Fish oil attenuates persistent inflammatory pain in rats through modulation of TNF-α and resolvins

Lobo, Bianca Waruar; Lima, Cleverton Kleiton Freitas de; Teixeira, Monique S.; Silva, Natália L. C.; Takiya, Christina Maeda; Ramos, Mônica Freiman de Souza; Miranda, Ana Luísa P.; Dellamora-Ortiz, Gisela Maria

doi:10.1016/j.lfs.2016.03.034

Cited by 24 publications

(19 citation statements)

References 36 publications

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“…Concentrate fish oil, containing 4.6% EPA and 3.6% DHA, was obtained commercially (OMACOR ® , Pierre Fabre). Based on previous studies, two doses were selected 2.3 g/kg and 4.6 g/kg of EPA content, respectively 1.9 and 3.8 g of DHA/kg ( Lobo et al, 2016 ). Two groups of animals received daily oral treatment, one for 10 days starting at the day of the surgery (preventive protocol) and another for 5 days starting 5 days after nerve surgery (therapeutic protocol).…”

Section: Methodsmentioning

confidence: 99%

“…The benefits of concentrate fish oil (CFO) (84% of EPA and DHA ethyl esters) in peripheral NP have not been investigated yet, neither its ability to prevent NP development. In a previous study from our group CFO displayed superior inhibition of inflammatory pain when compared to FO in rodents because of its increased composition in EPA and DHA ( Lobo et al, 2016 ). In this context, this study aimed to evaluate the effect of CFO oral treatment in preventing NP development after partial sciatic nerve ligation (PSNL) in mice, shedding light on its mechanism of action.…”

Section: Introductionmentioning

confidence: 98%

“…Fish oil (FO), which is the main source of EPA and DHA, have exhibited analgesic and anti-inflammatory activities in animal models of chronic pain ( Veigas et al, 2011 ; Lobo et al, 2016 ). The exact mechanisms underlying the effect of ω-3 PUFAs on reducing pain sensitivity is not fully elucidated ( Trépanier et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…The exact mechanisms underlying the effect of ω-3 PUFAs on reducing pain sensitivity is not fully elucidated ( Trépanier et al, 2016 ). However, animal studies have attributed PUFAs benefits to modulation of neuronal activity, regulation of immune cells response and also production of more potent pro-resolving and anti-inflammatory lipid mediators, such as resolvins ( Lu et al, 2013 ; Heng et al, 2015 ; Lobo et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

et al. 2017

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Fish oil (FO) is the main source of long chain omega-3 polyunsaturated fatty acids (ω-3 PUFAs), which display relevant analgesic and anti-inflammatory properties. Peripheral nerve injury is driven by degeneration, neuroinflammation, and neuronal plasticity which results in neuropathic pain (NP) symptoms such as allodynia and hyperalgesia. We tested the preventive effect of an EPA/DHA-concentrate fish oil (CFO) on NP development and regenerative features. Swiss mice received daily oral treatment with CFO 4.6 or 2.3 g/kg for 10 days after NP was induced by partial sciatic nerve ligation. Mechanical allodynia and thermal hypernociception were assessed 5 days after injury. CFO 2.3 g/kg significantly prevented mechanical and thermal sensitization, reduced TNF levels in the spinal cord, sciatic MPO activity, and ATF-3 expression on DRG cells. CFO improved Sciatic Functional Index (SFI) as well as electrophysiological recordings, corroborating the increased GAP43 expression and total number of myelinated fibers observed in sciatic nerve. No locomotor activity impairment was observed in CFO treated groups. These results point to the regenerative and possibly protective properties of a combined EPA and DHA oral administration after peripheral nerve injury, as well as its anti-neuroinflammatory activity, evidencing ω-3 PUFAs promising therapeutic outcomes for NP treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

et al. 2017

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“…These in turn regulate several downstream targets, some that are involved in acute central sensitization that induces persistent hyperalgesia, and others that act through microRNAs to alter transcription and effect a more long-lasting genomic response. The rapid responders include transient receptor potential (TRP) channels 3,43,44 , TNF-α activity 1,33,39 , mitogen-activated protein kinase (MAPKinase) activation, particularly p38 and ERK and jnk phosphorylation 64,65 , and NMDA receptors. 16 The slower responses that are longer lasting include changes in microRNAs that regulate receptor and ligand expression in inflammatory cells 35,36 and are likely to also participate in such regulation in neurons and glia.…”

Section: Discussionmentioning

confidence: 99%

Prevention of Chronic Post-Thoracotomy Pain in Rats By Intrathecal Resolvin D1 and D2: Effectiveness of Perioperative and Delayed Drug Delivery

Wang

Strichartz

2017

The Journal of Pain

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Thoracotomy results in a high frequency of chronic post-operative pain. Resolvins are endogenous molecules, synthesized and released by activated immune cells, effective against inflammatory and neuropathic pain. Different resolvins have differential actions on selective neuronal and glial receptors and enzymes. This paper examines the ability of intrathecal Resolvin D1 (RvD1) and Resolvin D2 (RvD2) to reduce chronic postthoracotomy pain in rats. Thoracotomy, involving intercostal incision and rib retraction, resulted in a decrease in the mechanical force threshold to induce nocifensive behavior, an enlargement of the pain-sensitive area, and an increase in the fraction of rats showing nocifensive behavior, all for at least 5 weeks. The qualitative nature of the behavioral responses to tactile stimulation changed dramatically after thoracotomy, including the appearance of vigorous behaviors, such as turning, shuddering, and squealing, all absent in naive rats. Intrathecal delivery of RvD1 (30ng/30µL), at surgery or 4 days later, halved the spread of the mechano-sensitive area, lowered by 60% the percent of rats with tactile hypersensitivity, and reduced the drop in threshold for a nocifensive response, along with a reduction in the occurrence of vigorous nocifensive responses. RvD2’s actions on threshold changes were statistically the same. These findings suggest that intrathecal resolvins, delivered pre-operatively or several days later, can prevent chronic post-operative hyperalgesia.

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Protective Effect of Omega 3 Fatty Acids EPA and DHA in the Neurodegenerative Disease

Leo¹,

Herrera²,

Campos³

2018

Reference Series in Phytochemistry

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Fish oil attenuates persistent inflammatory pain in rats through modulation of TNF-α and resolvins

Cited by 24 publications

References 36 publications

Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice

Prevention of Chronic Post-Thoracotomy Pain in Rats By Intrathecal Resolvin D1 and D2: Effectiveness of Perioperative and Delayed Drug Delivery

Protective Effect of Omega 3 Fatty Acids EPA and DHA in the Neurodegenerative Disease

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