Fixed bed porous glass sphere (porosphere) bioreactors for animal cells

Looby, D.; Griffiths, J. B.

doi:10.1007/bf00365079

Cited by 68 publications

(16 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In principle, cells attach to the carriers when solid glass spheres [77], porous carriers (e.g. Siran (glass) spheres) [78], or stainless steel gauze and coupons [79] are used, or attach and are entrapped when porous sponge-like materials, such as FibraCell [80], are used. In both of the latter cases, also cells that do not readily attach (such as hybridoma cells) can be cultivated in packed bed reactors.…”

Section: Fixed Bed Reactor/packed Bed Reactormentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

View full text Add to dashboard Cite

Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for treatment of acquired or inherited diseases. In this review, the present state-of-the-art of the production of retroviral vectors and the most important parameters, such as the choice of the producer cell line, stability issues, medium additives, serum, type of bioreactor, that influence production issues is presented and discussed in light of an optimal vector production. The available literature data clearly indicate that, on one hand, the choice of the producer cell line is of utmost importance for obtaining a high level producer cell line, and that, on the other hand, the optimization of the medium, e.g. the replacement of glucose by fructose, has a potential for improving vector production rates and titers. Finally, the use of high-density perfusion culture systems for adherent as well as for suspension cells presents the best choice for a production system, because high cell densities imply high reactor specific production rates, which must be associated with a rapid harvest of the produced vector, thus avoiding vector inactivation due to an extended residence time. The overall optimization of the cultivation and production parameters will have a significant impact on the use of retroviral vectors for gene therapy purposes.

show abstract

Section: Fixed Bed Reactor/packed Bed Reactormentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

View full text Add to dashboard Cite

show abstract

“…However the ultimate in maximising surface area is a porous bead with fully interlinking passageways. This method was pioneered by Verax (Hayman et al, 1987;Runstadler and Cemek, 1988), followed by the Percell (Nilsson, 1988) and Siran systems (Looby & Griffiths, 1988. With the correct pore diameter (approximately 2-3 times greater than the cell diameter) (Messing, 1979) good cell and nutrient penetration is possible.…”

Section: Cell Carriers (Microcarriers)mentioning

confidence: 99%

Animal cells ? the breakthrough to a dominant technology

Griffiths

1990

Cytotechnology

View full text Add to dashboard Cite

“…In Festbettreaktoren treten nichtnewtonsche Fluide unter anderem bei biochemischen Prozessen, wie der Herstellung von Antikörpern und tierischen Zellkulturen, bei der Alkoholproduktion und Fermentationsprozessen sowie bei der stereoselektiven Biokonversion auf [3][4][5][6][7]. Weitere Festbettprozesse aus der Abwasserbehandlung und der biologischen Gasreinigung, bei denen nichtnewtonsche Fluide involviert sind, sind beispielsweise die Filtration von Klärschläm-men mit Sandfiltern sowie die Abtrennung von Schwefelkohlenstoffen, flüchtigen organischen Verbindungen und chlorierten aliphatischen Kohlenwasserstoffen [8][9][10][11].…”

Section: Introductionunclassified