First Trial in the Developmental Phase of the "Performance Evaluation Program" Based on the ICLAS Animal Quality Network Program: Self-Assessment of Microbiological Monitoring Methods Using Test Samples Supplied by ICLAS

Goto, Kazuo; Hayashimoto, Nobuhito; Ishida, Tomoko; Takakura, Akira; Kagiyama, Naoko

doi:10.1538/expanim.58.47

Cited by 14 publications

(15 citation statements)

References 17 publications

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“…IFA antigen slides were prepared by our center, except for the IFA tests for LCMV and hantavirus, for which the antigen slides were provided by Nagasaki university and Hokkaido university, respectively. The serological test procedures were performed by a previously described method [4]. All the strain names of bacteria and viruses used as serological antigens are listed in Table 3.…”

Section: Test Methodsmentioning

confidence: 99%

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Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan

et al. 2013

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Abstract:On the basis of our 2011 microbiological monitoring tests, we report here the current microbiological status of mice and rats housed in experimental facilities in Japan. We tested more than 14,000 mice, 6,000 serum samples, 500 fecal or cecal samples, and 200 lung samples from 3,549 mouse facilities within Japanese universities and institutes (U/I), pharmaceutical companies and contract research organizations (P/C). We also tested more than 1,500 rats, 1,600 serum samples, and 20 fecal or cecal samples from 772 U/I and P/C rat facilities. Bacterial cultures, serology, microscopy, PCR, and DNA analysis using DNA chips were performed. Staphylococcus aureus (18.8% in mouse facilities, 58.6% in rat facilities) was the most prevalent agent in both the mouse and rat facilities. The next most prevalent agents in the mouse facilities were murine norovirus (11.97%), intestinal protozoa (0.05-8.49%, from various species), Pasteurella pneumotropica (5.32%), and Helicobacter hepaticus (3.17%), while intestinal protozoa (0.74-6.84% from various species), Syphacia muris (6.20%), Pseudomonas aeruginosa (3.61%), and Pasteurella pneumotropica (3.05%) were the subsequent most prevalent agents in the rat facilities. These results suggest that the currently prevalent microbes in laboratory mice and rats in Japan are mainly opportunistic pathogens, intestinal protozoa, and microbes with low pathogenicity.

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Section: Test Methodsmentioning

confidence: 99%

“…Molecular tests: PCR tests for H. hepaticus and H. bilis as well as RT-PCR tests for MNV were performed using previously reported primers and procedures [4,5]. Fecal or cecal samples were obtained from animals and stored at −80°C until the tests were performed.…”

Section: Test Methodsmentioning

confidence: 99%

Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan

et al. 2013

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“…5). The commercial ELISA we used in this study is a standard test for diagnosis of M. pulmonis infection in laboratory mice and rats by International Council for Laboratory Animal Science Monitoring Center in Japan [11, 13]. Our results indicate that the P46L-GST ELISA we have developed is appropriate for determining the serological state of sera from M. pulmonis -infected mice and rats.…”

Section: Discussionmentioning

confidence: 88%

“…Using purified P46L-GST, we developed an ELISA and compared it with a commercial ELISA. In the commercial ELISA we tested, the antigens used were untreated but cultured bacterial cells [11]. Our findings show that the P46L-GST ELISA was able to detect antibodies against P46L-GST (Fig.…”

Section: Discussionmentioning

confidence: 96%

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Development of an ELISA Using a Recombinant P46-Like Lipoprotein for Diagnosis of <i>Mycoplasma pulmonis</i> Infection in Rodents

Asano

Torigoe

Sasaki

et al. 2014

The Journal of Veterinary Medical Science

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Mycoplasma pulmonis is one of the most prevalent bacterial pathogens that infects laboratory mice and rats. To develop an M. pulmonis-specific antigen for serological diagnosis, we cloned the cDNA of P46-like lipoprotein (P46L), an M. pulmonis putative periplasmic protein. P46L is a homolog of P46, an M. hyopneumoniae antigen. We produced recombinant P46L fused to glutathione S-transferase (GST) in Escherichia coli. Immunoblot analysis revealed that sera from Mycoplasma-infected mice and rats contained anti-P46L antibodies. We developed an ELISA using the recombinant P46L-GST protein as an antigen. Thirteen of the 14 samples from rats naturally infected with M. pulmonis were determined to be positive according to the commercial ELISA (MONILISA Myco) and positive by our ELISA. Furthermore, 18/19 samples from mice experimentally infected with M. pulmonis were positive using our P46L-GST ELISA. In contrast, only 8/19 samples from infected mice were positive by the commercial ELISA. Our results indicate that P46L-GST was an appropriate antigen for developing a serological test to determine M. pulmonis infection in laboratory mice and rats.

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ICLAS LAQ Network for the Promotion of Animal Quality in Research

et al. 2021

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ICLAS Laboratory Animal Quality Network (LAQN) programs currently consist of the Performance Evaluation Program (PEP), which focuses on microbial monitoring by and for laboratory animal diagnostic laboratories, and the Genetic Reference Monitoring Program (GENRef), which provides assay-ready reference DNA for genetic testing of mouse strains. Since 2008, PEP has grown to become a truly international program with participating laboratories in 5 continents. Launched in 2016, GENRef currently distributes DNA from 12 common inbred mouse strains for use in genetic monitoring of locally inbred colonies as well as for genetic testing of stocks, particularly genetically engineered stocks, of uncertain origins. GENRef has the capacity to include additional strains as well as additional species. PEP and GENRef provide the reagents at cost, as a resource to the international scientific community, in the interest of improving research quality in an environment of growing concern for research quality, rigor, and reproducibility.

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First Trial in the Developmental Phase of the "Performance Evaluation Program" Based on the ICLAS Animal Quality Network Program: Self-Assessment of Microbiological Monitoring Methods Using Test Samples Supplied by ICLAS

Cited by 14 publications

References 17 publications

Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan

Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan

Development of an ELISA Using a Recombinant P46-Like Lipoprotein for Diagnosis of <i>Mycoplasma pulmonis</i> Infection in Rodents

ICLAS LAQ Network for the Promotion of Animal Quality in Research

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