 68%

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

et al. 2021

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The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (¼ of KNO3 and NH4NO3 and 2xMgSO4) containing 0.1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L−1 2,4-D + 0.5 mg L−1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L−1 2,4-D and rooted in a medium containing 0.05 mg L−1 1-naphthaleneacetic acid (NAA) + 0.005 mg L−1 6-(γ,γ-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L−1 2,4-D than those developed in media containing 1 mg L−1 2,4-D + 0.5 mg L−1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods.

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First Report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus on Garlic in Croatia

Cited by 13 publications

References 1 publication

Incidence and distribution of leek yellow stripe virus in allium crops in Serbia

Incidence and distribution of leek yellow stripe virus in allium crops in Serbia

First report of onion yellow dwarf virus in Ukraine

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

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