Flavobacterium chungbukense sp. nov., isolated from soil

Abstract

A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100 T , was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100 T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100 T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953 T (97.6 %) and Flavobacterium johnsoniae KACC 11410 T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5-97.0 %. Growth occurred at 4-30 6C, at pH 5.0-9.0 and in the presence of 0-2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C 15 : 0 (17.3 %), summed feature 3 (comprising iso-C 15 : 0 2-OH and/or C 16 : 1 v7c, 15.5 %) and C 16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100 T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA-DNA relatedness was 3.0 % with F. glaciei JCM 13953 T and 11.5 % with F. johnsoniae KACC 11410 T . On the basis of the evidence from this study, strain CS100 T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100 T (5KACC 15048 T 5JCM 17386 T ).

Flavobacterium plurextorum sp. nov. Isolated from Farmed Rainbow Trout (Oncorhynchus mykiss)

“…Flavobacterium species with 16S rRNA gene sequence similarities to the sequences of the trout strains lower than 98.0% correlated with levels of genomic DNA-DNA relatedness always lower than 70% [9]–[11], [34]–[36]. For that reason, DNA-DNA hybridizations were carried out only between strain 1126-1H-08 T and the type strains of the phylogenetically closest related species; i.e.…”

Flavobacterium tyrosinilyticum sp. nov., isolated from the rhizosphere of wild strawberry

“…T . All data were obtained in this study, except the DNA G+C content of the reference strains (taken from Kim et al, 2011a, b;Lim et al, 2011;Yang et al, 2011;Ngo et al, 2015). All strains are catalase-and oxidase-positive and produce flexirubin-type pigments.…”

Flavobacterium verecundum sp. nov., isolated from a freshwater river

“…Members of the genus are physiologically diverse: they can be psychrophilic, psychrotolerant or mesophilic, and can be halophilic, halotolerant or sensitive to salts. Strains of members of the genus Flavobacterium have been reported from diverse habitats such as animals, plants, freshwater, seawater, sediment, soil, glacier, Antarctic lakes and wastewater treatment plants (Zhang et al, 2010;Lim et al, 2011;Sheu et al, 2011Sheu et al, , 2013Dong et al, 2012Dong et al, , 2013K€ ampfer et al, 2012;Chen et al, 2013;Glaeser et al, 2013;Joung et al, 2013;Kang et al, 2013;Kuo et al, 2013;Fujii et al, 2014;Li et al, 2014).…”