Fish Cell Culture: Properties of a Cell Line from the Sheepshead, Archosargus probatocephalus

Abstract

A cell line designated SHF-1 was established from fin tissue of the sheepshead, Archosargus probatocephalus. Cells were fibroblastlike and grew best at 26 °C in Leibovitz (L-15) medium containing serum supplements and a final sodium chloride concentration of 0.150 M. The 2N number of primary sheepshead cells was 48 but the SHF-1 line at passage levels above 11 was heteroploid. SHF-1 cells did not replicate any of seven viruses tested. Species confirmation of SHF-1 was accomplished by the cytotoxic antibody test and was attempted with isoelectric focusing of lactic dehydrogenase isoenzymes. Plating efficiency and freeze viability studies were also performed. Key words: fish cell culture, sheepshead, Archosargus probatocephalus, microbiology

Cytogenetical studies on fishes, IV. Karyotypes of six species in the sparoid fishes.

“…This species would be classified into the first group categorized by us. Another sparoid species, Aechosargus probatocephalus, from Gulf of Mexico also had the same diploid chromosome number as the present materials (Law et al 1978) . The karyotype of this species was not corresponded with anyone of the above all seven sparoid species, because five biarmed chromosomes (one unpaired metacentric and two pairs of submetacentric chromosomes) and forty-three acrocentric chromosomes were included.…”

“…Biochemical polymorphism in sparoid fishes has been studied in Pagrus major (Taniguchi and Okada 1980) collected from near the coast of Japan, and in Chrysophrys auratus (Smith et al 1978) from near the coast of New Zealand. The karyotypes of three sparoid species have already been reported by Nishikawa and Karasawa (1972); Law et al (1978) andVasiliev (1978). In the present paper the karyotype of six sparoid fish species including one species (Pagrus major) published by Nishikawa and Karasawa (1972) will be described with special regard to the relationship between the karyotype evolution and species differentiation in this group.…”

Development of two bone-derived cell lines from the marine teleost Sparus aurata ; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin

“…In contrast, when cells were grown at 33C in D-MEM, a medium well suited for supporting growth of a broad spectrum of mammalian cell lines, including bone-derived cells (Cooper et al 1998;Solomon et al 2000), and also previously used to maintain some fish cell lines (Bejar et al 1997;Lehane et al 1999), both cell types exhibited a polygonal phenotype and a rapid growth rate and were able to mineralize their ECM. The two cell types were able to adapt to readily available standard media without special requirements, such as the addition of NaCl, a supplement needed to sustain the growth of some marine fish cells (Clem et al 1961;Law et al 1978;Li et al 1985). Changing the culture medium brought a change in phenotype, growth performance, and mineralizing ability of these cells, indicating the presence of factors in D-MEM necessary for cell differentiation and its capability for sustaining in vitro mineralization in fish cells as previously seen for mammalian cells.…”

Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer)

“…The SISK cell line has been maintained through more than 100 passages over a 2-year period. This cell line has good adaptation for growth in Leibovitz' L-15 without special requirements, such as NaCl addition which is needed for other marine fish cells (Clem et al, 1961;Law et al, 1978;Li et al, 1984;Fernandez et al, 1993).…”