First report on the isolation of Trueperella abortisuis from companion animals

Wickhorst, Jörn-Peter; Hassan, Abdulwahed Ahmed; Sammra, Osama; Alssahen, Mazen; Lämmler, Christoph; Prenger‐Berninghoff, Ellen; Naggert, M.; Timke, Markus; Rau, Jörg; Abdulmawjood, Amir

doi:10.1016/j.rvsc.2017.11.003

Cited by 13 publications

(19 citation statements)

References 10 publications

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“…Of note, in these published reports, clinical manifestations were variable, and sites of bacterial isolation did not always correlate with clinical signs. T. abortisuis has also been mentioned in the literature as an isolate from the vagina of a cow with vaginitis and metritis, 18 and, most recently, from an anal sac abscess in a dog, a perianal abscess in a cat, and urine from a cat with nephroliths and uroliths 19 . Although the pathogenic role of T. abortisuis is still to be elucidated, the available information posits that, similar to T. pyogenes , T. abortisuis is possibly a commensal opportunistic pathogen whose isolation may also suggest chronicity 13 …”

Section: Discussionmentioning

confidence: 99%

Seminal vesiculitis in a Vietnamese pot‐bellied boar

Savitske

White

Rogovskyy

et al. 2021

Vet Record Case Reports

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An 8-month-old Vietnamese pot-bellied boar was presented with a 3-week history of constipation and an enlarged scrotum. Abdominal and scrotal ultrasonography was performed to assess the gastrointestinal and genitourinary tracts. An obstructive gastrointestinal lesion was ruled out, although a large cavitated heterogeneous mass was identified dorsal to the body of the bladder, which was consistent with the location of the seminal vesicles. Culture of a sample of preputial discharge revealed growth of four bacterial types, including Escherichia coli and Trueperella abortisuis. Given the clinical findings, a diagnosis of seminal vesiculitis was made and antimicrobial therapy was initiated.

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Section: Discussionmentioning

confidence: 99%

Seminal vesiculitis in a Vietnamese pot‐bellied boar

Savitske

White

Rogovskyy

et al. 2021

Vet Record Case Reports

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“…The five T. bernardiae isolates were also investigated by sequencing the following molecular targets: 16S rRNA gene, superoxide dismutase A encoding gene sodA, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. The sequences of the oligonucleotide primers and the PCR conditions were used as previously described for 16S rRNA gene (Hassan et al 2009), gene sodA (Hijazin et al 2011(Hijazin et al , 2012c, and gene gap (Wickhorst et al 2019). The PCR products were purified and sequenced by Eurofins Genomics Germany GmbH (Ebersberg, Germany).…”

Section: Sequencing the Molecular Targetsmentioning

confidence: 99%

Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay

et al. 2021

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Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.

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“…strain 2701, was originally isolated from an anal swab of a dead male harbor seal from the Danish island of Rømø as part of a national monitoring program in 2004. Affiliation of the strain with the genus Arcanobacterium was confirmed by Gram staining, CAMP-like hemolysis, and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS; Bruker Biotyper), as described previously ( 3 , 13 – 15 ). The strain was subjected to whole-genome sequencing for phylogenetic classification.…”

Section: Announcementmentioning

confidence: 99%