Fish-on-a-chip: a sensitive detection microfluidic system for alzheimer's disease

Devadhasan, Jasmine Pramila; Kim, Sang-Hyo; An, Jeongho

doi:10.1186/1423-0127-18-33

Cited by 28 publications

(17 citation statements)

References 147 publications

(174 reference statements)

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“…9 Microuidic cell culture devices have been used in studies on drug metabolism and toxicity, 10 as a diagnostic tool for neurological disorder, 11 and for evaluating the inuence of different factors on cancer development and stem cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

et al. 2014

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A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.

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Section: Introductionmentioning

confidence: 99%

A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

et al. 2014

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“…3 Moreover, the CMOS image sensor chip was modified into an impedance sensor, 4 and used for microarray generation to detect DNA-DNA hybridization and also for the detection of antigen-antibody (Ag-Ab) interactions. 5,6 Even though the Ag-Ab interaction has taken place on CMOS image sensor chips, the final interaction has been confirmed by other devices, such as SEM, atomic force microscopy (AFM) and luminescence or fluorescence-based detections. 7,8 Our group has tried to develop a simple and highly sensitive diagnostic method based on the CMOS image sensor for the detection of Ag-Ab interactions on indium nanoparticle (InNP) substrates.…”

Section: Introductionmentioning

confidence: 99%

CMOS Image Sensor-based Immunodetection by Refractive-Index Change

Devadhasan

Kim

2012

ANAL. SCI.

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A complementary metal oxide semiconductor (CMOS) image sensor is an intriguing technology for the development of a novel biosensor. Indeed, the CMOS image sensor mechanism concerning the detection of the antigen-antibody (Ag-Ab) interaction at the nanoscale has been ambiguous so far. To understand the mechanism, more extensive research has been necessary to achieve point-of-care diagnostic devices. This research has demonstrated a CMOS image sensor-based analysis of cardiovascular disease markers, such as C-reactive protein (CRP) and troponin I, Ag-Ab interactions on indium nanoparticle (InNP) substrates by simple photon count variation. The developed sensor is feasible to detect proteins even at a fg/mL concentration under ordinary room light. Possible mechanisms, such as dielectric constant and refractive-index changes, have been studied and proposed. A dramatic change in the refractive index after protein adsorption on an InNP substrate was observed to be a predominant factor involved in CMOS image sensor-based immunoassay.

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“…Paraffin-embedded tissue slides require a pretreatment procedure before hybridization, and the samples are analyzed by fluorescence microscopy. Conventional FISH analysis is a time-consuming (takes 2 -3 days to complete), labor-intensive, and expensive (~$90 per slide 8 ) technique.…”

Section: Conventional Fish Proceduresmentioning

confidence: 99%

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

Sato

2015

ANAL. SCI.

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867 IntroductionCellular pathology techniques have progressed in recent years. Conventionally, the pathologist observes tissues with a microscope and determines whether or not the hematoxylin and eosin (H&E)-stained tissues contain cancer cells based on their morphology. The H&E stain is the traditional and most widely used staining method in medical diagnoses. 1 Recent advances in genetic analysis methods have made it possible to identify the genetic basis of human diseases, and have opened the door to individualized prevention strategies and early detection and treatment.Therefore, combinations of the traditional morphological diagnosis method and newer genetic methodologies are strongly welcomed.Fluorescence in situ hybridization (FISH) is a well-known gene-based method used to image genetic abnormalities. FISH is a microscopic technique in which specific DNA sequences tagged with fluorophores are used to detect target genes and identify their localization within a cell. FISH was developed in the early 1980s 2 and has since been improved continuously. 3 The FISH probes can only detect genes with a high degree of homology, and can visualize specific cytogenetic abnormalities such as copy number aberrations, gene mutations, and gene fusions. 4 This advanced molecular pathology technique has enabled better diagnoses of diseases, leading to more tailored therapeutic regimens.Analytes have become more multifaceted. Microfluidic devices enable the miniaturization, integration, automation, and parallelization of chemical and biochemical processes. This new technology also provides opportunity for expansion in the field of cellular pathology. Fluorescence in situ hybridization (FISH) is a well-known gene-based method to image genetic abnormalities. Development of a FISH microfluidic platform has offered the possibility of automation with significant time and cost reductions, which overcomes many drawbacks of the current protocols. Microfluidic devices are also powerful tools for single-cell analysis. Capturing the circulating tumor cells (CTCs) from blood samples is one of the most promising approaches to enable the early diagnosis of cancer. The microfluidic devices are also useful to isolate rare CTCs at high efficiency and purity. In this review, I outline recent FISH and CTC analyses using microfluidic devices, and describe their applications for the cellular diagnosis of cancers.

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Fish-on-a-chip: a sensitive detection microfluidic system for alzheimer's disease

Cited by 28 publications

References 147 publications

A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

CMOS Image Sensor-based Immunodetection by Refractive-Index Change

Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells

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