Five New Cytotoxic Metabolites from the Marine Fungus Neosartorya pseudofischeri

Abstract

The marine fungus Neosartorya pseudofischeri was isolated from Acanthaster planci from the South China Sea. In a preliminary bioactivity screening, the crude methanol extract of the fungal mycelia showed significant inhibitory activity against the Sf9 cell line from the fall armyworm Spodoptera frugiperda. Five novel compounds, including 5-olefin phenylpyropene A (1), 13-dehydroxylpyripyropene A (4), deacetylsesquiterpene (7), 5-formyl-6-hydroxy-8-isopropyl-2- naphthoic acid (9) and 6,8-dihydroxy-3-((1E,3E)-penta-1,3-dien-1-yl)isochroman-1-one (10), together with eleven known compounds, phenylpyropene A (2) and C (3), pyripyropene A (5), 7-deacetylpyripyropene A (6), (1S,2R,4aR,5R,8R,8aR)-1,8a-dihydroxy-2-acetoxy-3,8-dimethyl-5- (prop-1-en-2-yl)-1,2,4a, 5,6,7,8,8a-octahydronaphthalene (8), isochaetominine C (11), trichodermamide A (12), indolyl-3-acetic acid methyl ester (13), 1-acetyl-β-carboline (14), 1,2,3,4-tetrahydro-6-hydroxyl-2-methyl-l,3,4-trioxopyrazino[l,2-a]-indole (15) and fumiquinazoline F (16), were obtained. The structures of these compounds were determined mainly by MS and NMR data. The absolute configuration of 9 was assigned by the single-crystal X-ray diffraction studies. Compounds 1–11 and 15 showed significant cytotoxicity against the Sf9 cells from S. frugiperda.

Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives

“…This compound was previously isolated from several types of organisms. Thereunder plants like Ailanthus malabarica and Hypodematiumsquamuloso-pilosum ( Joshi et al, 1977 ; Zhou et al, 1998 ), a fungus Neosartorya pseudofischeri ( Lan et al, 2016 ), a sponge Tedaniaignis ( Dillman and Cardellina, 1991 ), as well as several bacteria, e.g., Steptomyces and Pseudomonas spp. ( Proksa et al, 1990 ; Shin et al, 2010 ; Huang et al, 2011 ; Chen et al, 2013 ; Lee et al, 2013 ; Broberg et al, 2017 ).…”

Labradorins with Antibacterial Activity Produced by Pseudomonas sp.

“…The 1-Acetyl-3-carboxy-β-carboline has previously only been found in plants [ 15 , 17 ], and this is the first report on the production of this compound by a bacterium. Conversely, 1-acetyl-β-carboline has previously been isolated from several types of organisms, including plants [ 14 , 18 ], a fungus [ 19 ], a sponge [ 20 ], and several bacteria e.g., [ 21 ].…”

A new species of Trichoderma hypoxylon harbours abundant secondary metabolites

“…Trichodermamide and aspergillazines are two kind of modified dipeptides1525. Trichodermamide A has been found from a marine-derived T. virens 24, it is also be obtained from marine-derived fungi Spicaria elegans 25 and Neosartorya pseudofischeri 32 and endophytic fungus Trichoderma spirale 33. The yield of trichodermamide A produced by these fungi were 1.58 mg/L24, 0.73 mg/L25, 0.12 mg/L32 and 8.98 mg/L33 individually.…”