First report on the molecular characteristics of mcr-1 colistin resistant E. coli isolated from retail broiler meat in Bangladesh

Ali, Md. Wohab; Utsho, Kishor Sosmith; Karmakar, Swastik; Hoque, M. Nazmul; Rahman, Md. Tanvir; Hassan, Jayedul

doi:10.1016/j.ijfoodmicro.2022.110065

Cited by 7 publications

(13 citation statements)

References 34 publications

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“…Although the location of all ARGs couldn't be confirmed due to short length sequencing, this study identified a 288 kb megaplasmid of highly transferable IncHI2 family that carries ARGs conferring resistance to aminoglycosides {aadA2, ant (3")-IIa, aph(3')-IIa, aph(3")-Ib, aph(6)-Id}, phenicols (cmlA1), sulfonamides (sul1, sul3), tetracyclines (tetA, tetR) and trimethoprims (dfrA15). Occurrence of MDR with mcr-1 E. coli has been reported earlier [9,47], however, rats carrying these types of resistance determinants on plasmids are firstly reported in this study to the best of our knowledge. Occurrence of MDR E. coli carrying ARGs on horizontally transferable elements is worrisome and imposes a potential threat of transferring and disseminating these resistance determinants to human and animals through contaminated food, water and environment.…”

Section: Plos Onementioning

confidence: 58%

“…E . coli strain 1ChBEc2mcr ( mcr-1 positive) [ 9 ] and E . coli strain ATCC25922 was used as the positive and negative controls, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Fecal pellets were suspended in 1 mL Phosphate Buffer Saline (PBS) by repeated vortexing. Serial 10-fold dilution was prepared from the fecal suspension in PBS and 100 μL of the suspension (non-diluted, 10-fold, 100-fold diluted) was spread onto MacConkey agar medium containing colistin sulfate 1 μg/mL (Fujifilm Wako Chemical Corporation, Osaka, Japan) [9]. After overnight (~16 h) incubation at 37˚C, E. coli specific lactose-fermenting colonies were streaked onto fresh MacConkey agar plates containing colistin sulfate (1 μg/mL) to obtain a pure culture.…”

Section: Isolation Of Crecmentioning

confidence: 99%

“…The multiplex PCR was conducted in a final 20 μL reaction containing 10 μL one Taq 1 Quick-Load 1 2X master mix (New England Biolabs Inc., Ipswich, MA, USA) and 5 pmol of each primer (S1 Table ). E. coli strain 1ChBEc2mcr (mcr-1 positive) [9] and E. coli strain ATCC25922 was used as the positive and negative controls, respectively. On the other hand uniplex PCR was performed for the detection of mcr-6 to mcr-10 following the in-house protocol developed by Awasthi et al…”

Section: Detection and Subtyping Of Mobile Colistin Resistance (Mcr) ...mentioning

confidence: 99%

“…Colistin resistant E. coli is widely distributed in the environment. E. coli carrying mcr genes was reported from different sources including human, street food, surface water, urban sludge, poultry meat and feces, and poultry farm environment [8][9][10][11][12][13][14]. However, no study has yet described the occurrence of colistin resistant especially mcr-1 E. coli from rodents such as rat in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

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First detection and characterization of mcr-1 colistin resistant E. coli from wild rat in Bangladesh

Ali,

Karmakar,

Utsho

et al. 2024

PLoS ONE

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Colistin resistance is a global concern warning for a one health approach to combat the challenge. Colistin resistant E. coli and their resistance determinants are widely distributed in the environment, and rats could be a potential source of these isolates and resistant determinants to a diverse environmental setting. This study was aimed to determine the presence of colistin resistant E. coli (CREC) in wild rats, their antimicrobial resistance (AMR) phenotypes, and genotypic analysis of mcr-1 CREC through whole genome sequencing (WGS). A total of 39 rats were examined and CREC was isolated from their fecal pellets onto MacConkey agar containing colistin sulfate (1 μg/ mL). AMR of the CREC was determined by disc diffusion and broth microdilution was employed to determine MIC to colistin sulfate. CREC were screened for mcr genes (mcr-1 to mcr-8) and phylogenetic grouping by PCR. Finally, WGS of one mcr-1 CREC was performed to explore its genetic characteristics especially resistomes and virulence determinants. 43.59% of the rats carried CREC with one (2.56%) of them carrying CREC with mcr-1 gene among the mcr genes examined. Examination of seventeen (17) isolates from the CREC positive rats (n = 17) revealed that majority of them belonging to the pathogenic phylogroup D (52.94%) and B2 (11.76%). 58.82% of the CREC were MDR on disc diffusion test. Shockingly, the mcr-1 CREC showed phenotypic resistance to 16 antimicrobials of 8 different classes and carried the ARGs in its genome. The mcr-1 gene was located on a 60 kb IncI2 plasmid. On the other hand, ARGs related to aminoglycosides, phenicols, sulfonamides, tetracyclines and trimethoprims were located on a 288 kb mega-plasmid separately. The mcr-1 CREC carried 58 virulence genes including genes related to adhesion, colonization, biofilm formation, hemolysis and immune-evasion. The isolate belonged to ST224 and closely related to E. coli from different sources including UPEC clinical isolates from human based on cgMLST analysis. The current research indicates that rats might be a possible source of CREC, and the presence of mcr-1 and other ARGs on plasmid increases the risk of ARGs spreading and endangering human health and other environmental components through this infamous pest.

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Section: Plos Onementioning

confidence: 58%

“…E . coli strain 1ChBEc2mcr ( mcr-1 positive) [ 9 ] and E . coli strain ATCC25922 was used as the positive and negative controls, respectively.…”

Section: Methodsmentioning

confidence: 99%

Section: Isolation Of Crecmentioning

confidence: 99%

Section: Detection and Subtyping Of Mobile Colistin Resistance (Mcr) ...mentioning

confidence: 99%