Fixation for the 1990's: a Review of Needs and Accomplishments

Dapson, Richard W.

doi:10.3109/10520299309104670

Cited by 71 publications

(42 citation statements)

References 15 publications

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“…178 The basic mechanism of fixation with formaldehyde is the formation of addition products (adducts) between the formalin and uncharged reactive amino groups (-NH or NH 2 ) that eventually will form cross-links. 75 The formation of methylol adducts inactivates nucleases and proteases. 261 Once the addition product (reactive hydroxymethyl compound) is formed, additional cross-links develop.…”

Section: Fixationmentioning

confidence: 99%

When Tissue Antigens and Antibodies Get Along

2013

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Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC) today is used in the investigation of a broad range of disease processes with applications in diagnosis, prognostication, therapeutic decisions to tailor treatment to an individual patient, and investigations into the pathogenesis of disease. This review addresses the technical aspects of immunohistochemistry (and, to a lesser extent, immunocytochemistry) with attention to the antigen-antibody reaction, optimal fixation techniques, tissue processing considerations, antigen retrieval methods, detection systems, selection and use of an autostainer, standardization and validation of IHC tests, preparation of proper tissue and reagent controls, tissue microarrays and other high-throughput systems, quality assurance/quality control measures, interpretation of the IHC reaction, and reporting of results. It is now more important than ever, with these sophisticated applications, to standardize the entire IHC process from tissue collection through interpretation and reporting to minimize variability among laboratories and to facilitate quantification and interlaboratory comparison of IHC results.

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Section: Fixationmentioning

confidence: 99%

When Tissue Antigens and Antibodies Get Along

2013

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“…With the recognition that formalin fixation causes major chemical changes in antigens by unknown mechanisms, several attempts were made to modify the methods of fixation, and a variety of modified fixatives have been proposed for preservation of certain antigens Dapson 1993;Elias 1990;Colvin et al 1988;DeLellis 1988;Larsson 1988;Hopwood 1985). However, no ideal fixative has been found that can be used universally in IHC.…”

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confidence: 99%

Antigen Retrieval Immunohistochemistry: Past, Present, and Future

Shi

Côté²,

Taylor

1997

J Histochem Cytochem.

480

331

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SUMMARYThe antigen retrieval (AR) technique, which is predominantly based on hightemperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a "test battery" and the concept of "maximal retrieval" applied to the selection of optimal test protocols for the standardization of AR. (J Histochem Cytochem 45:327-343, 1997)

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“…Acredita-se que o sistema avidina-biotina utilizado no presente trabalho tenha maior sensibilidade do que o sistema peroxidase-anti-peroxidase (Bullock e Petrusz, 1989). Além disso, o uso de outro fixador que não o formaldeído é de difícil implementação na rotina de laboratório de diagnóstico histológico (Dapson, 1993).…”

Section: Methodsunclassified

Otimização da imunoistoquímica para detecção de herpesvírus bovino tipo 5 (BHV-5) em tecidos do sistema nervoso central fixados com formaldeído

Hübner

Pescador

Corbellini

et al. 2005

Arq. Bras. Med. Vet. Zootec.

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RESUMOCom o objetivo de otimizar a técnica de imunoistoquímica para detecção de herpesvírus bovino tipo 5 (BHV-5) em tecidos do sistema nervoso central fixado em formaldeído, foram avaliados diferentes métodos de digestão enzimática, diferentes anticorpos e reagentes para bloqueio de reações inespecíficas. As reações apresentaram a máxima intensidade de coloração específica e a quantidade mínima de coloração de fundo quando foram usadas protease de Streptomyces griseus (0,1%) ou proteinase K de Tritirachium album limber (0,05%), mediante incubação durante 15 minutos a 37°C. Entre os anticorpos monoclonais analisados, dois foram capazes de detectar BHV-5. As reações inespecíficas foram bloqueadas mais efetivamente pela incubação do tecido com caseína (0,5%), durante cinco minutos, ou leite em pó (2,5%), durante 60 minutos, ou soro eqüino (2,5%), durante 60 minutos. Com a técnica otimizada foi possível a detecção de BHV-5 em material de arquivo.Palavras-chave: imunoistoquímica, recuperação antigênica, BHV-5, sistema nervoso central ABSTRACT In order to optimize immunohistochemical technique (IHC) for detection of Bovine herpesvirus type 5 (BHV-5) on formalin-fixed sections of central nervous system, different methods of enzymatic digestion, use of different antibodies and products for blocking of nonspecific reactivity were evaluated. The reactions showed the highest intensity of specific coloration and the minimum amounts of background when protease from

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Fixation for the 1990's: a Review of Needs and Accomplishments

Cited by 71 publications

References 15 publications

When Tissue Antigens and Antibodies Get Along

When Tissue Antigens and Antibodies Get Along

Antigen Retrieval Immunohistochemistry: Past, Present, and Future

Otimização da imunoistoquímica para detecção de herpesvírus bovino tipo 5 (BHV-5) em tecidos do sistema nervoso central fixados com formaldeído

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