First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen

Abstract

Late blight, caused by Phytophthora infestans, is one of the most destructive potato diseases in the world. In Yemen, identification of P. infestans still depends on a visual survey and external examination of late blight symptoms. The objective of this study was to isolate and identify P. infestans by using advanced methods. We collected 71 disease samples and isolated the pathogen using the tuber slice method. To identify an isolated pathogen, we performed morphological characterization and gene sequence analysis of the coding genes for internal transcribed spacers. We used Koch’s hypotheses to confirm the previous results. In our study. The morphological characters of the mycelium pattern of P. infestans isolates in Yemen were profusely branching, fluffy, and white. The sporangia showed remarkable limoniform papillate sporangial shape. with average length and width of 30.6 and 28.6 µm, respectively. The sequences analysis showed high homology with a degree of identity ranging from 98 to 100% to the database sequences on GenBank. Pathogenicity tests showed that the P. infestans was the causal agent. To our knowledge, this is the first study of the isolation and characterization of P. infestans in Yemen.

Zinc oxide and copper oxide nanoparticles as a potential solution for controlling Phytophthora infestans, the late blight disease of potatoes

“…The P. infestans strain Pi Alharethi YEM 2021, identified by its GenBank accession number OQ119020, was utilized in this study. It underwent morphological, microscopic, and genetic identification, and was tested on both V8 Agar and Rye Agar [ 28 , 35 ]. V8 Agar, which exhibited favorable growth conditions, was selected for culturing P. infestans .…”

“…The aim of this study was to synthesize and characterize CuO and ZnO nanoparticles and evaluate their antifungal activities against Yemeni P. infestans isolate (Pi Alharethi YEM 2021) [ 28 ] under laboratory and greenhouse conditions.…”

“…The datasets generated and analyzed during the current study are available in the National Center of Biotechnology Information's (NCBI), The P. infestans isolates (Pi Alharethi YEM2021) has been uploaded successfully under the accession number GenBank OQ119020 website's, https://www.ncbi.nlm.nih.gov/nuccore/2417000057 [ 28 ].…”

Harnessing Soil Diversity: Innovative Strategies for Potato Blight Management in Central-Eastern Poland

“…IPM strategies are crucial for sustainable agriculture, balancing the need for crop protection with environmental and economic considerations. These practices are continually evolving with advances in plant breeding, precision agriculture, and fungicide development, offering new tools and techniques to manage late blight more effectively [2,8,18,35]. Mujica & Kroschel [36] and Kroschel et al [37] evaluated integrated pest management (IPM) versus farmer practices for controlling pests and diseases in potatoes (Solanum tuberosum L.) on the central coast of Peru.…”

“…This fusion results in the formation of a thick-walled, diploid oospore capable of long-term dormancy [8,46]. The coexistence of both mating types increased the potential for sexual reproduction and oospore formation in P. infestans, posing a serious threat to potato and tomato production worldwide [35].…”