Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Abstract

It is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography-diode array detection analysis method for the simultaneous detection of myricetin-3-O-b-Dgalactopyranoside, myricetin-3-O-a-L-arabinopyranoside, and myricetin-3-O-a-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83-107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.

Cyperus esculentusL. (tigernut) mitigates high salt diet‐associated testicular toxicity in Wistar rats by targeting testicular steroidogenesis, oxidative stress and inflammation

“…Dried tubers of Cyperus esculentus (tigernuts) were purchased from Bogobiri market, Calabar, Nigeria and authenticated in the Herbarium Unit of the Department of Botany, University of Calabar. Extraction was done following previously described method (Costa et al,2014) with slight modification. After removing bad tubers and dirt, the good ones were washed clean, air‐dried and crushed into fine powder using a blender (Kenwood 1.6 L, BL480 Prestons, Australia) for 10 min.…”

Pouteria ramiflora (Mart.) Radlk. extract: Flavonoids quantification and chemopreventive effect on HepG2 cells

“…activity. Although several extracts of different Pouteria species were previously chemically characterized, only the extract of P. torta was evaluated for its possible mutagenic activity, where Costa et al (2014) demonstrated mutagenicity for Salmonella thyphimurium and in rats. Thus far, there are no apparent data available regarding the assessment of P. ramiflora extract for mutagenic and antimutagenic actions in mammalian cells in vitro.…”

Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection

“…Traditionally, the flavonoids could be separated by the means of HPLC , GC and so on. Compared with all these means, CE analysis is much faster with less sample consumption that makes it very suitable for the analysis of these compounds .…”