Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS

Pilepić, Kroata Hazler; Yang, Zongling; Jiang, Chen; Chen, Xianqiang; Wang, Ying; Zhao, Jing; Mihaljević, Snježana; Li, Shao‐Ping

doi:10.1016/j.ijms.2018.09.026

Cited by 10 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 18 alkaloids were identified in this study, corresponding to compounds 36,45,46,54,57,59,71,79,84,88,95,97,98,106,109,112,114, and 116 in Figure 3, and the mass spectrum information in . It is presumed to be cadambine, and its mass fragmentation pattern is shown in Figure 10B [26,36,41,42].…”

Section: Characterization Of Alkaloidsmentioning

confidence: 97%

“…Then continues to remove one molecule of CO (28 Da) to obtain m/z 227.0732 fragment ion, and m/z 301.0441 to remove C 8 H 6 O 3 (150 Da) to obtain m/z 151.0286 fragment ions. It is presumed to be rutin, and its mass fragmentation pattern is shown inFigure 12B[28,34,38,[46][47][48][49]. According to the fragment ions of these compounds, the fragment ions (m/z 133[C 8 H 5 O 2 ] -, m/z 135 [C 8 H 7 O 2 ] -, m/z 151 [C 7 H 3 O 4 ] -, m/z 153 [C 7 H 5 O 4 ] + )are characteristic fragment ions of flavonoids.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Wang

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

Duzhong Jiangya tablet is a hypotensive drug. In this study, high-performance liquid chromatography-Fourier transform-ion cyclotron resonance-mass spectrometry technology was used to quickly identify its chemical composition. SinoChrom ODS-BP column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile(A)-0.1% formic acid solution(B). The flow rate was 1 mL/min. Extracted ion chromatogram was used to analyze the samples in positive and negative ion modes. Based on the accurate mass spectrometry information (such as quasi-molecular ions and fragment ions) obtained from the instrument, combined with reference compounds and literature, the chemical composition of Duzhong Jiangya Tablets was identified. A total of 131 compounds were identified, including four types of penylpropanoids, six types of phenylethanoid glycosides, 10 types of organic acids, 14 types of iridoids, 12 types of lignans, 18 types of alkaloids, seven types of coumarins, and 60 kinds of flavonoids. This established method can quickly and efficiently identify chemical constituents in Duzhong Jiangya tablet, lay a foundation for the research on the efficacy and quality of this traditional Chinese medicine, and provide a reference for the characterization of the chemical constituents of other traditional Chinese medicine preparations. K E Y W O R D S chemical composition, Duzhong Jiangya Tablet, Fourier transform ion cyclotron resonance, traditional Chinese medicine 1 INTRODUCTION Varieties of medicinal materials in traditional Chinese medicine compound preparations have complex chemical components. The synergy between the chemical components is the material basis for the traditional Chinese medicine preparations to exert their pharmaco-Article Related Abbreviations: ICR, ion cyclotron resonance logical effects [1]. The active ingredients of most Chinese medicines are still unknown, although there are many reports on Chinese medicine research. Therefore, it is very important to establish a fast and efficient method to identify the chemical components in traditional Chinese medicine. This can reasonably explain the material basis of the efficacy of traditional Chinese medicine. Duzhong Jiangya Tablet consists of Eucommia ulmoides Oliv, Leonurus japonicus Houtt, Prunella vulgaris L,

show abstract

Section: Characterization Of Alkaloidsmentioning

confidence: 97%

mentioning

confidence: 99%

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Wang

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Fifty-one prenyl flavonoid glycosides, 18 phenolic acids, and 42 icariin analogues were successfully identified or tentatively identified from Epimedium koreanum using this method ( Wang et al, 2010 ; Yang et al, 2017b ). Meanwhile, more than 100 compounds were also identified or tentatively identified from Epimedii Folium based on the MS/MS of standards, and the compounds were compared with reference results by the UHPLC-HRMS method ( Wang Y. et al, 2017 ; Pilepić et al, 2018 ; Li M. Y. et al, 2020 ; Li N. et al, 2020 ). Polygonum multiflorum Thunb.…”

Section: Application Of Uhplc-hrmsmentioning

confidence: 99%

The Application of UHPLC-HRMS for Quality Control of Traditional Chinese Medicine

Shi

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

UHPLC-HRMS (ultra-high-performance liquid chromatography-high resolution mass spectrometry) is a new technique that unifies the application of UHPLC with HRMS. Because of the high sensitivity and good separation ability of UHPLC and the sensitivity of HRMS, this technique has been widely used for structure identification, quantitative determination, fingerprint analysis, and elucidation of the mechanisms of action of traditional Chinese medicines (TCMs) in recent years. This review mainly outlines the advantages of using UHPLC-HRMS and provides a survey of the research advances on UHPLC-HRMS for the quality control of TCMs.

show abstract

“…In addition, they are used in the treatment of osteoporosis, rheuma, hepatitis, leukopenia, car-diovascular problems, infertility in Europe, Korea, Japan and China. [4,5] Many dietary supplement prepared from Epimedium plants are commercially available for aphrodisiac, tonic and antirheumatic effects. [6] More than 260 chemically active components have been identified in Epimedium species.…”

Section: Introductionmentioning

confidence: 99%

“…Aerial parts and roots of plants belonging to the Epimedium genus are traditionally employed in the treatment of respiratory system disease such as asthma and lung cancer, as well as being used as a diaphoretic. In addition, they are used in the treatment of osteoporosis, rheuma, hepatitis, leukopenia, cardiovascular problems, infertility in Europe, Korea, Japan and China [4,5] . Many dietary supplement prepared from Epimedium plants are commercially available for aphrodisiac, tonic and antirheumatic effects [6] .…”

Section: Introductionmentioning

confidence: 99%

Insights into the Biological Activity and Cytotoxic Mechanism of Epimedium pubigerum

Seyhan,

Akkaya,

Kolci

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

In this work, the phytochemical characterization, biological activity, and cytotoxic mechanism of aerial and rhizome methanol extracts (SME and RME) of Epimedium pubigerum were investigated to demonstrate its potential usage in the treatment of lung cancer. LC‐HRMS analysis, total phenolic/flavonoid content assay, DPPH radical scavenging assay, DNA interaction, cytotoxicity, and western blotting were investigated using different methods. Fumaric acid was found to be the most abundant compound in both extracts. SME and RME were cytotoxic on A549 cells concentration‐dependently. Also, in vitro scratch assay showed that SME and RME led to a significant anti‐migratory effect at 1 mg/mL. Cytochrome c, p53, and caspase 3 expression significantly increased in the presence of RME compared to the control. All of these results claimed that RME might be suggested as a theoretically more effective phytotherapeutic agent for lung cancer compared to the effect seen with the SME.

show abstract

Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS

Cited by 10 publications

References 20 publications

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

The Application of UHPLC-HRMS for Quality Control of Traditional Chinese Medicine

Insights into the Biological Activity and Cytotoxic Mechanism of Epimedium pubigerum

Contact Info

Product

Resources

About