Fish scale derived hydroxyapatite incorporated 3D printed PLA scaffold for bone tissue engineering

Thomas, N. G.; Dalvi, Y. B.; Fijol, N; Shilpa, J.; Unni, Rekha; Binsi, P. K.; Varghese, M. G.; R, Reshmy.; Mathew, A. P.; Anil, Sukumaran

doi:10.1039/d3nj03005a

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…However, it is necessary to mention that the addition of the polycaprolactone (PCL) decreased the degradation rate. Furthermore, Thomas et al [ 45 ] found that an HA-incorporated 3D-printed PLA scaffold showcased compressive strength (13.4 ± 5.53 MPa) adequate for future in vivo evaluation. In this regard, synthetic PLA polymer Lactoprene ® 7415 was a material of choice in the presented study, due to its strictly determined mechanical properties and degradation rate.…”

Section: Resultsmentioning

confidence: 99%

Mechanical Characteristics of Individualized Biodegradable Augmentation Scaffold—In Vitro Pilot Study

Bjelica,

Prpić,

Drvar

et al. 2024

Materials

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The alveolar ridge reconstruction of vertical and combined bone defects is a non-predictable procedure with varying percentages of success. The greatest challenge for vertical and combined bone augmentation is to maintain mechanical stability of the bone graft; therefore, it is mandatory to provide and preserve space for bone regeneration. The development of biomaterials and 3D printing has enabled the use of polymer scaffolds in the reconstruction of alveolar ridge defects. The aim of this pilot study was to evaluate the mechanical characteristics of an innovative individualized biodegradable polylactic acid (PLA) scaffold, under dynamic conditions, simulating biodegradation and the influence of masticatory forces. After the design and 3D printing of PLA scaffolds, two groups of 27 scaffolds were formed according to the compression testing procedure. The compression tests were performed in occlusal and lateral directions. In each of the two groups, nine subgroups of three scaffolds were formed for different testing periods during in vitro degradation with a total period of 16 weeks. Results showed that biodegradation and load application had no significant influence on mechanical characteristics of tested scaffolds. It can be concluded that simulated masticatory forces and biodegradation do not significantly influence the mechanical characteristics of an individualized biodegradable augmentation scaffold.

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Section: Resultsmentioning

confidence: 99%

Mechanical Characteristics of Individualized Biodegradable Augmentation Scaffold—In Vitro Pilot Study

Bjelica,

Prpić,

Drvar

et al. 2024

Materials

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Fish scale–derived hydroxyapatite for alveolar ridge preservation

Prathibha,

Thomas,

Dalvi

et al. 2024

Biotech and App Biochem

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Alveolar ridge resorption following tooth extraction poses significant challenges for future dental restorations. This study investigated the efficacy of fish scale–derived hydroxyapatite (FSHA) as a socket preservation graft material to maintain alveolar bone volume and architecture. FSHA was extracted from *Labeo rohita* fish scales and characterized using Fourier transform infrared (FTIR) analysis. In vitro, biocompatibility and osteogenic potential were assessed using Saos‐2 human osteosarcoma cells. Cell viability, migration, and proliferation were evaluated using MTT and scratch assays. In vivo performance was assessed in a rat model, and FSHA was compared to a commercial xenograft (Osseograft) and ungrafted controls. Histological analysis was performed at 8‐week post‐implantation to quantify new bone formation. FTIR confirmed the purity and homogeneity of FSHA. In vitro, FSHA enhanced Saos‐2 viability, migration, and proliferation compared to controls. In vivo, FSHA demonstrated superior bone regeneration compared to Osseograft and ungrafted sites, with balanced graft resorption and new bone formation. Histological analysis revealed an active incorporation of FSHA into new bone, with minimal gaps and ongoing remodeling. Approximately 50%–60% of FSHA was resorbed by 8 weeks, closely matching the rate of new bone deposition. FSHA stimulated more bone formation in the apical socket region than in coronal areas. In conclusion, FSHA is a promising biomaterial for alveolar ridge preservation, exhibiting excellent biocompatibility, osteogenic potential, and balanced resorption. Its ability to promote robust bone regeneration highlights its potential as an effective alternative to currently used graft materials in socket preservation procedures.

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Taxifolin-loaded cellulose/l-arginine-chitosan hydrogel promoting bone defect repair through osteogenesis and angiogenesis

Yang,

Han,

Zhang

et al. 2024

International Journal of Biological Macromolecules

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Fish scale derived hydroxyapatite incorporated 3D printed PLA scaffold for bone tissue engineering

Abstract: Bone defect repair, particularly in the alveolar region, remains a significant hurdle in periodontics. In recent years, the spotlight in regenerative medicine has fallen on 3D-printed bone scaffolds, especially those...

Cited by 5 publications

References 47 publications

Mechanical Characteristics of Individualized Biodegradable Augmentation Scaffold—In Vitro Pilot Study

Mechanical Characteristics of Individualized Biodegradable Augmentation Scaffold—In Vitro Pilot Study

Fish scale–derived hydroxyapatite for alveolar ridge preservation

Taxifolin-loaded cellulose/l-arginine-chitosan hydrogel promoting bone defect repair through osteogenesis and angiogenesis

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