Fishing for exosomes

Alderton, Gemma

doi:10.1038/nrc3990

Cited by 39 publications

(27 citation statements)

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“…9–11 Probing of tumor-derived circulating exosomes has been emerging to better serve non-invasive cancer diagnosis and monitoring of treatment response. 12 However, exosome biogenesis at the molecular level is still not well understood, and clinical utilization of exosomes lags, due to current technical challenges in rapid isolation and molecular identification of exosomes. 8, 13 …”

Section: Introductionmentioning

confidence: 99%

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

et al. 2016

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Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in-situ, multiplexed detection using immunomagnetic beads. The ExosSearch chip offers robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.

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Section: Introductionmentioning

confidence: 99%

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

et al. 2016

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“…MiRNAs also have novel potential for disease diagnostics. Specific detection of cancer cell‐derived exosomes and their miRNAs in the blood shows promise for improved early cancer diagnostics [Melo et al, ; Alderton, ; Munson and Shukla, ]. In particular, exosomal circular RNA in plasma may offer better early cancer diagnosis [Li et al, ; Meng et al, ].…”

Section: Micrornas: Potential Drug Targets?mentioning

confidence: 99%

MicroRNAs as CNS Drug Targets

Genevieve

2016

Drug Development Research

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“…Exosome, a major component of EVs, carries the fundamental biological elements such as protein, mRNA, long non-coding RNA (lncRNA), and, especially small RNA (sRNA) [1]. Recently, exosome has become an emerging source of biomarkers of various human diseases like neurodegenerative diseases and cardiovascular diseases[2]. Using peripheral blood to detect exosomal inclusions could satisfy the rapid and noninvasive requirement of chronic disease prediction[3].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Commercially Available Exosomal Isolation Kits from Human Plasma

Yuzhe

Zhen

Guo

et al. 2019

Preprint

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Exosomes are cell-derived lipid bilayer particles which are abundant in biological fluids.14 Exosome is an emerging source of biomarkers to diagnose various human diseases. Sequencing 15 based exosomal studies could provide a comprehensive view of exosomal RNA and protein. To16extracted these inclusions, exosomes should be isolated from the plasma first. Several exosome 17 isolation methods were introduced since the discover of exosome. To promote the clinical 18 application of exosomal inclusions, different isolation methods should be compared. We isolated 19 exosomes from human plasma by using user-friendly and commercially available kits, SBI 20 ExoQuick and QIAGEN exoRNeasy. Subsequently, small RNA sequencing was performed with two 21 groups of isolated exosome samples and one group of plasma samples. No fundamental differences 22 of exRNA yield between SC and EQ were found. In RNA profile analysis, the small RNA aligned 23 reads, miRNA pattern, sample clustering varied as a result of methodological differences. Small 24RNA isolated by ExoQuick presented better data quality and RNA profile than exoRNeasy. This 25 study compared sRNA sequencing data generated from two exosome isolation kits, it provides a 26 reference for future small RNA studies and biomarker prediction in human plasma exosome. 27 Keywords: 28 29 1. Introduction 30 Extracellular vesicles (EVs) are small lipid bilayer particles that are commonly observed in 31 human body fluids involving in cell-to-cell communication. Exosome, a major component of EVs, 32 carries the fundamental biological elements such as protein, mRNA, long non-coding RNA (lncRNA), 33 and, especially small RNA (sRNA) [1]. Recently, exosome has become an emerging source of 34 biomarkers of various human diseases like neurodegenerative diseases and cardiovascular 35 diseases[2]. Using peripheral blood to detect exosomal inclusions could satisfy the rapid and 36 noninvasive requirement of chronic disease prediction[3]. Particularly, the exosomal sRNA including 37 microRNA (miRNA) are of great diagnostic interest since they are important post-translational 38 regulators and protected by lipid bilayer structure from exposure to RNases [4]. Thus, method of 39 extracting RNA from exosomes and RNA-seq library preparation kits are developed [5-7]. Due to the 40 nature of sRNA diversity in exosomes, exosomal sRNA patterns are easily interfered, it is extremely 41 hard to reproduce the same results which have been obtained from the previous experiments [4]. A 42 few works have compared different methods of exosomal sRNA extraction by measuring isolated 43 exosome integrity and isolation efficiency from different tissues. However, previous works didn't 44 systematically analyze transcriptomic or proteomic data[8]. Our study focused on comparing next-65 QIAGEN exoRNeasy Serum/Plasma Kit, were chosen as they represent two isolation method, the66 polymer-based precipitation and the column-based chromatography, respectively. This study is 67 focusing on the impact of different exosome isola...

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Fishing for exosomes

Cited by 39 publications

References 1 publication

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

MicroRNAs as CNS Drug Targets

Evaluation of Commercially Available Exosomal Isolation Kits from Human Plasma

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