Fish Collagen Hydrolysates Modulate Cartilage Metabolism

Abstract

Osteoarthritis is a degenerative joint disease in which interleukin-1β plays a major role in the inflammatory process. Administration of collagen hydrolysate was an optional treatment of osteoarthritis. Fish has become an interesting source of collagen hydrolysate because of religious reason and there is no risk from mad cow disease. However, the effects of different sizes of fish collagen hydrolysate on cartilage and chondrocyte metabolism have not been well studied yet. This study examined the effect of different sizes of fish collagen hydrolysate on cartilage metabolism. Three different sizes of fish collagen hydrolysate were prepared by size exclusion using centrifugation, which composed of small fraction (<3 kDa), medium fraction (3-10 kDa) and large fraction (>10 kDa). Using porcine cartilage explant, in physiological condition, all the three fractions had no effect on cartilage metabolism, but they could induce pro-MMP3 and pro-MMP13 secretions through activation of p-ERK and p-p38. In pathological condition induced by interleukin-1β and oncostatin-M, small and medium fractions showed additive effect with interleukin-1β and oncostatin-M on cartilage degradation, whereas large size had no effect. In addition, the effect of small size occurred through further activation of p-p65, which resulted in further induction of active-MMP13, while medium size had a different mechanism. In conclusion, all three fractions fish collagen hydrolysate had no effect on cartilage metabolism in physiological condition, but small and medium fractions had adverse effect on cartilage in pathological condition. Taken together, various sizes of fish collagen hydrolysate showed different effects on cartilage metabolism. Therefore, different sizes of fish collagen hydrolysates play different roles on cartilage metabolism, especially in the pathological condition.

High Throughput Identification of Antihypertensive Peptides from Fish Proteome Datasets

“…This gives rise to the repeat occurrence of AHTPs of GLP and LPG. Furthermore, their bioactivities demonstrate their remarkable advantages in various fields [ 42 , 43 ]. For example, Vastatin, an endogenous antiangiogenic polypeptide from non-collagenous C-terminal globular domain of col8a1, has been reported to be promising in fighting against glioblastoma [ 44 ].…”

Role of Collagen Derivatives in Osteoarthritis and Cartilage Repair: A Systematic Scoping Review With Evidence Mapping

“…These exciting results have, however, been moderated by findings from two in vitro studies conducted by the same research group from Germany, which compared different CH preparations (different sources and sizes) and concluded that CHs from various sources differed in terms of their peptide composition and were either ineffective or even detrimental to OA cartilage [43,44]. Similar findings were reported by a subsequent study from Thailand, published in 2018, which compared fish CH of different molecular weights [41]. Besides the results from these in vitro studies, the findings from the in vivo studies have been confirmed by clinical studies in OA patients.…”

“…Types of Studies A total of 41 studies have been published on collagen derivatives in OA and cartilage repair so far. Sixteen of these studies were preclinical in vitro and in vivo studies [25,26,[41][42][43][44][45][46][47][48][49][50][51][52][53][54] and 25 were clinical studies (including clinical trials, observational studies, and systematic reviews/ meta-analyses) [27,28,31,. Figure 2 depicts the distribution of the studies by specific study type.…”

“…Cartilage metabolism and degradation outcomes, as well as levels of various inflammatory mediators, were measured. Three of these studies, assessing different preparations of CH, from different sources and with different molecular weights, found that CH was either ineffective or detrimental to articular cartilage in pathological condition [41,43,44], suggesting that collagen preparations from various sources might have different peptide composition and differ significantly in their effects on articular cartilage (see conclusions in Table 1). All of these studies advocated for further research in this setting.…”

Osteoarthritis‐alleviating effects in papain‐induced model rats of chicken cartilage hydrolysate and its peptide fractions

“…HC is also a therapeutic agent that has been used for long‐term treatment of OA owing to HC's high safety (Oesser et al ., ). At the same time, HC had no effect on cartilage metabolism (Boonmaleerat et al ., ). Guillerminet et al .…”