Flavobacterium erciyesense sp. nov., a putative non-pathogenic fish symbiont

Satıcıoğlu, İzzet Burçin

doi:10.1007/s00203-021-02566-2

Cited by 12 publications

(4 citation statements)

References 48 publications

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“…Isolate CA 139 was closely related to F. bernardetii, a recently described species from diseased rainbow trout (O. mykiss) that has not been reported in the United States (Saticioglu et al, 2021b). Isolate OR 10-19 and CA 8 grouped with F. erciyesense and F. turcicum, previously associated with fish disease in Turkey (Saticioglu, 2021;Saticioglu et al, 2021a), while CA 44 grouped with F. aquaticum, which has only been reported in healthy catla (Catla catla) in India (Verma and Rathore, 2015). Isolates CA R1970 3 and CA 2 branched with F. bizetiae, F. araucananum and F. piscis, which have all been linked to fish disease (Table 1).…”

Section: Discussionmentioning

confidence: 78%

Atypical flavobacteria recovered from diseased fish in the Western United States

Heckman

Yazdi

Pomaranski

et al. 2023

Front. Cell. Infect. Microbiol.

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Flavobacterial diseases, caused by bacteria in the order Flavobacteriales, are responsible for devastating losses in farmed and wild fish populations worldwide. The genera Flavobacterium (Family Flavobacteriaceae) and Chryseobacterium (Weeksellaceae) encompass the most well-known agents of fish disease in the order, but the full extent of piscine-pathogenic species within these diverse groups is unresolved, and likely underappreciated. To identify emerging agents of flavobacterial disease in US aquaculture, 183 presumptive Flavobacterium and Chryseobacterium isolates were collected from clinically affected fish representing 19 host types, from across six western states. Isolates were characterized by 16S rRNA gene sequencing and phylogenetic analysis using the gyrB gene. Antimicrobial susceptibility profiles were compared between representatives from each major phylogenetic clade. Of the isolates, 52 were identified as Chryseobacterium species and 131 as Flavobacterium. The majority of Chryseobacterium isolates fell into six clades (A-F) consisting of ≥ 5 fish isolates with ≥ 70% bootstrap support, and Flavobacterium into nine (A-I). Phylogenetic clades showed distinct patterns in antimicrobial susceptibility. Two Chryseobacterium clades (F & G), and four Flavobacterium clades (B, G-I) had comparably high minimal inhibitory concentrations (MICs) for 11/18 antimicrobials tested. Multiple clades in both genera exhibited MICs surpassing the established F. psychrophilum breakpoints for oxytetracycline and florfenicol, indicating potential resistance to two of the three antimicrobials approved for use in finfish aquaculture. Further work to investigate the virulence and antigenic diversity of these genetic groups will improve our understanding of flavobacterial disease, with applications for treatment and vaccination strategies.

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Section: Discussionmentioning

confidence: 78%

Atypical flavobacteria recovered from diseased fish in the Western United States

Heckman

Yazdi

Pomaranski

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Researchers have suggested that certain types of bacteria, such as Flavobacterium , Erwinia , Formosa , and Muricauda , can produce zeaxanthin [ 23 , 34 , 46 ]. Flavobacterium strains obtained from various aquatic environments, such as the Antarctic and other glacial sites, have been recognized as potential candidates for producing zeaxanthin [ 47 - 50 ]. The study described the isolation of JSWR-1, which can produce zeaxanthin as the primary carotenoid.…”

Section: Discussionmentioning

confidence: 99%

Overproduction of Xanthophyll Pigment in Flavobacterium sp. JSWR-1 under Optimized Culture Conditions

