Flavonoid Production, Growth and Differentiation of Stelechocarpus burahol (Bl.) Hook. F. and Th. Cell Suspension Culture

Habibah, Noor Aini; Moeljopawiro, Sukarti; Dewi, Kumala; Indrianto, Ari

doi:10.3923/pjbs.2017.197.203

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…Kinetin significantly increased the growth and differentiation of callus D. alata propagated in vitro with an optimal concentration of 2.5 mg/L [19]. Furthermore, 2,4-D acts like auxin, significantly affecting plant calluses' growth, such as Stelechocarpus burahol [20] and Elaeocarpus grandiflorus [21]. However, the effect of the 2,4-D mechanism on D. esculenta callus growth remains unclear.…”

Section: Resultsmentioning

confidence: 99%

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

Habibah,

Yuniastuti,

Nugrahaning

et al. 2024

Trends Sci

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The callus culture of Dioscorea esculenta is a potential strategy to produce efficacious bioactive compounds in multiplied production. It can be carried out by applying plant growth regulators (PGR) and environmental modification, including light-emitting diodes (LEDs) exposure. This study aims to investigate the impact of PGR and the stimulatory effect of LEDs on flavonoid profiles in D. esculenta callus culture. The study was conducted using combination variations of 2,4-D (0.5 and 1 ppm), kinetin (0.5 and 1 ppm), and LEDs stimulatory conditions (light and dark). The results showed that flavonoids are the dominant compounds found in lesser yam cultures, which reached more than 78 % in all treatments-specifically, adding 0.5 ppm kinetin and 0.5 ppm 2,4-D to D. esculenta callus cultures significantly increased flavonoids production compared to single PGR treatment. This shows there is a synergistic activity induced by 2,4-D and kinetin. Furthermore, monochromatic light exposure also significantly affects flavonoid production. Analysis of 5 main flavonoids, isoflavones, flavanones, kaempferols, quercetin, and epigallocatechin, proved that monochromatic light significantly increased flavonoids production compared to callus cultures were kept in a growth room under 24 °C temperature in the dark condition. This study confirmed that equally combining 0.5 ppm of 2,4-D and 0.5 ppm of kinetin under monochromatic LEDs increases flavonoid production in D. esculenta callus cultures. Further research must be conducted to describe how the bioactive compound synthesis signaling pathway involving PGR and monochromatic LEDs improves plant quality in the pharmaceuticals’ role. HIGHLIGHTS Kinetin and 2,4-D at the same concentration of 0.5 ppm is an optimal combination to increase secondary metabolite production in esculenta callus culture Combining kinetin and 2,4-D at low concentrations significantly increased flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin in esculenta callus culture Light condition significantly affects the production of flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin There is evidence that kinetin and 2,4-D under light conditions work in a synergic effect on the biosynthesis of secondary metabolite compounds in esculenta callus cultures GRAPHICAL ABSTRACT

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Section: Resultsmentioning

confidence: 99%

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

Habibah,

Yuniastuti,

Nugrahaning

et al. 2024

Trends Sci

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“…Previous studies have reported that 8 th weeks of Taraxacum officinale during exponential growth produced higher triterpene yield than stationary phase (Martínez et al, 2023). Furthermore, flavonoid production from Stelechocarpus burahol cell suspension culture showed higher yield during the cell growth in the log phase (Habibah et al, 2017). Based on our best knowledge, there is no information regarding the effect of callus age (growth phase) on flavonoid production in Talinum paniculatum in vitro culture.…”

Section: Introductionmentioning

confidence: 88%

“…This is might be in the exponential phase, the callus is actively dividing and increasing biomass so that in this phase the flavonoids synthesized are also higher (Tan et al, 2010). Furthermore, Habibah et al (2017) also reported that flavonoid production from Stelechocarpus burahol cell suspension culture showed higher yield during the cell growth in the log phase than stationary phase. This research also reported that the production of flavonoids in the cell suspension cultures of S. burahol was not in line with its growth.…”

Section: Jurnal Pembelajaran Dan Biologimentioning

confidence: 96%

The Effect of Callus Age on Biomass and Flavonoid Production in Talinum paniculatum In Vitro Culture

Restiani,

Aditiyarini,

Dolonseda

et al. 2024

JPBN

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Talinum paniculatum (Javanese Ginseng) is an herbaceous plant belonging to Talinaceae family that has been known as an important medicinal plant. Flavonoid is one of the prominent bioactive compounds that are contained in all parts of T. paniculatum and has been known for its several bioactivities, including antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and anti-cancer. Callus culture can be used to ensure the quantity and quality of flavonoid production. Callus age is one of the important factors that needs to be considered in order to produce optimum biomass and flavonoid production. Therefore, this research aimed to investigate the effect of callus age on biomass and flavonoid production in T. panicultaum in vitro culture. Young leaves were used as an explant, and callus induction was performed using MS solid media supplemented with kinetin 3 mg/L and 2.4-D 2 mg/L. Biomass and flavonoid production were observed during 0, 28, 35, 42, and 49 days. Results showed that biomass and flavonoid production were influenced by callus age. The production of flavonoid in T. paniculatum callus culture was not linear with the growth of callus (biomass), and the optimum flavonoid production resulted in the 28th days of callus age (49.100 mg QE/g), while the optimum biomass resulted in the 49th days of callus age (4.295 g). It can be concluded that the optimum conditions for biomass and flavonoid production in T. paniculatum callus culture are influenced by different callus ages

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“…Ex situ conservation attempts using in vitro culture can be used to effectively propagate this plant. Several studies related to in vitro culture of Kepel plants have been conducted with several focuses, including optimization of sterilization methods for leaf explants (Habibah et al, 2013), nodes (Hutabarat et al, 2022) and endosperm (Handayani et al, 2022), optimization of PGR that supports callus growth of Kepel seed and fruit mesocarp explants (Habibah et al, 2016(Habibah et al, , 2017(Habibah et al, , 2019, and nodal explant (Sekar et al, 2023), and optimization of flavonoid production from callus and cell suspension cultures of Kepel plants (Habibah et al, 2016(Habibah et al, , 2019. However, in vitro propagation of Kepel plants still faces obstacles, including the browning of explants.…”

Section: Introductionmentioning

confidence: 99%

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

Sibarani,

Restiani,

Prasetyaningsih

2024

JPBN

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Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol

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Flavonoid Production, Growth and Differentiation of Stelechocarpus burahol (Bl.) Hook. F. and Th. Cell Suspension Culture

Abstract: The results show that flavonoid production, growth and cell differentiation of a S. burahol cell suspension culture differed according to the culture age.

Cited by 8 publications

References 18 publications

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

The Effect of Callus Age on Biomass and Flavonoid Production in Talinum paniculatum In Vitro Culture

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

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