Flagellar radial spoke: A model molecular genetic system for studying organelle assembly

Curry, Alice M.; Rosenbaum, Joel L.

doi:10.1002/cm.970240403

Cited by 71 publications

(61 citation statements)

References 38 publications

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“…We used RNAi to target radial spokes and the central pair apparatus of T. brucei. To target radial spokes, we chose RSP3, which is required for attachment of the spoke to the axoneme in C. reinhardtii (12). The major components of the central pair apparatus are two singlet microtubules, C1 and C2, together with their specific projections and intermicrotubule bridges (62).…”

Section: Resultsmentioning

confidence: 99%

Flagellar Motility Contributes to Cytokinesis inTrypanosoma bruceiand Is Modulated by an Evolutionarily Conserved Dynein Regulatory System

et al. 2006

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The flagellum of Trypanosoma brucei is a multifunctional organelle with critical roles in motility and other aspects of the trypanosome life cycle. Trypanin is a flagellar protein required for directional cell motility, but its molecular function is unknown. Recently, a trypanin homologue in Chlamydomonas reinhardtii was reported to be part of a dynein regulatory complex (DRC) that transmits regulatory signals from central pair microtubules and radial spokes to axonemal dynein. DRC genes were identified as extragenic suppressors of central pair and/or radial spoke mutations. We used RNA interference to ablate expression of radial spoke (RSP3) and central pair (PF16) components individually or in combination with trypanin. Both rsp3 and pf16 single knockdown mutants are immotile, with severely defective flagellar beat. In the case of rsp3, this loss of motility is correlated with the loss of radial spokes, while in the case of pf16 the loss of motility correlates with an aberrant orientation of the central pair microtubules within the axoneme. Genetic interaction between trypanin and PF16 is demonstrated by the finding that loss of trypanin suppresses the pf16 beat defect, indicating that the DRC represents an evolutionarily conserved strategy for dynein regulation. Surprisingly, we discovered that four independent mutants with an impaired flagellar beat all fail in the final stage of cytokinesis, indicating that flagellar motility is necessary for normal cell division in T. brucei. These findings present the first evidence that flagellar beating is important for cell division and open the opportunity to exploit enzymatic activities that drive flagellar beat as drug targets for the treatment of African sleeping sickness.

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Section: Resultsmentioning

confidence: 99%

Flagellar Motility Contributes to Cytokinesis inTrypanosoma bruceiand Is Modulated by an Evolutionarily Conserved Dynein Regulatory System

et al. 2006

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“…The radial spokes consist of a thin stalk attached to the A-tubule of the doublet microtubules and a bulbous head projecting toward the central apparatus [reviewed in Curry and Rosenbaum, 1993]. They repeat in pairs (Chlamydomonas) or triplet groups (Tetrahymena) every 96 nm and maintain a right-handed helix along the length of the axoneme [Warner, 1970;Warner and Satir, 1974;Goodenough and Heuser, 1985].…”

Section: Structural Analysis Of Central Pair and Radial Spoke Functionmentioning

confidence: 99%

The radial spokes and central apparatus: Mechano‐chemical transducers that regulate flagellar motility

Smith

Yang

2003

Cell Motil. Cytoskeleton

269

259

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“…Radial spokes in Chlamydomonas flagella have been shown to be composed of 17 proteins, of which five are localized at the spoke head and 12 in the spoke stalk (Huang et al, 1982). Several radial spoke proteins have been cloned and characterized (Curry et al, 1992;Curry and Rosenbaum, 1993). Radial spoke protein 3 (RSP3), for example, present at the proximal end of the spoke stalk, helps in anchoring the radial spoke to the outer doublet Diener et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the AscidianCiona intestinalis

Padma

Satouh

Wakabayashi

et al. 2003

MBoC

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Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidian Ciona intestinalis by using an antiserum against whole axonemes. We report here a 37-kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22ϩ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported in Chlamydomonas and sea urchin flagella, and thus represents a novel axonemal LRR protein. Immunoelectron microscopy by using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact seems to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.

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Flagellar radial spoke: A model molecular genetic system for studying organelle assembly

Cited by 71 publications

References 38 publications

Flagellar Motility Contributes to Cytokinesis inTrypanosoma bruceiand Is Modulated by an Evolutionarily Conserved Dynein Regulatory System

Flagellar Motility Contributes to Cytokinesis inTrypanosoma bruceiand Is Modulated by an Evolutionarily Conserved Dynein Regulatory System

The radial spokes and central apparatus: Mechano‐chemical transducers that regulate flagellar motility

Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the AscidianCiona intestinalis

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