Five-assay microbiological system for the screening of antibiotic residues

A microbial inhibition assay in microplates using Bacillus licheniformis for detection of enrofloxacin and sulfamethazine in chicken spiked kidney, liver and muscle tissues

“…According to our results, detection limits were higher for ENR in Tumini et al (2017Tumini et al ( , 2019, it was revealed that obtained detection limits for ENR in chicken kidney and muscle was slightly higher (124.03 and 136.38 µg/kg, respectively) but the ENR in liver was at a similar level (90.02 µg/kg). Moreover, the detection limits for sulfonamide in our study were lower than those determined by Tumini et al (2019) which could be related to differences in matrix types and composition of bioassays.…”

“…According to our results, detection limits were higher for ENR in Tumini et al (2017Tumini et al ( , 2019, it was revealed that obtained detection limits for ENR in chicken kidney and muscle was slightly higher (124.03 and 136.38 µg/kg, respectively) but the ENR in liver was at a similar level (90.02 µg/kg). Moreover, the detection limits for sulfonamide in our study were lower than those determined by Tumini et al (2019) which could be related to differences in matrix types and composition of bioassays. Considering the above results, findings of this study indicated that MIA in microplates using B. licheniformis for ENR and SMT detection in chicken tissues could detect residues at the levels below or slightly above the respective MRLs compared to studies using G. stearothermophilus which mostly found higher detection limits for these antimicrobials.…”

“…Microbial inhibition assays (MIA) can be divided into two test tube and petri dish methods. Test tube‐based assays provide a colorimetric response with visual interpretation or absorbance measurement in a short incubation time (3–4 h) and are more applicable for screening of antimicrobial residues in large number of samples compared with petri dish methods relying on determination of inhibition zone after 16–18 h of incubation (Tumini et al., 2019).…”

“…Hence, in some studies methods made up of other microorganisms such as Bacillus megaterium , Bacillus cereus , Bacillus subtilis and Bacillus licheniformis that are sensitive to different groups of antimicrobials have also been proposed. It has been reported that B. licheniformis ATCC 14,580 has acceptable sensitivity to both quinolones and sulfonamides (Nagel et al., 2013; Tumini et al., 2019).…”

Development of a new dual electrochemical immunosensor for a rapid and sensitive detection of enrofloxacin in meat samples

“…These methods include enzyme-linked immunosorbent assays (Hu et al, 2019;Z. Wang et al, 2014;Zhang et al, 2011), immunochromatographic strip tests (Chen et al,4 2012; Huang et al, 2013), microbiological inhibition tests (Mata et al, 2014;Tumini et al, 2019;Q. Wu et al, 2019) and fluorescent (Dolati et al, 2018;Ha et al, 2016) and colorimetric sensors (Rezende et al, 2019).…”