Raman,

Ko,

Kim

et al. 2023

J. Microbiol. Biotechnol.

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Flavobacterium can synthesize xanthophyll, particularly the pigment zeaxanthin, which has significant economic value in nutrition and pharmaceuticals. Recently, the use of carotenoid biosynthesis by bacteria and yeast fermentation technology has shown to be very efficient and offers significant advantages in large-scale production, cost-effectiveness, and safety. In the present study, JSWR-1 strain capable of producing xanthophyll pigment was isolated from a freshwater reservoir in Wanju-gun, Republic of Korea. Based on the morphological, physiological, and molecular characteristics, JSWR-1 classified as belonging to the Flavobacterium species. The bacterium is strictly aerobic, Gram-negative, rod-shaped, and psychrophilic. The completed genome sequence of the strain Flavobacterium sp. JSWR-1 is predicted to be a single circular 3,425,829-bp chromosome with a G+C content of 35.2% and 2,941 protein-coding genes. The optimization of carotenoid production was achieved by small-scale cultivation, resulting in zeaxanthin being identified as the predominant carotenoid pigment. The enhancement of zeaxanthin biosynthesis by applying different light-irradiation, variations in pH and temperature, and adding carbon and nitrogen supplies to the growth medium. A significant increase in intracellular zeaxanthin concentrations was also recorded during fed-batch fermentation achieving a maximum of 16.69 ± 0.71 mg/l, corresponding to a product yield of 4.05 ± 0.15 mg zeaxanthin per gram cell dry weight. Batch and fed-batch culture extracts exhibit significant antioxidant activity. The results demonstrated that the JSWR-1 strain can potentially serve as a source for zeaxanthin biosynthesis.

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“…S-1 T was cultured routinely in TSA medium at 25 °C. All phenotypic tests were performed twice at different times on freshly prepared medium using standard methods as described previously [6,[34][35][36][37][38]. The Gram-staining was performed using a Gram-Stain kit (bioMérieux).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Shewanella oncorhynchi sp. nov., a novel member of the genus Shewanella, isolated from Rainbow Trout (Oncorhynchus mykiss)

Altun

Duman

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A strain, S-1T was isolated from rainbow trout (Oncorhynchus mykiss) exhibiting clinical symptoms of lens atrophy, inappetence, visual impairment and growth retardation. The strain was identified as representing a member of the genus Shewanella on the basis of the results of 16S rRNA gene sequence analysis. The neighbor-joining phylogenetic tree based on 16S rRNA gene sequences indicated that S-1T clustered with Shewanella putrefaciens JCM 20190T, Shewanella profunda DSM 15900T, and Shewanella hafniensis P010T, sharing 99.3, 98.8 and 87.7% 16S rRNA gene similarities, respectively. A polyphasic taxonomic approach including phenotypic, chemotaxonomic, and genomic characterization was employed to ascertain the taxonomic position of S-1T within the genus Shewanella . The overall genome relatedness indices (OGRI) for S-1T compared with the most closely related type strains S. hafniensis ATCC BAA-1207T, Shewanella baltica NCTC 10735T, S. putrefaciens ATCC 8071T and S. profunda DSM 15900T were calculated as 40.8, 40.1, 28.5 and 27.3% for digital DNA–DNA hybridization (dDDH), and 91.6, 91.0, 86.3 and 85.1% for average nucleotide identity (ANI), respectively. OGRI values between S-1T and its close neighbours confirmed that the strain represents a novel species in the genus Shewanella .The DNA G+C content of the strain is 45.2%. Major fatty acids were C17 : 1ω8c, C15 : 0iso, and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The predominant polar lipids were phosphatidylethanolamine, phospholipid, amino-phospholipid and unidentified lipids. The major respiratory quinones were ubiquinone-8, ubiquinone-7 and menaquinone-7. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name Shewanella oncorhynchi sp. nov. is proposed, with S-1T as the type strain (JCM 34183T= KCTC 82249T).

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Flavobacterium erciyesense sp. nov., a putative non-pathogenic fish symbiont

Cited by 12 publications

References 48 publications

Atypical flavobacteria recovered from diseased fish in the Western United States

Atypical flavobacteria recovered from diseased fish in the Western United States

Overproduction of Xanthophyll Pigment in Flavobacterium sp. JSWR-1 under Optimized Culture Conditions

Shewanella oncorhynchi sp. nov., a novel member of the genus Shewanella, isolated from Rainbow Trout (Oncorhynchus mykiss)

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