FISH—in Fish Chromosomes

Giant Sex Chromosomes in Omophoita Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)

“…In all experiments, the final probe cocktail for each experiment was composed of 500 ng of each probe and 15 µg of C0t-1 DNA of the respective individuals, diluted in the same hybridization buffer as described above. FISH experiments were performed using high-stringency conditions in accordance with Sassi et al [ 39 ]. In summary, prior to the hybridization, the slides were aged for two hours at 37 °C, followed by RNAse A (90 min, 37 °C) and pepsin (50 g/mL in 10 mM HCl, 3 min, 37 °C) treatments.…”

Chromosomal Rearrangements and Satellite DNAs: Extensive Chromosome Reshuffling and the Evolution of Neo-Sex Chromosomes in the Genus Pyrrhulina (Teleostei; Characiformes)

“…We also used whole-chromosome painting (WCP) using a Y-specific probe (Psemi-Y) previously obtained [ 29 ] to detect the sex chromosomes of P. semifasciata and the proto-sex pairs in P. brevis and P. obermulleri . For this, sequential FISH/WCP was performed following [ 99 ]. In total, 10 PseSatDNAs showed visible FISH signals in P. semifasciata, and 07 PmaSatDNA showed a visible FISH signal in P. marilynae .…”

A comparative cytogenetic study of Hypsibarbus malcolmi and H. wetmorei (Cyprinidae, Poropuntiini)

“…These sequences were directly labeled by Cy3 at the 5'end during synthesis (Sigma, St. Louis, MO, USA) as described by Kubat et al (2008) . FISH was performed under stringent conditions and hybridization occurred overnight in a moist chamber at 37 °C ( Sassi et al 2023 ). Chromosomes were counterstained with 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI, 1.2 μg/ml) mounted in antifade solution (Vector, Burlingame, CA, USA,).…”