FISH analysis of telomeric repeat sequences and their involvement in chromosomal aberrations induced by radiomimetic compounds in hamster cells

Bolzán, Alejandro D.; Pàez, Gerardo L.; Bianchi, Martha S.

doi:10.1016/s0027-5107(01)00162-2

Cited by 32 publications

(57 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As expected from our previous work [Bolzán et al, 2001;Bianchi, 2004a,b, 2005;Bolzán, unpublished data], the use of a telomeric PNA probe resulted in the detection of all telomeres present in the metaphase spreads of CHE (Fig. 1) and CPC (Fig.…”

Section: Resultssupporting

confidence: 82%

“…These cells were chosen because they exhibit strong and easily identified telomeric signals after telomere FISH [Bolzán et al, 2001;Bianchi, 2004a,b, 2005;Bolzán, unpublished]. They also possess chromosomes whose centromeres are clearly distinguishable using DAPI staining, which allows a very accurate determination of centric and acentric ICE.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A comparative analysis of bleomycin‐induced incomplete chromosome elements in two mammalian cell lines using a telomeric PNA probe

Flaqué

Bianchi

Bolzán

2006

Environ and Mol Mutagen

Self Cite

View full text Add to dashboard Cite

Fluorescence in situ hybridization (FISH) with a telomeric peptide nucleic acid probe was employed to analyze the induction of incomplete chromosome elements (ICE; i.e., incomplete chromosomes and terminal fragments) by bleomycin (BLM) in two mammalian cell lines. Chinese hamster embryo cells (CHE cell line, average 2n = 23) and domestic rabbit cells (CPC cell line, average 2n = 44) were treated with 2.5 micro g/ml BLM; after 18 hr of incubation, first-division metaphases were stained with the telomeric probe, and ICE and other unstable chromosomal aberrations were scored. BLM induced ICE, dicentrics, and interstitial acentric fragments in CHE cells, but only ICE in CPC cells. About 50% of the metaphases in BLM-treated CHE cells contained one or more pairs of ICE, while only 20% of treated CPC cells contained ICE. Almost 100% of the BLM-induced ICE in both cell lines consisted of pairs formed by an incomplete chromosome and a terminal fragment. Our results confirm that ICE are the most frequent type of unstable chromosomal aberration induced by BLM in mammalian cells. Moreover, the present study shows that an increase in the chromosome number does not necessarily result in an increase in the frequency of BLM-induced ICE. The results also show that the difference in the chromosomal sensitivity to BLM in CHE and CPC cells is due to differences in the absolute frequency but not in the pattern (i.e., type and proportion) of ICE.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

A comparative analysis of bleomycin‐induced incomplete chromosome elements in two mammalian cell lines using a telomeric PNA probe

Flaqué

Bianchi

Bolzán

2006

Environ and Mol Mutagen

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has also been used to contribute to clinical prediction (21) and the cytogenetic investigation of various diseases (22,23). Another important application of this technique is the assessment of genotoxic risk immediately after exposure to various reagents (24), and it has also proven useful for investigating chromosomal damage immediately after irradiation, as well as the kinetics of CA formation (25). The PCC technique has been shown to be compatible with a number of chromosome staining techniques, including C-banding, centromere detection by FISH, and chromosome painting (7,8,(26)(27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

M’Kacher

Maalouf

Terzoudi

et al. 2015

International Journal of Radiation Oncology*Biology*Physics

View full text Add to dashboard Cite

The introduction of TC staining to the PCC fusion technique has made possible the rapid scoring of unstable CAs, including dicentrics, with a level of accuracy and ease not previously possible. This new approach can be used for biological dosimetry in radiation emergency medicine, where the rapid and accurate detection of dicentrics is a high priority using automated scoring. Because there is no culture time, this new approach can also be used for the follow-up of patients treated by genotoxic therapy, creating the possibility to perform the estimation of induced chromosomal aberrations immediately after the blood draw.

show abstract

“…Chinese hamster cells have been used as a general model system for the study of chromosomal aberrations involving hetITSs. These sequences are frequently involved in spontaneous (Bertoni et al, 1996) and radiation (Alvarez et al, 1993;Slijepcevic et al, 1996;Day et al, 1998), chemically (Fernandez et al, 1995;Bolzán et al, 2001) or restriction enzyme (Balajee et al, 1994) induced chromosomal aberrations. In fact, the frequency of aberrations involving these sequences is higher than expected based on the percentage of the genome covered by telomeric repeats.…”

Section: Instability and Functionmentioning

confidence: 99%

Telomeric repeats far from the ends: mechanisms of origin and role in evolution

Ruiz‐Herrera

Nergadze

Santagostino

et al. 2008

Cytogenet Genome Res

194

230

View full text Add to dashboard Cite

In addition to their location at terminal positions, telomeric-like repeats are also present at internal sites of the chromosomes (intrachromosomal or interstitial telomeric sequences, ITSs). According to their sequence organization and genomic location, two different kinds of ITSs can be identified: (1) heterochromatic ITSs (het-ITSs), large (up to hundreds of kb) stretches of telomeric-like DNA localized mainly at centromeres, and (2) short ITSs (s-ITSs), short stretches of telomeric hexamers distributed at internal sites of the chromosomes. Het-ITSs have been only described in some vertebrate species and they probably represent the remnants of evolutionary chromosomal rearrangements. On the contrary, s-ITSs are probably present in all mammalian genomes although they have been described in detail only in some completely sequenced genomes. Sequence database analysis revealed the presence of 83, 79, 244 and 250 such s-ITSs in the human, chimpanzee, mouse and rat genomes, respectively. Analysis of the flanking sequences suggested that s-ITSs were inserted during the repair of DNA double-strand breaks that occurred in the course of evolution. An extensive comparative analysis of the s-ITS loci and their orthologous ‘empty’ loci confirmed this hypothesis and suggested that the repair event involved the direct action of telomerase. Whereas het-ITSs seem to be intrinsically prone to breakage, the instability of s-ITSs is more controversial. This observation is consistent with the hypothesis that s-ITSs are probably not themselves prone to breakage but represent ‘scars’ of ancient breakage that may have occurred within fragile regions. This study will review the current knowledge on these two types of ITS, their molecular organization, how they arose during evolution, their implications for chromosomal instability and their potential applications as phylogenetic/forensic markers.

show abstract

FISH analysis of telomeric repeat sequences and their involvement in chromosomal aberrations induced by radiomimetic compounds in hamster cells

Cited by 32 publications

References 40 publications

A comparative analysis of bleomycin‐induced incomplete chromosome elements in two mammalian cell lines using a telomeric PNA probe

A comparative analysis of bleomycin‐induced incomplete chromosome elements in two mammalian cell lines using a telomeric PNA probe

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

Telomeric repeats far from the ends: mechanisms of origin and role in evolution

Contact Info

Product

Resources

About