Fish oil supplementation attenuates changes in plasma lipids caused by dexamethasone treatment in rats

Barbosa, Amanda Marreiro; Francisco, Priscila de C.; Motta, Katia; Chagas, Thayz Rodrigues; Santos, Cristiane dos; Rafacho, Alex; Nunes, Everson Araújo

doi:10.1139/apnm-2015-0487

Cited by 20 publications

(15 citation statements)

References 50 publications

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“…4-VCD treatment exerted no major effects on the glycerol release from the perigonadal adipose tissue which was in accordance to their absent effect on the circulating triacylglycerol levels. The absence of significant differences between basal and isoproterenol-stimulated glycerol release in rats treated with dexamethasone is in agreement with previous studies showing similar results [34,50] and may be associated to reduced lipoprotein lipase activity [51] and augmented hormone-sensitive lipase activity [52].…”

Section: Discussionsupporting

confidence: 92%

Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment

Battiston

Santos

Barbosa

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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4-vinilcyclohexene diepoxide (4-VCD) causes premature ovarian failure and may result in estrogen deficiency, characterizing the transition to estropause in rodents (equivalent to menopause in women). Estropause/menopause is associated with metabolic derangements such as glucose intolerance and insulin resistance. Glucocorticoids (GCs) are known to exert diabetogenic effects. Thus, we aimed to investigate whether rats with premature ovarian failure are more prone to the diabetogenic effects of GC. For this, immature female rats received daily injections of 4-VCD [160mg/kg body weight (b.w.), intraperitoneally (i.p.)] for 15 consecutive days, whereas control rats received vehicle. After 168days of the completion of 4-VCD administration, rats were divided into 4 groups: CTL-received daily injections of saline (1mL/kg, b.w., i.p.) for 5days; DEX-received daily injections of dexamethasone (1mg/kg, b.w., i.p.) for 5days; VCD-treated as CTL group; VCD+DEX-treated as DEX group. Experiments and euthanasia occurred one day after the last dexamethasone injection. 4-VCD-treated rats exhibited ovary hypotrophy and reduced number of preantral follicles (p<0.05). Premature ovarian failure had no impact on the body weight gain or food intake, but both were reduced by the effects of dexamethasone. The increase in blood glucose, plasma insulin and triacylglycerol levels as well as the reduction in insulin sensitivity caused by dexamethasone treatment was not exacerbated in the VCD+DEX group of rats. Premature ovarian failure did change neither the hepatic content of glycogen and triacylglycerol nor the glycerol release from perigonadal adipose tissue. Glucose intolerance was observed in the VCD group after an ipGTT (p<0.05), but not after an oral glucose challenge. Glucose intolerance and compensatory pancreatic β-cell mass caused by GC were not modified by ovarian failure in the VCD+DEX group. We conclude that reduced ovarian function has no major implications on the diabetogenic effects promoted by GC treatment, indicating that other factors related to aging may make rats more vulnerable to GC side effects on glucose metabolism.

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Section: Discussionsupporting

confidence: 92%

Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment

Battiston

Santos

Barbosa

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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“…), between 0800 and 0900 h, for 33 consecutive days. The dose of fish oil was based on previous publications using rats as an experimental model [ 24 , 25 ]. The o.g.…”

Section: Methodsmentioning

confidence: 99%

Impact of Fish Oil Supplementation and Interruption of Fructose Ingestion on Glucose and Lipid Homeostasis of Rats Drinking Different Concentrations of Fructose

Sulis

Motta

Barbosa

et al. 2017

BioMed Research International

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Background. Continuous fructose consumption may cause elevation of circulating triacylglycerol. However, how much of this alteration is reverted after the removal of fructose intake is not known. We explored this question and compared the efficacy of this approach with fish oil supplementation. Methods. Male Wistar rats were divided into the following groups: control (C), fructose (F) (water intake with 10% or 30% fructose for 9 weeks), fish oil (FO), and fructose/fish oil (FFO). Fish oil was supplemented only for the last 33 days of fructose ingestion. Half of the F group remained for additional 8 weeks without fructose ingestion (FR). Results. Fructose ingestion reduced food intake to compensate for the increased energy obtained through water ingestion, independent of fructose concentration. Fish oil supplementation exerted no impact on these parameters, but the removal of fructose from water recovered both ingestion behaviors. Plasma triacylglycerol augmented significantly during the second and third weeks (both fructose groups). Fish oil supplementation did not attenuate the elevation in triacylglycerol caused by fructose intake, but the interruption of sugar consumption normalized this parameter. Conclusion. Elevation in triacylglyceridemia may be recovered by removing fructose from diet, suggesting that it is never too late to repair improper dietary habits.

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“…To determine effect of NSO on insulin resistance, an insulin tolerance test was performed as previously described (Woo et al, 2014;Barbosa et al, 2015). Briefly, 15 animals were randomized into 3 groups, with 5 animals per group: a normal saline group that received 1 ml/kg (0.9% NaCl), a control group receiving 1 mg/kg bodyweight of dexamethasone and a treated group receiving dexamethasone (1 mg/kg) and N. sativa (DEXNSO 0.4ml/kg) for 5 days.…”

Section: Animals Drugs and N Sativa Oilmentioning

confidence: 99%

Nigella sativa L. oil ameliorates insulin resistance caused by dexamethasone treatment in male Wistar rats

Alli-Oluwafuyi¹,

Amin²,

Abdulmajeed³

et al. 2017

Afr. J. Pharm. Pharmacol.

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Dexamethasone is an anti-inflammatory glucocorticoid that may alter glucose and lipid homeostasis depending on the dose and/or duration of administration. Nigella sativa oil (NSO) can be used as potential modulator of intermediary glucose and lipid metabolism. Herein, the authors evaluated the dose-dependent effects of NSO (0.2 and 0.4 ml/kg body weight) supplementation on plasma glucose, insulin and triglyceride concentration in rats treated with dexamethasone (1 mg/kg body weight) for 5 days. Adult male Wistar rats (150 to 200 g) were housed and treated in the animal holding facility of the Faculty of Basic Medical Sciences, University of Ilorin, Ilorin. Animals were randomly distributed into 4 groups: control (normal saline, 1 ml/kg), DEX (dexamethasone 1 mg/kg), DEX + NSO 0.2 ml/kg and DEX+ NSO 0.4 ml/kg. All drugs were administered intraperitoneally. To assess insulin resistance, an insulin tolerance test was performed in addition to fasting glucose and insulin measurements used in calculating the homeostatic model assessment of insulin resistance index (HOMA-IR). Insulin-induced glucose lowering was improved following NSO supplementation during the insulin tolerance test (p<0.05). Likewise, there was a reduction in fasting hyperinsulinemia (50%; p<0.05) and HOMA-IR index. Dexamethasone induced hypertriglyceridemia which was not significantly reduced following NSO supplementation. Furthermore, NSO had no significant effect on liver alanine aminotransferase enzyme activity in rats treated with dexamethasone. In conclusion, it is shown that 5 days therapy with dexamethasone can be used as a model of insulin resistance and NSO supplementation for 5 consecutive days attenuates hyperglycemia and insulin resistance induced by dexamethasone treatment.

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Fish oil supplementation attenuates changes in plasma lipids caused by dexamethasone treatment in rats

Cited by 20 publications

References 50 publications

Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment

Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment

Impact of Fish Oil Supplementation and Interruption of Fructose Ingestion on Glucose and Lipid Homeostasis of Rats Drinking Different Concentrations of Fructose

Nigella sativa L. oil ameliorates insulin resistance caused by dexamethasone treatment in male Wistar rats

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