Fish IKKα from Japanese eel (Anguilla japonica) can activate NF-κB, AP1, and type I IFN signaling pathways

Feng, Jia; Xu, Yuankai; Lin, Peng; Wang, Yilei; Zhang, Ziping; Zou, Pengfei; Peng, Xinwei

doi:10.1016/j.fsi.2020.09.012

Cited by 9 publications

(9 citation statements)

References 39 publications

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“…and tumour necrosis factorα (TNFα) in orange-spotted grouper (Epinephelus coioides) (Feng et al, 2020). In our work, the gene expression of IKKα in HP versus C group was obviously downregulated (Figure 5), but in HB versus C group some immune factors like IL-6 and TNFα were no significant changed trend.…”

Section: Nf-κb Signalling Pathwaymentioning

confidence: 50%

“…IKKα has been identified, cloned and characterized in several teleost fishes (He et al., 2020; Qu et al., 2015). Previous studies indicated that over‐expressed IKKα significantly increased the expression of proinflammatory cytokines interleukin −6 ( IL‐6 ) and tumour necrosis factor‐α ( TNF‐α ) in orange‐spotted grouper ( Epinephelus coioides ) (Feng et al, 2020). In our work, the gene expression of IKKα in HP versus C group was obviously down‐regulated (Figure 5), but in HB versus C group some immune factors like IL‐6 and TNF‐α were no significant changed trend.…”

Section: Discussionmentioning

confidence: 99%

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Administration of a recombinant ALDH7A1 (rA7) indicates potential regulation of the metabolite and immunology pathways in Atlantic salmon infected with Aeromonas salmonicida

Fan

Liu

et al. 2021

Journal of Fish Diseases

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The bacterium Aeromonas salmonicida is the pathogen responsible for furunculosis, which is a serious disease of salmonids. This disease has a significant economic impact on the economic benefits of the global salmon farming industry. However, the pathogenesis of this disease in fish is still unknown. Members of the aldehyde dehydrogenase gene (ALDH) superfamily play a key role in the enzyme detoxification of endogenous and exogenous aldehydes. In this study, we obtained a recombinant aldehyde dehydrogenase 7A1 (ALDH7A1) protein to find its functions on Atlantic salmon infected by A. salmonicida. The transcriptional response in the liver of Atlantic salmon (Salmo salar) with differing levels of A. salmonicida infection was analysed and compared in order to reveal mechanisms by which ALDH7A1 may confer infection resistance. With the addition of ALDH7A1 protein, it was found that a total of 13,369 genes were annotated with one or more KEGG and localized to 360 KEGG pathways in the high concentration infection group. The differential expression genes were more enriched in immune signalling pathways such as the Toll‐like receptor signalling pathway, NF‐kappa B signalling pathway and TNF signalling pathway. On the other hand, at low concentrations of infection, KEGG enriched a smaller number of differential expression genes. However, these differential genes were more concentrated in immune signalling pathways such as the PI3K‐Akt signalling pathway, JAK‐STAT signalling pathway and complement and coagulation cascades. In addition, several known immune‐related genes including HSP90α, HSP70, DNA damage‐inducible transcript 4, integrin alpha 5 and microtubule‐associated protein 2 were among the differentially expressed transcripts. These data provide the first insights into the host–ALDH7A1 vaccine interactome. The results of this study contribute to identifying the potential resistance mechanisms of Atlantic salmon to A. salmonicida infection and determining future treatment strategies.

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Section: Nf-κb Signalling Pathwaymentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Administration of a recombinant ALDH7A1 (rA7) indicates potential regulation of the metabolite and immunology pathways in Atlantic salmon infected with Aeromonas salmonicida

Fan

Liu

et al. 2021

Journal of Fish Diseases

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“…Fuqing eel farms provided healthy Japanese eels (weighing 40-45 g), which were temporarily raised in circulating water for two weeks. Different tissues were collected under natural conditions and tissue RNA was extracted to detect tissue distribution as previously studied [27]. The procedure for in vivo and in vitro immunostimulation experiments is described in a previous report from our laboratory [27].…”

Section: Fish Tissue Collection and Immune Challengementioning

confidence: 99%

“…Different tissues were collected under natural conditions and tissue RNA was extracted to detect tissue distribution as previously studied [27]. The procedure for in vivo and in vitro immunostimulation experiments is described in a previous report from our laboratory [27]. Tryptone soy broth (TSB) was infected with Aeromonas hydrophila, and the mixture was shaken at 28 • C for 24 h. The bacteria were collected and diluted in 0.01 mmol/L PBS (pH 7.4) to a concentration of 4 × 10 4 cfu/mL.…”

Section: Fish Tissue Collection and Immune Challengementioning

confidence: 99%

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CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Wang,

Lin,

Wang

et al. 2023

Animals

Self Cite

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IFNAR1, one of the type I IFN receptors, is crucial to mammalian host defense against viral invasion. However, largely unknown is the immunological role of the fish teleost protein IFNAR1, also known as CRFB5. We have successfully cloned the whole cDNA of the Japanese eel’s (Anguilla japonica) CRFB5a homolog, AjCRFB5a. The two fibronectin-3 domains and the transmembrane region (238–260 aa) of AjCRFB5a are normally present, and it shares a three-dimensional structure with zebrafish, Asian arowana, and humans. According to expression analyses, AjCRFB5a is highly expressed in all tissues found, particularly the liver and intestine. In vivo, Aeromonas hydrophila, LPS, and the viral mimic poly I:C all dramatically increased AjCRFB5a expression in the liver. Japanese eel liver cells were reported to express AjCRFB5a more strongly in vitro after being exposed to Aeromonas hydrophila or being stimulated with poly I: C. The membranes of Japanese eel liver cells contained EGFP-AjCRFB5a proteins, some of which were condensed, according to the results of fluorescence microscopy. Luciferase reporter assays showed that AjCRFB5a overexpression strongly increased the expression of immune-related genes in Japanese eel liver cells, such as IFN1, IFN2, IFN3, IFN4, IRF3, IRF5, and IRF7 of the type I IFN signaling pathway, as well as one of the essential antimicrobial peptides LEAP2, in addition to significantly inducing human IFN-promoter activities in HEK293 cells. Additionally, RNA interference (RNAi) data demonstrated that knocking down AjCRFB5a caused all eight of those genes to drastically lower their expression in Japanese eel liver cells, as well as to variable degrees in the kidney, spleen, liver, and intestine. Our findings together showed that AjCRFB5a participates in the host immune response to bacterial infection by inducing antimicrobial peptides mediated by LEAP2 and favorably modulates host antiviral immune responses by activating IRF3 and IRF7-driven type I IFN signaling pathways.

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A new subtype of type I IFN receptor, CRFB5b, plays an active role in host antiviral and antibacterial functions in Japanese eel (Anguilla japonica)

Wang,

Lin,

Wang

et al. 2023

Aquacult Int

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Fish IKKα from Japanese eel (Anguilla japonica) can activate NF-κB, AP1, and type I IFN signaling pathways

Cited by 9 publications

References 39 publications

Administration of a recombinant ALDH7A1 (rA7) indicates potential regulation of the metabolite and immunology pathways in Atlantic salmon infected with Aeromonas salmonicida

Administration of a recombinant ALDH7A1 (rA7) indicates potential regulation of the metabolite and immunology pathways in Atlantic salmon infected with Aeromonas salmonicida

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

A new subtype of type I IFN receptor, CRFB5b, plays an active role in host antiviral and antibacterial functions in Japanese eel (Anguilla japonica)

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