Fish as bio indicators to determine the effects of pollution in river by using the micronucleus and alkaline single cell gel electrophoresis assay

Ali, Daoud; Almarzoug, Mohammed H. A.; Ali, Hussain; Samdani,; Hussain, Syed Danish; Manohrdas, S.

doi:10.1016/j.jksus.2020.07.012

Cited by 26 publications

(15 citation statements)

References 23 publications

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“…The micronuclei frequency increased progressively with the increase in the concentration of Zn, Cu, Pb, and Cd in Nile tilapia ( 14 ). Our results of the MN test about genotoxicity, water quality, the health status of aquatic animals, and their potential health risks to humans were consistent with previously published data ( 14 , 72 , 76 ). Similar findings have been reported in previous studies, such as the frequency of MN biosynthesis being highest in European minnow ( Phoxinus phoxinus ), eel ( Anguilla anguilla ) ( 77 ), Nile tilapia ( 78 ), crucian carp ( 79 ), and brown trout ( Salmo trutta ) ( 80 ).…”

Section: Discussionsupporting

confidence: 92%

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

et al. 2022

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The toxic waste and pollutants of heavy metals continuously pollute freshwater aquatic reservoirs, which have severe implications on aquatic life and human health. The present work aims to evaluate trace elements (Zn, Mn, Cu, Cd, and Pb) along with three sites, Mariout Lake, Abbassa, and River Nile Aswan in Egypt, using Nile tilapia (Oreochromis niloticus) as bioindicator. The quality assurance, health-risk assessment, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), quantitative polymerase chain reaction (qPCR), and micronucleus test were performed to investigate the effect of different trace elements on Hsp70 gene level and micronuclei formation. We observed the highest expression of Hsp70 protein band of 70 KD and stress-responsive Hsp70 gene in the liver followed by gills of Nile tilapia caught from Mariout and Abbassa, but the lowest expression was in Nile tilapia caught from Aswan. Obvious micronuclei were observed under the microscope in erythrocytes, and their number was gradually decreased in the following manner: Mariout > Abbassa > Aswan. Noticeably, Cu, Zn, and Mn contents were low. Still, Pb and Cd contents were higher than the toxicity level recommended by the Food and Agriculture Organization (FAO), The World Health Organization (WHO), and the European Commission (EC). These results showed that Hsp70's appearance at the two levels of mRNA and protein is an effective indicator for aquatic pollution besides the aberration at the chromosome level represented in the micronucleus test. Furthermore, these results showed that Nile tilapia of the Aswan region had comparatively low trace elements contamination and were suitable for consumption.

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Section: Discussionsupporting

confidence: 92%

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

et al. 2022

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“…ROS can be detoxified by cellular antioxidant defence systems, both enzymatic and non-enzymatic [15] while measuring the total antioxidant capacity (TAC), evaluating the overall efficacy of cellular antioxidants against oxyradicals, and providing a comprehensive assessment of responsiveness to oxidative stress [16]. DNA damage biomarkers can be used to assess the genotoxicity of environmental pollutants and a comet assay is a valuable tool for assessing genotoxicity in fish in the environment [17]. The induction of stress proteins is the only molecular mechanism used by fish to cope with stress [18].…”

Section: Introductionmentioning

confidence: 99%

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

et al. 2022

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Background Biomarkers have become a valuable tool in environmental assessment, since they contribute to predicting contaminants in monitoring programmes. This study aimed to investigate the toxicity of heavy metal pollution in Lake Qarun using a multibiomarker approach (morphological, oxidative stress, genotoxicity, stress proteins) in Solea aegyptiaca fish. During the winter and summer seasons, water and fish samples were collected from different locations along Lake Qarun; western and northern sectors were away from any source of pollution, while southern and eastern sectors were exposed to effluents discharged from El-Wadi and El-Bats drains, respectively. The environmental quality, as well as the accumulation of metals (Fe, Cu, Zn, Cd, Pb, Ni) in fish gills, liver, and muscles were assessed. Data were integrated using the integrated biomarker response index (IBRv2) for biomarker response interpretation. Results Water quality and bioaccumulation of heavy metals revealed a highly significant difference between samples collected from polluted sectors and those collected far from drainage water, and, seasonal differences were detected. Growth indices revealed a significant difference between sites and seasons. Fish from the western sector had the highest total antioxidant capacity in their gills, liver, and muscles, with no seasonal differences detected. However, the maximum value of malondialdehyde, protein carbonyl, 8-hydroxy-2′deoxyguanosine, metallothionein, heat shock protein 70, and DNA strand breaks in gills, liver, and muscles was detected in the polluting sectors. It revealed a significant difference between seasons, with the highest value during the winter season. According to IBRv2 results, the most effective biomarkers in this study were malondialdehyde and 8-hydroxy-2′deoxyguanosine in gills, protein carbonyl and metallothionein in the liver, heat shock protein 70 in gills and liver, and DNA strand break in gills, liver, and muscles. Conclusions This multibiomarker approach contributes to distinguishing between locations with varying levels of anthropogenic pollution, identifying the drainage water-exposed sectors as the most stressed and the winter season as the most critical time for Solea aegyptiaca owing to spawning. The biomarkers chosen are effective indicators in Solea aegyptiaca under stress, indicating the potential for environmental monitoring. Graphical Abstract

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“…However, since pesticides have become more widely used in agriculture in recent decades, environmental hazards and negative impacts on aquatic organisms have become a serious issue (Kayhan, et al, 2013;Huang, et al, 2019). Pesticides have a deleterious influence on environmental biodiversity and may cause physiological and stress impacts on non-target species (Elsharkawy, 2020), aquatic biotas, and human and animal health (Ali, et al, 2020). Aquatic creatures, such as fish, are in direct contact with pesticides dissolved in the water, making them more vulnerable to pesticides (Covantes-Rosales, et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparative Acute Exposure Study of Abamectin Different Formulations Inducing Physiological and Oxidative Stress Biomarkers in Nile Tilapia, (Oreochromis niloticus).

Ahmed

Reda

2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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The goal of this study is to assess the acute and sublethal potential hazards on tilapia fish (Oreochromis niloticus) exposed to two different commercial formulations of abamectin (ABM, 5%): emulsifiable concentration (EC) and microemulsions (ME). The median lethal concentration (LC50 -96 h) to each formulation was determined, as well as the adverse effects of sublethal concentrations (½ LC50) on physiological and oxidative stress biomarkers after 96hs of exposure. The LC50 (96 h) values for EC and ME were 10 and 16.6 µg/L, respectively. Furthermore, in both EC and ME, the findings of physiological and oxidative stress aspects revealed significant markedly increases in serum total protein, albumin, globulin, and urea, as well as a significant decline in aminotransferases activity (ALT and AST), and creatinine levels (Cre). Moreover, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione peroxidase (GPx) were greatly increased, whereas glutathione-S-transferase (GST) and glutathione (GSH) were significantly reduced in the liver and brain. MATERIALS AND METHODS Pesticide Used:Abamectin (ABM) as two formulations: 5% EC (Profery) and 5% ME (Spider gold) were supplied by the central agricultural pesticide laboratory (CAPL), Agricultural

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Fish as bio indicators to determine the effects of pollution in river by using the micronucleus and alkaline single cell gel electrophoresis assay

Cited by 26 publications

References 23 publications

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

Comparative Acute Exposure Study of Abamectin Different Formulations Inducing Physiological and Oxidative Stress Biomarkers in Nile Tilapia, (Oreochromis niloticus).

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