Fish vaccine: development, production and use of bacterial vaccines, with special reference to salmon

Aasjord, Per; Slinde, Erik

doi:10.1007/978-1-4615-5303-8_17

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…The aquaculture industry is recently facing a serious setback due to infectious diseases [15] leading to severe economic loss [2]. Although mortalities have been reported in all stages of life, maximum mortalities in all the cultivated fish species have been reported at larval stages.…”

Section: Discussionmentioning

confidence: 99%

Passive transfer of maternal antibodies and their existence in eggs, larvae and fry of Indian major carp, Labeo rohita (Ham.)

Swain

Dash

Bal

et al. 2006

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Passive transfer of maternal antibodies and their existence in eggs, larvae and fry of Indian major carp, Labeo rohita (Ham.)

Swain

Dash

Bal

et al. 2006

Fish & Shellfish Immunology

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“…Advancement of intensive aquaculture practices often have a negative impact on the pond environment leading to outbreaks of infectious diseases [1]. A wide range of antibiotics, drugs and chemicals are routinely used to control diseases.…”

mentioning

confidence: 99%

Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp, Labeo rohita (Ham.)

Nayak

Swain

Mukherjee

2007

Fish & Shellfish Immunology

199

101

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“…However, any intensive bio-production, whether on land or at sea, will experience disease problems [ 1 ]. Among the problems that the aquaculture industry has to address, infectious pathogens are the most important [ 2 , 3 , 4 ]. In 1993, the economic losses caused by the infectious pathogens are more than ten percent of the total value of the fishery industry [ 5 ], and the loss ratio is increased gradually in the last decades.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Multi-Wall Carbon Nanotubes Enhance Transfection and Expression Efficiency of Plasmid DNA in Fish Cells

Liu

Wang

et al. 2016

IJMS

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DNA vaccines are considered to be the most promising method against infectious diseases in the aquaculture industry. In the present study, we investigated the potency of ammonium group-functionalized multi-walled carbon nanotubes (MWCNTs) in enhancing the transfection and expression efficiency of plasmid DNA (pEGFP-vp5) in Ctenopharyngodon idellus kidney (CIK) cells. Agarose gel shift assay results show that ammonium group-functionalized carbon nanotubes are able to condense DNA in varying degrees. Scanning electron microscope (SEM) images shows that CIK cells show a great affinity for MWCNTs-NH3+ and the CNTs covering the cell surface tend to orient their tips perpendicularly to the cell surface, and appear to be “needle-pricking the cells”. Transmission electron microscope (TEM) images confirmed that MWCNTs-NH3+ penetrate the cell membranes and are widely dispersed in the CIK cell. Real-time PCR was used to detect the transfection efficiency through the expression of the outer capsid protein (VP5). The results showed that the MWCNTs-NH3+:DNA complexes are able to transfect CIK cells effectively at different charge ratio than naked DNA. Subsequent studies confirmed that both functional groups and charge ratio are important factors that determine the transfection efficiency of plasmid DNA. All these results indicated that MWCNTs-NH3+:DNA complexes could be suitable for developing DNA vaccine for the control of virus infection in the aquaculture industry.

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Fish vaccine: development, production and use of bacterial vaccines, with special reference to salmon

Cited by 6 publications

References 36 publications

Passive transfer of maternal antibodies and their existence in eggs, larvae and fry of Indian major carp, Labeo rohita (Ham.)

Passive transfer of maternal antibodies and their existence in eggs, larvae and fry of Indian major carp, Labeo rohita (Ham.)

Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp, Labeo rohita (Ham.)

Functionalized Multi-Wall Carbon Nanotubes Enhance Transfection and Expression Efficiency of Plasmid DNA in Fish Cells

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