Fish venom (Pterios volitans) peptide reduces tumor burden and ameliorates oxidative stress in Ehrlich’s ascites carcinoma xenografted mice

Balasubashini, M. Sri; Karthigayan, S.; Somasundaram, S. T.; Balasubramanian, T.; Viswanathan, Vivek; Raveendran, P; Menon, Venugopal P.

doi:10.1016/j.bmcl.2006.09.025

Cited by 14 publications

(5 citation statements)

References 26 publications

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“…In our laboratory, previous findings have suggested that lionfish venom has antimetastatic effects on EAC cells in vivo and reduced the oxidative stress produced in the liver by the peritoneal carcinoma (Sri Balasubashini et al 2006). Hence, the present study well corroborates with previous findings and suggests the presence of antitumor principles in octopus venom.…”

Section: Discussionsupporting

confidence: 95%

PGE fromOctopus aeginaInduces Apoptosis in Ehrlich's Ascites Carcinoma of Mice

Karthigayan

Balasubashini

Balasubramanian

et al. 2007

Toxicology Mechanisms and Methods

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The present study was carried out to assess the antitumor effect of venomous peptide from the cephalopod Octopus aegina on Ehrlich's ascites carcinoma (EAC). Male albino Swiss mice were used in the present study. Four groups of animals were treated with three doses of the sublethal dose of venom, 15, 75, and 150 mug/kg body weight (intraperitoneal injection), along with the standard drug 5-fluorouracil (20 mg/kg b.w.). After 10 days of treatment, six animals from each group were sacrificed for the biochemical analysis and the rest were left to calculate the mean survival time. In EAC-bearing mice, mean lifespan, tumor volume, hemoglobin, red blood cells, and lymphocytes were significantly decreased when compared to the normal animals. While body weight, neutrophils, and viable tumor cell count were increased in the EAC-bearing mice, these changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as DNA, RNA, and protein, were altered in the EAC-bearing mice and observed to be near normal in the treatment groups. The caspase-3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC-bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, octopus venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Therefore, the venom from O. aegina has a potential antitumor effect on the EAC-bearing mice.

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Section: Discussionsupporting

confidence: 95%

PGE fromOctopus aeginaInduces Apoptosis in Ehrlich's Ascites Carcinoma of Mice

Karthigayan

Balasubashini

Balasubramanian

et al. 2007

Toxicology Mechanisms and Methods

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“…Also a significant elevation in MAD level was recorded in the group of animals bearing tumors ( Table 2). Similar observations in other laboratories were reported [28,29]. MDA, the end product of lipid peroxidation (LPO) was reported to be higher in carcinomatous tissue than in non diseased organs.…”

Section: Discussionsupporting

confidence: 88%

Evaluation of Anti-Oxidant Status and Radioprotective Activity of a Novel Anti-Cancer Drug in Mice

Yousri¹,

Noaman²,

Shawi³

et al. 2011

JCT

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Various approaches have been developed for diminishing the effects of radiation on normal tissues or enhancing tumor cell killing by ionizing radiation. Recently, there has been an increase in the interest in research on synthetic and/or natural radioprotective agents. An important potential use for these agents is to modify and improve the outcome of radiation therapy. The aim of this study was to examine the potential radioprotective role and antioxidant potency of the novel synthetic anticancer agent, quinoline sulfonamide (PIQSA) against tissue injury and oxidative stress induced by the exposure to gamma radiation and/or incidence of cancer in experimental animals. Mice (normal and bearing solid tumors) administered PIQSA (0.350 mg/kg body weight ip. three times a week for 21 days. At the last week of 30 days experimental period, an animal group was subjected to three successive doses of γ-radiation each of 2 Gy; another group was treated with combined administration of PIQSA 20 minutes before γ-irradiation. Some biochemical parameters (LPx, GSH, SOD, and CAT in liver homogenates, also plasma lipid profile (total lipids, total cholesterol, total triglycerides (TG), HDLc and LDLc were measured. To examine any adverse effect which could be attained by chemical treatment, liver enzymes (AST, ALT), and kidney function (creatinine and urea) were estimated in blood plasma, in addition to examination of some haematological indexes. The results indicated that the deleterious effects due to exposure to γ-radiation, and/or incidence of cancer on most of the estimated parameters could be controlled to a certain extent by administration of PIQSA to animals prior to irradiation. The results also confirmed that there were no significant adverse effects on mice due to the treatment with this chemical compound

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“…A 7.6 kDa peptide from P. volitans venom was isolated and tested for its effectiveness against cancer cells [ 163 ]. Although the structure of the peptide was not elucidated, it was found that it selectively induced apoptosis in HEp2 and HeLa cells in vitro while having no deleterious effects on human lymphocytes.…”

Section: Venom Componentsmentioning

confidence: 99%

Bioactive Components in Fish Venoms

Ziegman

Alewood

2015

Toxins

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Animal venoms are widely recognized excellent resources for the discovery of novel drug leads and physiological tools. Most are comprised of a large number of components, of which the enzymes, small peptides, and proteins are studied for their important bioactivities. However, in spite of there being over 2000 venomous fish species, piscine venoms have been relatively underrepresented in the literature thus far. Most studies have explored whole or partially fractioned venom, revealing broad pharmacology, which includes cardiovascular, neuromuscular, cytotoxic, inflammatory, and nociceptive activities. Several large proteinaceous toxins, such as stonustoxin, verrucotoxin, and Sp-CTx, have been isolated from scorpaenoid fish. These form pores in cell membranes, resulting in cell death and creating a cascade of reactions that result in many, but not all, of the physiological symptoms observed from envenomation. Additionally, Natterins, a novel family of toxins possessing kininogenase activity have been found in toadfish venom. A variety of smaller protein toxins, as well as a small number of peptides, enzymes, and non-proteinaceous molecules have also been isolated from a range of fish venoms, but most remain poorly characterized. Many other bioactive fish venom components remain to be discovered and investigated. These represent an untapped treasure of potentially useful molecules.

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Fish venom (Pterios volitans) peptide reduces tumor burden and ameliorates oxidative stress in Ehrlich’s ascites carcinoma xenografted mice

Cited by 14 publications

References 26 publications

PGE fromOctopus aeginaInduces Apoptosis in Ehrlich's Ascites Carcinoma of Mice

PGE fromOctopus aeginaInduces Apoptosis in Ehrlich's Ascites Carcinoma of Mice

Evaluation of Anti-Oxidant Status and Radioprotective Activity of a Novel Anti-Cancer Drug in Mice

Bioactive Components in Fish Venoms

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