First study of sperm mediated gene transfer in Egyptian river buffalo

Hassanane, M. S.; Makawy, Aida I. El; Helalia, Sahar M.; Abdoon, Ahmed S.; Khalil, Kamal M.; Ghanem, Tharwat A.; Tohamy, Amany M.; Sun, Xiao‐Feng; Shen, Wei

doi:10.1016/j.jgeb.2017.06.003

Cited by 3 publications

(6 citation statements)

References 54 publications

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“…The SMGT method has shown successful effectiveness in generating Tg farm animals, such as pigs (Chang et al., 2002), rabbits (Shen et al., 2006), cattle (Shemesh et al., 2000), goats (Ye et al., 2008), and sheep (Pereyra‐Bonnet et al., 2011). Several standard parameters contribute to the effectiveness of SMGT, including: (1) quality of the semen sample (volume, concentration, presence of sperm with abnormal morphology, and motility); (2) DNA uptake; (3) incubation media; and (4) the assisted reproductive technique used (Hassanane et al., 2017; Lavitrano et al., 2003). The SMGT method has advantages, including low cost, ease of use, high efficiency, DNA translation, DNA functionality, and DNA transfer to the next generation (Lavitrano et al., 2002).…”

Section: Resultsmentioning

confidence: 99%

“…Hassanane et al. (2017), used DMSO to transfer the construct (pEGFP‐N1) onto the sperm of buffalo (SMGT), and the expression of GFP was observed in 74.2% of Blastocysts (Hassanane et al., 2017). In SMGT method, a study by Kurd et al.…”

Section: Resultsmentioning

confidence: 99%

“…(1) quality of the semen sample (volume, concentration, presence of sperm with abnormal morphology, and motility); (2) DNA uptake; (3) incubation media; and (4) the assisted reproductive technique used (Hassanane et al, 2017;Lavitrano et al, 2003). The SMGT method has advantages, including low cost, ease of use, high efficiency, DNA translation, DNA functionality, and DNA transfer to the next generation (Lavitrano et al, 2002).…”

Section: Evaluation Of Smgt and Tmgt Approaches To The Generation Of ...mentioning

confidence: 99%

“…In TMGT, the presence of transgenes was detected with an efficiency of 55.5%, 27%, and 80% in offspring using DMSO, DMA, and Lipofectine, respectively (Amaral et al, 2011). Hassanane et al (2017), used DMSO to transfer the construct (pEGFP-N1) onto the sperm of buffalo (SMGT), and the expression of GFP was observed in 74.2% of Blastocysts (Hassanane et al, 2017). In SMGT method, a study by Kurd et al (2018) showed that gene transfer using DMSO resulted in 62% of blastocysts having the transgene.…”

Section: Dmsomentioning

confidence: 99%

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Comparison of two methods of sperm‐ and testis‐mediated gene transfer in production of transgenic animals: A systematic review

Dehghan,

Darya,

Mehdinejadiani

et al. 2024

Animal Genetics

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Transgenic (Tg) animal technology is one of the growing areas in biology. Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell‐mediated production of Tg animals, sperm‐mediated gene transfer (SMGT), and testis‐mediated gene transfer (TMGT). However, there are currently no comprehensive studies comparing SMGT and TMGT methods, selecting appropriate gene delivery carriers (such as nanoparticles and liposomes), and determining the optimal route for gene delivery (SMGT and TMGT) for producing Tg animal. Here we aim to provide a comprehensive assessment comparing SMGT and TMGT methods, and to introduce the best carriers and gene transfer methods to sperm and testis to generate Tg animals in different species. From 2010 to 2022, 47 studies on SMGT and 25 studies on TMGT have been conducted. Mice and rats were the most commonly used species in SMGT and TMGT. Regarding the SMGT approach, nanoparticles, streptolysin‐O, and virus packaging were found to be the best gene transfer methods for generating Tg mice. In the TMGT method, the best gene transfer methods for generating Tg mice and rats were virus packaging, dimethyl sulfoxide, electroporation, and liposome. Our study has shown that the efficiency of producing Tg animals varies depending on the species, gene carrier, and method of gene transfer.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Evaluation Of Smgt and Tmgt Approaches To The Generation Of ...mentioning

confidence: 99%

Section: Dmsomentioning

confidence: 99%

See 2 more Smart Citations

Comparison of two methods of sperm‐ and testis‐mediated gene transfer in production of transgenic animals: A systematic review

