First-trimester fetal chromosomal diagnosis using endocervical lavage: A negative evaluation

Goldberg, Marshall F.; Chen, Andrew T. L.; Ahn, Young W.; Reidy, John A.

doi:10.1016/0002-9378(80)90142-8

Cited by 38 publications

(11 citation statements)

References 7 publications

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“…As mentioned in the "Introduction", Rhine at al. (1977) were able to detect fetal cells in a proportion of cultured TCC samples, but these results were not confirmed by Goldberg et al (1980), despite their having seen trophoblastic cellular elements in the original samples. The reason for this discrepancy is probably the length of culture time; the longer the cells are kept in culture, the more likely would be the presence of maternal cells.…”

Section: In-vitro Culture Of Tcc Samplesmentioning

confidence: 92%

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Fetal cells in transcervical samples at an early stage of gestation

Adinolfí

Sherlock

2001

J Hum Genet

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Several investigations are in progress with the aim of performing prenatal diagnosis of inherited disorders by noninvasive or minimally invasive techniques. The most important approaches are based on the detection of fetal nucleated cells in maternal blood, the analysis of fetal DNA present in maternal plasma, and the identification and isolation of fetal trophoblastic cellular elements shed into the uterine cavity and the endocervical canal. In this review, we discuss the methods that have been employed for the collection of the transcervical samples at an early stage of gestation and the techniques used for the identification of fetal cells. We also report the results of using endocervical cells for the detection of fetal chromosomal disorders by fluorescent in-situ hybridization and for performing prenatal diagnosis of fetal Rh(D) phenotypes. Recent investigations have also shown that -after the isolation of trophoblastic cells from maternal contaminants by micromanipulation -transcervical samples can be employed for the prenatal diagnosis of single gene defects, such as those causing thalassemia and sickle cell anemia. Although the present results are promising, further investigations are required to demonstrate the feasibility of performing accurate diagnosis of fetal diseases by this minimally invasive approach in all transcervical samples retrieved at an early stage of gestation.

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Section: In-vitro Culture Of Tcc Samplesmentioning

confidence: 92%

“…However, using the same procedure, Goldberg et al (1980) detected only cells in metaphase with maternal chromosome banding patterns, although they had observed trophoblastic cellular elements in the original samples.…”

Section: Collection Of Tcc Samplesmentioning

confidence: 99%

Fetal cells in transcervical samples at an early stage of gestation

Adinolfí

Sherlock

2001

J Hum Genet

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“…Several more studies were published up to 1980 with conflicting results (Bobrow and Lewis, 1971;Warren et al, 1971;Tsuji and Sasaki, 1973;Goldstein et al, 1973;Manuel et al, 1974;Rhine et al, 1975Rhine et al, , 1977Rhine and Milunsky, 1979;Amankwah and Bond, 1977;Varner et al, 1977;Goldberg et al, 1980), reviewed by Adinolfi (1995). More recently, the emergence of new technology, e.g.…”

Section: Discussionmentioning

confidence: 99%

Detection of Cells of Fetal Origin From Transcervical Irrigations

1997

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“…It should also be simple to perform and cost effective, with minimal inter-observer variability. A number of techniques have been devised to retrieve TCC samples from the endocervical canal and the endometrial cavity, including smears obtained with cotton swabs or a cytobrush (14–16), aspiration of cervical mucus with a catheter (17–19), endometrial biopsy with a Pipelle (20), and lavage of the endocervical canal (21–24) or the uterine cavity (14;22;25), all with variable success (Table 1). …”

Section: Retrieval Of Trophoblast Cells From the Cervixmentioning

confidence: 99%

Transcervical retrieval of fetal cells in the practice of modern medicine: a review of the current literature and future direction

Imudia

Kumar

Diamond

et al. 2010

Fertility and Sterility

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Objective-To review published methods for transcervical collection of fetal cells and to assess the potential of this approach for application in prenatal diagnosis.Design-Retrospective analysis of efforts at prenatal diagnosis using trophoblast cells shed into the lower uterine pole that accumulate within the cervical mucus at the level of the internal os.Results-Minimally invasive techniques that include cervical mucus aspiration, cervical swabbing, and cervical or intrauterine lavage can be used to retrieve trophoblast cells during the first trimester for diagnostic purposes, including prenatal genetic analysis. Fetal cells have been identified in these specimens with success rates that vary from 40 to 90%. The disparity in reported success rates can be a function of gestational age, collection method, operator variability, detection sensitivity, or pregnancy status. Molecular approaches have been devised to determine fetal sex and identify aneuploidies. Antibody markers have proven useful to select trophoblast cells for genetic analysis and to demonstrate that the abundance of recoverable fetal cells diminishes in abnormal gestations, such as ectopic pregnancy and blighted ovum.Conclusions-Transcervical collection of fetal cells offers several avenues for prenatal diagnosis that with further refinement could one day provide valuable information for the management of ongoing pregnancies.

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First-trimester fetal chromosomal diagnosis using endocervical lavage: A negative evaluation

Cited by 38 publications

References 7 publications

Fetal cells in transcervical samples at an early stage of gestation

Fetal cells in transcervical samples at an early stage of gestation

Detection of Cells of Fetal Origin From Transcervical Irrigations

Transcervical retrieval of fetal cells in the practice of modern medicine: a review of the current literature and future direction

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