Flavone polyphenols dominate in Thymus schimperi Ronniger : LC–ESI–MS/MS characterization and study of anti-proliferative effects of plant extract on AGS and HepG2 cancer cells

Desta, Kebede Taye; Kim, Gon Sup; El‐Aty, A. M. Abd; Raha, Suchismita; Kim, Manbae; Jeong, Ji Hoon; Warda, Mohamad; Hacımüftüoğlu, Ahmet; Shin, Ho‐Chul; Shin, Sung Chul

doi:10.1016/j.jchromb.2017.03.035

Cited by 34 publications

(19 citation statements)

References 46 publications

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“…Also, a salvianolic acid F derivative and a salvianolic acid K isomer were detected but not quantified due to small amounts. The presence of salvianolic acid derivatives in other Thymus species has been previously described in the literature [9,12,[31][32][33][34][35]. Except for T. carnosus [9], and Thymus algeriensis [32], their portion is normally represented either as <5% of the total phenolic compounds extracted [12,31] or they could not be identified [13,17].…”

Section: Phenolic Compound Profiles In Aqueous Decoction and Hydroethanolic Extractsmentioning

confidence: 98%

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

et al. 2019

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Thymus mastichina, also called mastic thyme or Spanish marjoram, is endemic to the Iberian Peninsula, where it is widely used in folk medicine especially for treating digestive and respiratory systems disorders, and as a condiment to season olives. This work describes for the first time the detailed phenolic composition of exhaustive hydroethanolic extracts and aqueous decoctions of Thymus mastichina. Unlike other species of the Thymus genera, Thymus mastichina extracts contain high amounts of salvianolic acid derivatives, with salvianolic acid A isomer being the main derivative. This isomer was identified in extracts from Thymus mastichina for the first time. Also, an undescribed salvianolic acid derivative in Thymus mastichina was identified and its structure was tentatively described. Extracts from Thymus mastichina showed significant scavenging activity of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation, hydroxyl, and nitric oxide radicals. The anti-proliferative effect of both T. mastichina extracts were tested against Caco-2 and HepG2 cells; the hydroethanolic extract showed a high anti-proliferative activity against Caco-2 cells compared to HepG2 cells (at 24 h exposure, the concentration that inhibits 50% of proliferation, IC50, was 71.18 ± 1.05 µg/mL and 264.60 ± 11.78 µg/mL for Caco-2 and HepG2, respectively). Thus, these results make this species a promising candidate for further investigation of its anti-tumoral potential. Therefore, Thymus mastichina can be potentially used as a functional food (used as a decoction or herbal tea) or as a source of bioactive ingredients with antioxidant and anti-proliferative properties.

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Section: Phenolic Compound Profiles In Aqueous Decoction and Hydroethanolic Extractsmentioning

confidence: 98%

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

et al. 2019

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“…Notably, plant extracts (including T. vulgaris and T. serpyllum) proved to be more toxic against the cancer resistant cells than the analyzed phenolics (that exhibited greater toxicity against the non-resistant cells), suggesting the existence of synergistic or additive effects between the extracts' components [89] (Table 3). T. citriodorus Moreover, methanolic extracts of Thymus schimperi, containing high amounts of the flavone luteolin and derivatives, required doses of 50-100 µg/mL to inhibit the growth of human gastric adenocarcinoma AGS cells by 50% (IC 50 = 88 µg/mL) [91]. In addition, Galasso et al [42] reported that T. longicaulis extracts, containing high amounts of quercetin-O-hexoside, luteolin-O-hexoside and salvianolic acid K, caused a decrease of cell viability on breast cancer MDA-MB-23, colon carcinoma HCT-116 and lung fibroblast MRC-5 cells, equal to or even higher than those observed for vinblastine, a known anti-cancer agent [25].…”

Section: Anticancer Activitymentioning

confidence: 99%

“…Moreover, methanolic extracts of Thymus schimperi , containing high amounts of the flavone luteolin and derivatives, required doses of 50–100 μg/mL to inhibit the growth of human gastric adenocarcinoma AGS cells by 50% (IC 50 = 88 μg/mL) [ 91 ]. In addition, Galasso et al [ 42 ] reported that T. longicaulis extracts, containing high amounts of quercetin- O -hexoside, luteolin- O -hexoside and salvianolic acid K, caused a decrease of cell viability on breast cancer MDA-MB-23, colon carcinoma HCT-116 and lung fibroblast MRC-5 cells, equal to or even higher than those observed for vinblastine, a known anti-cancer agent [ 25 ].…”

Section: Anticancer Activitymentioning

confidence: 99%

Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

2020

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Thymus genus comprises numerous species that are particularly abundant in the West Mediterranean region. A growing body of evidence suggests that many of these species are a rich source of bioactive compounds, including phenolic compounds such as rosmarinic acid, salvianolic acids and luteolin glycosides, able to render them potential applications in a range of industrial fields. This review collects the most relevant studies focused on the antioxidant, anti-inflammatory and anti-cancer of phenolic-rich extracts from Thymus plants, highlighting correlations made by the authors with respect to the main phenolic players in such activities.

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“…Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) is one of the top choices for analysis of plant polyphenols in complex mixtures due to its high sensitivity and efficiency (Desta et al, 2017). Analyses of bioactive compounds isolated from the endophytic fungi of the artichoke were carried out with Triple Quadrupole LC-DAD-ESI-MS/MS, Agilent 6420 Series, system which consisted of a capillary HPLC equipped with a binary pump solvent delivery, thermostatic column compartment, diode array detector (DAD), and a QQQ 6420 mass detector coupled with a pneumatic nebulizer-assisted electrospray interface (ESI).…”

Section: Liquid Chromatography-electrospray Ionization Tandem Mass Spmentioning

confidence: 99%

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

2018

Global NEST: The International Journal

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Plant-associated microorganisms, especially endophytic fungi, represent an untapped resource for the discovery of biologically active natural products. The objectives of this study were to isolate, identify endophytic fungi, and produce their bioactive metabolites from the leaves of two varieties of Egyptian artichoke namely: French Hyrious and Egyptian Baladi. In addition, assess of their total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC). The results of this novel study show a total of 35 endophytic fungal species belonging to 14 genera were isolated from both artichoke leaves with gross total counts of colonizing endophytic fungi ranged from 71 to 123 cfu which is matching 78.89% to 136.67% of colonization frequency. All taxa recovered were assigned to Ascomycetes. In addition, there is high species richness and diversity indices of endophytic filamentous fungi in the leaves Baladi Artichoke as compared to its French rival.  Alternaria alternata were found to be the most frequently isolated dominant species. The TAC, TPC and TFC of the fungal cultures ranged from 163 to 681 mgAAE/gDW, 10.38 to 40.30 mgGAE/ gDW, and 13.92 to 173.55 mgQE/gDW, respectively. Furthermore, LC-ESI-MS/MS conﬁrmed the presence of 1,3-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid in the methanolic extract of A. alternata. Hence, this novel study suggested that the metabolites produced by endophytic fungi associated with Egyptian artichoke could be explored as an economic and potential natural resources with diverse pharmaceutical and biological activities.

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Flavone polyphenols dominate in Thymus schimperi Ronniger : LC–ESI–MS/MS characterization and study of anti-proliferative effects of plant extract on AGS and HepG2 cancer cells

Cited by 34 publications

References 46 publications

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials

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