Dehghan,

Darya,

Mehdinejadiani

et al. 2024

Animal Genetics

View full text Add to dashboard Cite

show abstract

“…It has been shown that sperm isolated from the epididymis when incubated with a plasmid can transfer foreign DNA to oocytes (Brackett et al , 1971). Subsequently, SMGT in chickens (Nakanishi and Iritani, 1993), buffalo (Hassanane et al , 2017), pigs (Lavitrano et al , 2002) and zebrafish (Khoo, 2000) have been reported.…”

Section: Introductionmentioning

confidence: 99%

Dimethyl sulphoxide and electrolyte-free medium improve exogenous DNA uptake in mouse sperm and subsequently gene expression in the embryo

Kurd

Hosseini

Fathi

et al. 2018

Zygote

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SummaryOne of the methods to generate transgenic animals is called sperm-mediated gene transfer (SMGT). Mature sperm cells can take up exogenous DNA molecules intrinsically and transfer them into the oocyte during fertilization. This study assessed the effect of dimethyl sulfoxide (DMSO) and electrolyte-free medium (EFM) on DNA uptake (EGFP–N1plasmid) in mouse sperm. Sperms cells cultured in human tubular fluid (HTF) without any treatment were considered as the control group. Sperms cells that were incubated in EFM and HTF with DNA/DMSO at 4°C were classified into EFM and HTF groups. Sperm motility and viability were assessed following treatment. In vitro fertilization (IVF) with sperm in all groups was performed. Fertilization, embryo development and GFP-positive blastocyst rates were analyzed and compared. The result showed that sperm motility and viability in EFM were better than those in the HTF group. The rate of development to reach the blastocyst stage and GFP-positive blastocysts was significantly higher in the EFM group compared with the HTF group (P<0.05). Our data demonstrate that sperm stored in the EFM group can improve the efficiency of SMGT for the generation of GFP-positive blastocysts.

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Simulating the effects of H2O2 and melatonin treatments on the oxidation and reduction state of sperm on two outcomes: DNA uptake in sperm and apoptosis of embryonic cells

Saberi,

Jajarmi,

Hosseini

et al. 2023

Preprint

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Improving the transfer of foreign genes into recalcitrant cells, as in the production of transgenic animal models, is a challenge. Sperm-mediated gene transfer (SMGT) is a cost-effective and rapid method for exogenous DNA transferring into egg by sperm during fertilization. This method can be improved using chemical and natural additives. The objective of this study was to examine how sperm function, DNA uptake, embryonic development, and apoptosis rates are affected by different levels of ROS under varying oxidant and antioxidant conditions in SMGT method. Sperm isolated from mice with and without plasmid were treated with varying concentrations of H2O2 and melatonin. Under oxidation conditions, sperm performance was significantly reduced compared to the melatonin treatment. Embryos from the oxidation group with 100 µM H2O2 treatment showed higher uptake of exogenous DNA, and the proportion of GFP-positive blastocysts was significantly higher than those from higher concentrations. The data demonstrate that oxidative conditions in sperm are associated with increased absorption of exogenous DNA, potentially due to an increase in the mitochondria ability resulting from reactive oxygen species increase and sperm membrane instability. The importance of antioxidants lies in their ability to safeguard DNA against oxidative stress, enhance absorption efficacy, and bolster the stability of the sperm membrane. The study showed that antioxidants reduced the risk of negative effects on sperm quality and function.

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First study of sperm mediated gene transfer in Egyptian river buffalo

Cited by 3 publications

References 54 publications

Comparison of two methods of sperm‐ and testis‐mediated gene transfer in production of transgenic animals: A systematic review

Comparison of two methods of sperm‐ and testis‐mediated gene transfer in production of transgenic animals: A systematic review

Dimethyl sulphoxide and electrolyte-free medium improve exogenous DNA uptake in mouse sperm and subsequently gene expression in the embryo

Simulating the effects of H2O2 and melatonin treatments on the oxidation and reduction state of sperm on two outcomes: DNA uptake in sperm and apoptosis of embryonic cells

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