Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore

Abstract

<p>Flavin-tag: we developed a facile method to label a target protein with a flavin. By using a short peptide (Flavin-tag), recognized by a flavinylating enzyme, FMN can be covalently incorporated. Attachment of the flavin chromo- and fluorophore can be performed <i>in vivo</i> and <i>in vitro</i> rendering the Flavin-tag a flexible tool for protein labeling.</p>