Flagellin-deficient outer membrane vesicles as adjuvant induce cross-protection of Salmonella Typhimurium outer membrane proteins against infection by heterologous Salmonella serotypes

Liu, Qiong; Tan, K.; Yuan, Jun; Song, Kuangyu; Li, Rong; Huang, Xiaotian; Liu, Qing

doi:10.1016/j.ijmm.2018.06.001

Cited by 30 publications

(21 citation statements)

References 42 publications

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“…A more acceptable statement is that the immune responses elicited by bacterial OMVs involve the mixtures of cellular and humoral immunity [22,23]. In addition, OMV immunization of different origins even from allogeneic species has discrepancy in types of host immunity, which may be attributed to different components carried by OMVs [24]. Different from other bacteria-derived OMVs, our present findings indicated that immunization with OMVs Figure 6: Mechanisms of A. baumannii-derived OMVs act as defenders against bacterial infection.…”

Section: Discussionmentioning

confidence: 72%

Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response

Cai

Kesavan

Cheng

et al. 2019

Journal of Immunology Research

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Acinetobacter baumannii, as a nonfermentation Gram-negative bacterium, mainly cause nosocomial infections in critically ill patients. With the widespread of multidrug-resistant Acinetobacter baumannii, the urgency of developing effective therapy options has been emphasized nowadays. Outer membrane vesicles derived from bacteria show potential vaccine effects against bacterial infection in recent study. Our present research is aimed at investigating the mechanisms involved in immune protection of mice after outer membrane vesicle immunization. As our data showed, the outer membrane vesicle from an Acinetobacter baumannii clinical strain could activate bone marrow-derived dendritic cells (BMDCs) to promote Th2 activity together with humoral immune responses to Acinetobacter baumannii-induced sepsis, which might enlighten people to have a better understanding of OMVs' role as a vaccine to prevent bacterial infections.

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Section: Discussionmentioning

confidence: 72%

Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response

Cai

Kesavan

Cheng

et al. 2019

Journal of Immunology Research

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“…Globally, Salmonella is the predominant cause of typhoidal and non-typhoidal gastroenteritis infections leading to significant mortality and morbidity (Liu et al 2018). Non-typhoidal Salmonella alone causes~93 million cases of gastroenteritis worldwide annually (Eng et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium

Reddy

Makam

Kota

et al. 2020

Appl Microbiol Biotechnol

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In the present study, we have generated a murine monoclonal antibody (mAb) named Sal-06 by using the crude outer membrane protein preparation of Salmonella enteric subsp. enterica serovar Typhimurium ATCC 14028 strain as antigen. Sal-06mAb belonging to IgG1 isotype demonstrated broad cross-reactivity to standard and isolated strains of genus Salmonella and others such as Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis. Cross-reactivity across several bacterial genera indicated that the epitopes reactive to Sal-06mAb are conserved among these members. Neutralizing effects of Sal-06mAb on Salmonella growth and survival was evaluated in vitro using bacteriostatic and bactericidal activity with and without complement and bacterial invasion inhibition assay. Sal-06mAb demonstrated a bacteriostatic effect on the growth of S. typhimurium ATCC 14028 strain which is both time and concentration (of mAb) dependent. It was also found that the bacterial growth inhibition was complement independent. When the bacterial cells were preincubated with Sal-06mAb, it reduced the adherence and invasion of bacterial cells into A549 epithelial cell line. This was confirmed by CFU count analysis, phase contrast, and fluorescence microscopy. Scanning electron microscope (SEM) imaging confirmed the antimicrobial effects of Sal-06mAb on S. typhimurium ATCC 14028. The development of broadly reactive and cross protective Sal-06mAb opens new possibilities for immunotherapy of sepsis caused by Gram-negative Enterobacteriaceae members.

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“…160 OMVs have been used in vaccine development for the prevention of bacterial infections, such as those caused by Neisseria meningitidis (N. meningitidis) serogroup B 161 or Shigella flexneri, 162 and NTS-derived OMVs have recently been tested in animal models, yielding promising immunogenicity and protection data. [163][164][165] One of the main problems of both Salmonella OMVs and LAVs is the toxicity of LPS. Several strategies, including dephosphorylation of lipid A 166 or mutation of the wzy gene encoding the OAg polymerase involved in LPS synthesis 167 can be applied in order to detoxify LPS without compromising immunogenicity.…”

Section: Omv-based Vaccinesmentioning

confidence: 99%

“…163 Immunization with OMVs derived from flagellin-deficient S. Typhimurium has proved able to provide protection against homologous and heterologous serovars (S. Enteriditis and S. Choleraesuis), suggesting that it might also be a suitable means of achieving cross-reactive immunity. 163,164…”

Section: Omv-based Vaccinesmentioning

confidence: 99%

Pathogenic signature of invasive non-typhoidal Salmonella in Africa: implications for vaccine development

Piccini

Montomoli

2020

Human Vaccines & Immunotherapeutics

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Invasive non-typhoidal Salmonella (iNTS) infections are a leading cause of bacteremia in Sub-Saharan Africa (sSA), thereby representing a major public health threat. Salmonella Typhimurium clade ST313 and Salmonella Enteriditis lineages associated with Western and Central/Eastern Africa are among the iNTS serovars which are of the greatest concern due to their case-fatality rate, especially in children and in the immunocompromised population. Identification of pathogen-associated features and host susceptibility factors that increase the risk for invasive non-typhoidal salmonellosis would be instrumental for the design of targeted prevention strategies, which are urgently needed given the increasing spread of multidrug-resistant iNTS in Africa. This review summarizes current knowledge of bacterial traits and host immune responses associated with iNTS infections in sSA, then discusses how this knowledge can guide vaccine development while providing a summary of vaccine candidates in preclinical and early clinical development.

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Flagellin-deficient outer membrane vesicles as adjuvant induce cross-protection of Salmonella Typhimurium outer membrane proteins against infection by heterologous Salmonella serotypes

Cited by 30 publications

References 42 publications

Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response

Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response

Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium

Pathogenic signature of invasive non-typhoidal Salmonella in Africa: implications for vaccine development

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