First report on the association of <i>Pestalotiopsis mangiferae</i> with leaf blight disease of <i>Canthium dicoccum</i> in India

Mahadevakumar, S.; Janardhana, G. R.

doi:10.1111/efp.12137

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…The incidence at the field level (% tree infected) was not evaluated, but 70% to 100% have been reported in Italy [ 16 ]. Similar results have been reported for grey leaf spot disease with premature defoliation by pestalotiopsis-like fungi in other hosts, like Jatropha curcas , Canthium dicoccum , Vigna unguiculata , Cocos nucifera and Vacciniea corymbosum [ 40 , 41 , 42 , 43 , 44 ]. However, premature defoliation associated with grey leaf spot disease was not observed in the present study.…”

Section: Discussionsupporting

confidence: 86%

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

et al. 2020

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Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 Neopestalotiopsis isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four Neopestalotiopsis clades from this study and induce lesions on mango leaves. This is the first report of species of Neopestalotiopsis causing mango grey leaf spot disease in Mexico.

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Section: Discussionsupporting

confidence: 86%

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

et al. 2020

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“…It had evident that Pestalotiopsis caused minor diseases on mango and guava trees in Sri Lanka. However, many research reports revealed that Pestalotiopsis were causing widespread damage to some hardy trees like Rhododendron, conifers, Eucalyptus and Canthium dicoccumin trees in some countries (Hopkins and McQuilken , 2000;Mahadevakumar and Janardhana, 2014).…”

Section: Resultsmentioning

confidence: 99%

Fungal pathogens associated with wilting of Neolitsea cassia trees

Zuhry¹,

Rajapaksha²,

Kahawatta³

et al. 2021

Intl J Foret Ecol Environ

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Neolitsea cassia, a tree species, belongs to family Lauraceae, is endemic to Sri Lanka. Few planted trees of Neolitsea cassia were severely affected by wilt disease. Two fungal pathogens were consistently isolated from collar region and roots of symptomatic trees. These two fungal species were identified as Colletotrichum and Pestalotiopsis by microscopic observations of conidia and culture characters of isolates on PDA. This is the first report of Colletotrichum and Pestalotiopsis species causing tree wilting of Neolitsea cassia in Sri Lanka. Application of 25 l of fungicide, Thiram + Thiophanate Methyl, 50+30% WP (Homai) solution (1g/l) around the base of each infected plant as a soil drenching was effectively controlled wilting of Neolitsea cassia caused by both pathogenic fungi.

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“…Koch’s postulates were performed to confirm the organismal association with the disease, and healthy leaves were inoculated with the conidial/spore suspension of N. vitis (10 6 conidia per ml) of the isolated fungal pathogen (isolate N. vitis MZ03) on 30 leaves by detached leaf assay method by following the protocols of Mahadevakumar and Janardhana (2014) and Mahadevakumar et al . (2018).…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Neopestalotiopsis vitis associated with leaf blight disease of Manilkara zapota —a new record from India

Nuthan

Meghavarshinigowda

Maharachchikumbura

et al. 2021

Lett Appl Microbiol

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Sapota is an important horticultural crop grown in India, and Karnataka is a major producer of sapota. A characteristic leaf blight disease was observed in Southern Karnataka during field surveys conducted in 2019 with an incidence of 13–22% in approximately 45 ha of sapota field. The leaf blight‐associated pathogen was isolated on the potato dextrose agar medium. A total of 12 isolates obtained from each location were identified culturally and morphologically. Based on the morphological and cultural features, the pathogen was identified as Pestalotiopsis or Neopestalotiopsis, which was further confirmed by molecular identification using a representative isolate (MZ03). The ITS rDNA and β‐tubulin genes were amplified and sequenced using ITS1/ITS4 and T1/T22 primer pairs respectively. nBLAST search analysis and concatenated (ITS‐rDNA and TUB2 loci) phylogenetic analysis confirmed the pathogen identity as Neopestalotiopsis vitis. Pathogenicity tests conducted on detached leaves by inoculation with a conidial suspension of N. vitis produced typical blight symptoms after 4–5 days and progressed to cover the entire leaf lamina after 10–12 days. The pathogen’s identity was confirmed after re‐isolation by cultural and morphological features. Although Pestalotiopsis clavispora and Pestalotiopsis versicolor causing diseases on sapota seedlings and trees have been reported, no reports are available for the occurrence of N. vitis to sapota from India. This is the first report of N. vitis associated with leaf blight disease of sapota from India.

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First report on the association of Pestalotiopsis mangiferae with leaf blight disease of Canthium dicoccum in India

Cited by 5 publications

References 3 publications

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

Fungal pathogens associated with wilting of Neolitsea cassia trees

Morphological and molecular characterization of Neopestalotiopsis vitis associated with leaf blight disease of Manilkara zapota —a new record from India

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